Method for enhancing expression of insulin like growth factor 1 receptor in umbilical cord mesenchymal stem cell expressing IGF1R and method for obtaining multipotent umbilical cord mesenchymal stem cell

Patent metadata

Field	Value
Publication number	US-12226437-B2
Application number	US-202217981736-A
Country	US
Kind code	B2
Filing date	Nov 7, 2022
Priority date	Jul 22, 2015
Publication date	Feb 18, 2025
Grant date	Feb 18, 2025

Abstract

A method for enhancing an expression of insulin like growth factor 1 receptor in an umbilical cord mesenchymal stem cell is provided. The method includes culturing the umbilical cord mesenchymal stem cell expressing insulin-like growth factor 1 receptor in a medium containing platelet-derived growth factor BB (PDGF-BB) to enhance the expression of insulin like growth factor 1 receptor in the umbilical cord mesenchymal stem cell.

First claim

What is claimed is:

1. A method for enhancing an expression of insulin like growth factor 1 receptor (IGF1R) in an umbilical cord mesenchymal stem cell expressing IGF1R, the method comprising:
deriving a plurality of umbilical cord mesenchymal stem cells from a Wharton's jelly;
isolating the umbilical cord mesenchymal stem cell expressing IGF1R from the plurality of umbilical cord mesenchymal stem cells by using an IGF1R antibody; and
culturing the umbilical cord mesenchymal stem cell expressing IGF1R in a medium containing platelet-derived growth factor BB (PDGF-BB) at 2.5-50 ng/ml to enhance the expression of IGF1R in the umbilical cord mesenchymal stem cell expressing IGF1R.

2. The method of claim 1 , wherein a source of the PDGF-BB is human cord blood serum.

3. The method of claim 1 , wherein a concentration of the PDGF-BB in the medium is 5-50 ng/ml.

4. The method of claim 1 , wherein the medium further comprises a cytokine selected from a group consisting of epidermal growth factor (EGF), angiogenin (ANG), macrophage inflammatory protein-5 (MIP-1δ), regulated on activation, normal T-cell expressed and presumably secreted (RANTES), and a combination thereof.

Assignees

• Univ China Medical

Inventors

• Shyu Woei-Cherng
• Lin Chen-Huan
• Lee Wei
• Hsieh Chia-Hung
• Hsu Chung-Y
• TSAI Chang-Hai

Classifications

• C12N5/0605

  Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly · CPC title

• C12N5/0668

  Mesenchymal stem cells from other natural sources · CPC title

• A61K38/1858

  Platelet-derived growth factor [PDGF] · CPC title

• A61P25/28

  for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia · CPC title

• C12N2500/84

  from mammals · CPC title