Aqueous two-phase system nanofilter and separation method using same

Patent metadata

Field	Value
Publication number	US-12194415-B2
Application number	US-202117316864-A
Country	US
Kind code	B2
Filing date	May 11, 2021
Priority date	Nov 19, 2018
Publication date	Jan 14, 2025
Grant date	Jan 14, 2025

Abstract

Proposed are an aqueous two-phase system nanofilter capable of effectively separating particles having different sizes within a short time, and a separation method using same. The aqueous two-phase system nanofilter can separate mixed nanoparticles having a size difference of approximately 10 nm, through the designing of a composition constituting each composition. The separated nanoparticles can be applied across industries such as electronics, photoelectronics and magnetic fields, biomedical, medical and cosmetic fields, energy, catalysts, and structures.

First claim

The invention claimed is:

1. An aqueous two-phase system nanofilter, comprising:
a first aqueous phase forming a dispersion phase, and a second aqueous phase phase-separated from the dispersion phase and forming a continuous phase, so as to separate mixed nanoparticles having different sizes,
wherein the first aqueous phase flows in the continuous phase in a bulk form, and
wherein tension (γ) of an interface at which the first aqueous phase and the second aqueous phase are phase-separated satisfies the following Equation 1:
2×10 −7 J/m 2 ≤γ≤50×10 −5 J/m 2 .  [Equation 1]

2. The aqueous two-phase system nanofilter of claim 1 , wherein the aqueous two-phase system nanofilter separates the nanoparticles by moving the nanoparticles from the first aqueous phase to the second aqueous phase through diffusion.

3. The aqueous two-phase system nanofilter of claim 1 , wherein a pore size of a filter formed on the interface ranges from 1 to 500 nm.

4. The aqueous two-phase system nanofilter of claim 1 , wherein a pore size of a filter formed on the interface ranges from 30 to 80 nm.

5. The aqueous two-phase system nanofilter of claim 1 , wherein the first aqueous phase and the second aqueous phase are any one selected from a polymer, a polymer salt, or an aqueous solution in which a salt is dissolved.

6. The aqueous two-phase system nanofilter of claim 5 , wherein the polymer is one or more types selected from a group consisting of:
one type of hydrophilic polymer selected from a group consisting of polyarginine, polylysine, polyethylene glycol, polypropylene glycol, polyethyleneimine, chitosan, protamin, polyvinyl acetate, hyaluronic acid, chondroitin sulfate, heparin, alginate, hydroxyoxypropyl methylcellulose, gelatin, starch, poly(vinyl methyl ether), polyvinylpyrrolidone, and a combination thereof;
one type of polymer polysaccharide selected from a group consisting of cyclodextrin, glucose, dextran, mannose, sucrose, trehalose, maltose, ficoll, inositol, mannitol, sorbitol, sucrose-mannitol, glucose-mannitol, trehalose-polyethylene glycol, sucrose-polyethylene glycol, sucrose-dextran, and a combination thereof; and
a combination thereof.

7. The aqueous two-phase system nanofilter of claim 5 , wherein the salt is one type selected from a group consisting of (NH 4 ) 2 SO 4 , Na 2 SO 4 , MgSO 4 , K 2 HPO 4 , KH 2 PO 4 , NaCl, KCl, NaBr, NaI, LiCl, n-Bu 4 NBr, n-Pr 4 NBr, Et 4 NBr, Mg(OH) 2 , Ca(OH) 2 , Na 2 CO 3 , ZnCO 3 , Ca 3 (PO 4 ) 2 , ZnCl 2 , (C 2 H 3 ) 2 Zn, CdCl 2 , HgCl 2 , CoCl 2 , (CaNO 3 ) 2 , BaCl 2 , MgCl 2 , PbCl 2 , AlCl 3 , FeCl 2 , FeCl 3 , NiCl 2 , AgCl, AuCl 3 , CuCl 2 , sodium dodecyl sulfate, sodium tetradecyl sulfate, dodecyltrimethylammonium bromide, dodecyltrimethylammonium chloride, tetradecyltrimethylammonium bromide, and a combination thereof.

8. The aqueous two-phase system nanofilter of claim 1 , wherein the first aqueous phase-the second aqueous phase are a combination of polymer-polymer, polymer-polymer salt, or polymer-high concentration salt.

9. The aqueous two-phase system nanofilter of claim 8 , wherein the polymer is one or more types selected from a group consisting of:
one type of hydrophilic polymer selected from a group consisting of polyarginine, polylysine, polyethylene glycol, polypropylene glycol, polyethyleneimine, chitosan, protamin, polyvinyl acetate, hyaluronic acid, chondroitin sulfate, heparin, alginate, hydroxyoxypropyl methylcellulose, gelatin, starch, poly(vinyl methyl ether), polyvinylpyrrolidone, and a combination thereof;
one type of polymer polysaccharide selected from a group consisting of cyclodextrin, glucose, dextran, mannose, sucrose, trehalose, maltose, ficoll, inositol, mannitol, sorbitol, sucrose-mannitol, glucose-mannitol, trehalose-polyethylene glycol, sucrose-polyethylene glycol, sucrose-dextran, and a combination thereof; and
a combination thereof.

10. The aqueous two-phase system nanofilter of claim 8 , wherein the salt is one type selected from a group consisting of (NH 4 ) 2 SO 4 , Na 2 SO 4 , MgSO 4 , K 2 HPO 4 , KH 2 PO 4 , NaCl, KCl, NaBr, NaI, LiCl, n-Bu 4 NBr, n-Pr 4 NBr, Et 4 NBr, Mg(OH) 2 , Ca(OH) 2 , Na 2 CO 3 , ZnCO 3 , Ca 3 (PO 4 ) 2 , ZnCl 2 , (C 2 H 3 ) 2 Zn, CdCl 2 , HgCl 2 , CoCl 2 , (CaNO 3 ) 2 , BaCl 2 , MgCl 2 , PbCl 2 , AlCl 3 , FeCl 2 , FeCl 3 , NiCl 2 , AgCl, AuCl 3 , CuCl 2 , sodium dodecyl sulfate, sodium tetradecyl sulfate, dodecyltrimethylammonium bromide, dodecyltrimethylammonium chloride, tetradecyltrimethylammonium bromide, and a combination thereof.

11. The aqueous two-phase system nanofilter of claim 1 , wherein each of the first aqueous phase and the second aqueous phase has a concentration of 0.001 to 20 wt %.

12. The aqueous two-phase system nanofilter of claim 1 , wherein the aqueous two-phase system nanofilter comprises a channel extending in one direction and having a hollow portion that is opened at an end thereof, and the channel has a body that is mounted in a filter housing to extend in a first direction.

13. The aqueous two-phase system nanofilter of claim 1 , wherein the aqueous two-phase system nanofilter further comprises at least one of a temperature controller or an ultrasonic generator.

Assignees

• Postech Acad Ind Found
• Exosomeplus Inc

Inventors

• Park Jae Sung
• Shin Hyun Woo
• Jeong Hwa Pyeong
• Cho Si Woo

Classifications

• B01D2325/0283

  Pore size · CPC title

• B01D2313/903

  Integrated control or detection device · CPC title

• C02F1/442

  by nanofiltration · CPC title

• B01D2311/10

  Temperature control · CPC title

• B01D2311/02

  Specific process operations before starting the membrane separation process · CPC title