Cellulolytic enzyme compositions and uses thereof

Patent metadata

Field	Value
Publication number	US-12195776-B2
Application number	US-202117547836-A
Country	US
Kind code	B2
Filing date	Dec 10, 2021
Priority date	Aug 24, 2011
Publication date	Jan 14, 2025
Grant date	Jan 14, 2025

Abstract

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

First claim

What is claimed is:

1. An enzyme composition, comprising:
(i) a cellobiohydrolase I (CBHI) having at least 80% sequence identity to the amino acid sequence of the mature polypeptide of SEQ ID NO: 2;
(ii) a beta-glucosidase having at least 80%, but less than 100%, sequence identity to the amino acid sequence of the mature polypeptide of SEQ ID NO: 6 and comprising a substitution at one or more positions corresponding to positions 100, 283, 456, and 512 of the mature polypeptide of SEQ ID NO: 6, wherein the substitution at position 100 is Asp, the substitution at position 283 is Gly, the substitution at position 456 is Glu, and the substitution at position 512 is Tyr; and
(iii) an endoglucanase.

2. The enzyme composition of claim 1 , wherein the CBHI has at least 85% sequence identity to the amino acid sequence of the mature polypeptide of SEQ ID NO: 2.

3. The enzyme composition of claim 1 , wherein the CBHI has at least 90% sequence identity to the amino acid sequence of the mature polypeptide of SEQ ID NO: 2.

4. The enzyme composition of claim 1 , wherein the CBHI has at least 95% sequence identity to the amino acid sequence of the mature polypeptide of SEQ ID NO: 2.

5. The enzyme composition of claim 1 , wherein the CBHI comprises the amino acid sequence of the mature polypeptide of SEQ ID NO: 2.

6. The enzyme composition of claim 1 , wherein the beta-glucosidase has at least 85%, but less than 100%, sequence identity to the amino acid sequence of the mature polypeptide of SEQ ID NO: 6 and comprises a substitution at one or more positions corresponding to positions 100, 283, 456, and 512 of the mature polypeptide of SEQ ID NO: 6, wherein the substitution at position 100 is Asp, the substitution at position 283 is Gly, the substitution at position 456 is Glu, and the substitution at position 512 is Tyr.

7. The enzyme composition of claim 1 , wherein the beta-glucosidase has at least 90%, but less than 100%, sequence identity to the amino acid sequence of the mature polypeptide of SEQ ID NO: 6 and comprises a substitution at one or more positions corresponding to positions 100, 283, 456, and 512 of the mature polypeptide of SEQ ID NO: 6, wherein the substitution at position 100 is Asp, the substitution at position 283 is Gly, the substitution at position 456 is Glu, and the substitution at position 512 is Tyr.

8. The enzyme composition of claim 1 , wherein the beta-glucosidase has at least 95%, but less than 100%, sequence identity to the amino acid sequence of the mature polypeptide of SEQ ID NO: 6 and comprises a substitution at one or more positions corresponding to positions 100, 283, 456, and 512 of the mature polypeptide of SEQ ID NO: 6, wherein the substitution at position 100 is Asp, the substitution at position 283 is Gly, the substitution at position 456 is Glu, and the substitution at position 512 is Tyr.

9. The enzyme composition of claim 1 , wherein the beta-glucosidase comprises a substitution at one or more positions of positions 100, 283, 456, and 512 of the mature polypeptide of SEQ ID NO: 6, wherein the substitution at position 100 is Asp, the substitution at position 283 is Gly, the substitution at position 456 is Glu, and the substitution at position 512 is Tyr.

10. The enzyme composition of claim 1 , wherein the endoglucanase is a Trichoderma reesei endoglucanase.

11. The enzyme composition of claim 10 , wherein the endoglucanase is Trichoderma reesei endoglucanase I.

12. The enzyme composition of claim 10 , wherein the endoglucanase is Trichoderma reesei endoglucanase II.

13. The enzyme composition of claim 1 , which further comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

14. A process for degrading a cellulosic material, comprising: contacting the cellulosic material with the enzyme composition of claim 1 .

15. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with the enzyme composition of claim 1 ;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.

16. A process of fermenting a cellulosic material, comprising: fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with the enzyme composition of claim 1 .

Assignees

• Novozymes Inc

Inventors

• Shasky Jeffrey
• Fischer Amanda
• Maiyuran Suchindra

Classifications

• C12Y302/01176

  Cellulose 1,4-beta-cellobiosidase (reducing end)(3.2.1.176) · CPC title

• C12Y302/01091

  Cellulose 1,4-beta-cellobiosidase (3.2.1.91) · CPC title

• C12Y302/01037

  Xylan 1,4-beta-xylosidase (3.2.1.37) · CPC title

• C12Y302/01008

  Endo-1,4-beta-xylanase (3.2.1.8) · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title