Genetic test for liver copper accumulation in dogs

Patent metadata

Field	Value
Publication number	US-12227805-B2
Application number	US-202117360692-A
Country	US
Kind code	B2
Filing date	Jun 28, 2021
Priority date	Dec 6, 2011
Publication date	Feb 18, 2025
Grant date	Feb 18, 2025

Abstract

The present disclosure provides methods of determining the susceptibility of a dog to liver copper accumulation, comprising detecting in a biological sample obtained from the dog the presence or absence in the genome of the dog of one or more polymorphisms, and methods of treating or breeding the dog based on such determination.

First claim

What is claimed is:

1. A method of treating or breeding a dog, comprising:
(a) genotyping a biological sample obtained from the dog to determine in a genome of the dog presence or absence of a polymorphism of Chr22_3167534 (position 101 of SEQ ID NO: 144; and
(b) administering to the dog a low copper food stuff or a therapeutic amount of a copper chelator when the polymorphism is present; or
breeding the dog when the polymorphism is absent.

2. The method of claim 1 , further comprising genotyping the biological sample obtained from the dog to determine presence or absence of one or more polymorphisms selected from the group consisting of BICF2P506595 (position 61 of SEQ ID NO:1), BICF2P772765 (position 61 of SEQ ID NO:2), BICF2S2333187 (position 61 of SEQ ID NO:3), BICF2P1324008 (position 61 of SEQ ID NO: 4), BICF2P591872 (position 61 of SEQ ID NO:5), ATP7a_Reg4_F_9 (position 164 of SEQ ID NO: 131), UBL5_Reg1F_16 (position 97 of SEQ ID NO: 132), golga5_Reg1_24 (position 70 of SEQ ID NO: 133), golga5_26 (position 88 of SEQ ID NO: 134), golga5_27 (position 104 of SEQ ID NO: 135), golga5_28 (position 139 of SEQ ID NO: 136), golga5_29 (position 128 of SEQ ID NO: 137), golga5_30 (position 95 of SEQ ID NO: 138), golga5_31 (position 106 of SEQ ID NO: 139), atp7areg17_32 (position 95 of SEQ ID NO: 140), and atp7areg17_33 (position 90 of SEQ ID NO: 141).

3. The method of claim 1 , further comprising genotyping the biological sample obtained from the dog to determine in the genome of the dog the presence or absence of one or more polymorphisms selected from the group consisting of Chr10_65209946 (position 101 of SEQ ID NO: 155), Chr22_3135144 (position 101 of SEQ ID NO: 145), Chr20_55461150 (position 101 of SEQ ID NO: 146), Chr19_6078084 (position 101 of SEQ ID NO: 148), Chr3_86838677 (position 101 of SEQ ID NO: 152), and ChrX_63338063 (position 102 of SEQ ID NO: 142).

4. The method of claim 1 , wherein the dog has genetic inheritance of the Labrador Retriever breed.

5. The method of claim 1 , further comprising genotyping the biological sample obtained from the dog to determine in the genome of the dog the presence or absence of one or more polymorphisms selected from the group consisting of Chr22_3135144 (position 101 of SEQ ID NO: 145), Chr20_55461150 (position 101 of SEQ ID NO: 146), ChrX 120879711 (position 101 of SEQ ID NO: 147), Chr19_6078084 (position 101 of SEQ ID NO: 148), Chr15_62625262 (position 101 of SEQ ID NO: 149), Chr14_39437543 (position 101 of SEQ ID NO: 150), Chr15 62625024 (position 101 of SEQ ID NO: 151), Chr3 86838677 (position 101 of SEQ ID NO: 152), Chr24_4011833 (position 101 of SEQ ID NO: 153), Chr18_60812198 (position 101 of SEQ ID NO: 154), CGCCCC repeat at chromosome location 22 starting at genomic location 3135287, Chr32_38904515 (position 101 of SEQ ID NO: 156), Chr8_4892743 (position 101 of SEQ ID NO: 157), Chr8_4880518 (position 101 of SEQ ID NO: 158), ChrX 63338063 (position 102 of SEQ ID NO: 142), BICF2P506595 (position 61 of SEQ ID NO: 1), BICF2P772765 (position 61 of SEQ ID NO:2), BICF2S2333187 (position 61 of SEQ ID NO: 3), BICF2P1324008 (position 61 of SEQ ID NO:4), BICF2P591872 (position 61 of SEQ ID NO: 5), ATP7a_Reg4_F_9 (position 164 of SEQ ID NO: 131), UBL5_Reg1F_16 (position 97 of SEQ ID NO: 132), golga5_Reg1_24 (position 70 of SEQ ID NO: 133), golga5_26 (position 88 of SEQ ID NO: 134), golga5_27 (position 104 of SEQ ID NO: 135), golga5_28 (position 139 of SEQ ID NO: 136), golga5_29 (position 128 of SEQ ID NO: 137), golga5_30 (position 95 of SEQ ID NO: 138), golga5_31 (position 106 of SEQ ID NO: 139), atp7areg17_32 (position 95 of SEQ ID NO: 140), and atp7areg17_33 (position 90 of SEQ ID NO: 141).

6. A method of treating or breeding a dog, comprising:
(a) inputting to a computer system genotyping data concerning presence or absence in a genome of the dog a polymorphism of Chr22_3167534 (position 101 of SEQ ID NO: 144);
(b) comparing the genotyping data to a computer database, wherein the computer database comprises information of the risk of dog liver copper accumulation associated with a polymorphism of Chr22_3167534 (position 101 of SEQ ID NO: 144);
(c) determining whether the dog is at risk of liver copper accumulation based on the comparison; and
(d) administering to the dog a low copper food stuff or a therapeutic amount of a copper chelator if the dog is determined to be at risk of liver copper accumulation, or
breeding the dog if the dog is determined to be not at risk of liver copper accumulation.

Assignees

• Mars Inc

Inventors

• Martin Alan James
• Jones Paul Glyn
• Watson Adrian
• Rothuizen Jan
• Fieten Hille
• Leegwater Pieter Antonius Jozef

Classifications

• C12Q2600/124

  Animal traits, i.e. production traits, including athletic performance or the like · CPC title

• A01K67/02 Primary

  Breeding vertebrates · CPC title

• G16B20/10

  Ploidy or copy number detection · CPC title

• G16B20/40

  Population genetics; Linkage disequilibrium · CPC title

• G16B20/20

  Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection · CPC title