Stable inoculant compositions and methods for producing same

Patent metadata

Field	Value
Publication number	US-12173167-B2
Application number	US-202217830445-A
Country	US
Kind code	B2
Filing date	Jun 2, 2022
Priority date	Sep 11, 2015
Publication date	Dec 24, 2024
Grant date	Dec 24, 2024

Abstract

The present disclosure provides stable inoculant compositions and methods for enhancing the survival and/or stability of microorganisms in an inoculant composition. In some embodiments, the microorganisms in an inoculant compositions are stabilized by the presence of one or more maltodextrins having a dextrose equivalent value of about 15 to about 20.

First claim

That which is claimed:

1. An aqueous inoculant composition comprising:
one or more maltodextrins having a dextrose equivalent value of about 15 to about 20;
one or more disaccharides;
one or more hygroscopic polymers selected from the group consisting of carboxymethyl celluloses, gums and polyvinyl alcohols; and
one or more strains of Gram-negative bacteria, in which one or more of the following is true:
said one or more maltodextrins and said one or more disaccharides are present in a maltodextrin: disaccharide ratio of about 35:65 to about 65:35;
said one or more disaccharides comprises maltose;
said one or more strains of Gram-negative bacteria is/are present in a concentration ranging from about 1×10 4 to about 1×10 11 colony-forming units per gram of said aqueous inoculant composition.

2. The aqueous inoculant composition of claim 1 , in which said one or more maltodextrins and said one or more disaccharides are present in a maltodextrin: disaccharide ratio of about 35:65 to about 65:35.

3. The aqueous inoculant composition of claim 1 , in which said one or more disaccharides comprises maltose.

4. The aqueous inoculant composition of claim 1 , in which said one or more hygroscopic polymers comprises a carboxymethyl cellulose.

5. The aqueous inoculant composition of claim 1 , in which said one or more hygroscopic polymers comprises one or more gums.

6. The aqueous inoculant composition of claim 1 , in which said one or more hygroscopic polymers comprises xantham gum.

7. The aqueous inoculant composition of claim 1 , in which said one or more hygroscopic polymers comprises a polyvinyl alcohol.

8. The aqueous inoculant composition of claim 1 , in which said one or more hygroscopic polymers comprises a carboxymethyl cellulose and a polyvinyl alcohol.

9. The aqueous inoculant composition of claim 1 , in which said one or more hygroscopic polymers comprises about 0.1 to about 5% of said aqueous inoculant composition, by weight, based upon the total weight of said aqueous inoculant composition.

10. The aqueous inoculant composition of claim 1 , in which said one or more hygroscopic polymers comprises about 0.1 to about 1% of said aqueous inoculant composition, by weight, based upon the total weight of said aqueous inoculant composition.

11. The aqueous inoculant composition of claim 1 , in which said one or more strains of Gram-negative bacteria is/are present in a concentration of at least 1×10 5 colony-forming units per gram of said inoculant composition.

12. The aqueous inoculant composition of claim 1 , in which said one or more Gram-negative bacteria is/are present at a concentration of at least 1×10 7 colony-forming units per gram of said inoculant composition.

13. The aqueous inoculant composition of claim 1 , further comprising one or more surfactants.

14. The aqueous inoculant composition of claim 13 , in which said one or more surfactants comprises one or more siloxanes.

15. The aqueous inoculant composition of claim 13 , in which said one or more surfactants comprises one or more trisiloxanes.

16. A method comprising applying an aqueous inoculant composition to a plant or plant part, said aqueous inoculant composition comprising:
one or more maltodextrins having a dextrose equivalent value of about 15 to about 20;
one or more disaccharides;
one or more hygroscopic polymers; and
one or more strains of Gram-negative bacteria.

17. A method comprising introducing an aqueous inoculant composition into a plant growth medium comprising soil, said aqueous inoculant composition comprising:
one or more maltodextrins having a dextrose equivalent value of about 15 to about 20;
one or more disaccharides;
one or more hygroscopic polymers; and
one or more strains of Gram-negative bacteria.

18. A coated plant seed, comprising:
a plant seed; and
a coating that covers at least a portion of an outer surface of said plant seed, said coating comprising an aqueous inoculant composition that comprises:
one or more maltodextrins having a dextrose equivalent value of about 15 to about 20;
one or more disaccharides;
one or more hygroscopic polymers; and
one or more strains of Gram-negative bacteria.

19. A kit comprising a plurality of plant seeds that have been treated with an aqueous inoculant composition that comprises:
one or more maltodextrins having a dextrose equivalent value of about 15 to about 20;
one or more disaccharides;
one or more hygroscopic polymers; and
one or more strains of Gram-negative bacteria.

20. A method of improving the stability of a Gram-negative bacteria in an aqueous composition, said method comprising introducing into said composition:
one or more maltodextrins having a dextrose equivalent value of about 15 to about 20;
one or more disaccharides; and
one or more hygroscopic polymers selected from the group consisting of carboxymethyl celluloses, gums and polyvinyl alcohols, in which one or more of the following is true:
said one or more maltodextrins and said one or more disaccharides are introduced into said composition in a maltodextrin: disaccharide ratio of about 35:65 to about 65:35;
said one or more disaccharides comprises maltose.

Assignees

• Novozymes Bioag As

Inventors

• Kellar Kenneth Edmund
• Kang Yaowei
• Pelligra Claire
• Barnett Emily
• Burklew Caitlin
• Wysinski Anna
• Leland Jarrod E
• Doughan Ben
• Fethe Michael Harrison
• Trahan Ashley Delanie
• Greenshields David
• Woods Kristi

Classifications

• A01N63/20 Primary

  Bacteria; Substances produced thereby or obtained therefrom · CPC title

• A01N43/16

  with oxygen as the ring hetero atom · CPC title

• A01N25/14

  wettable · CPC title

• C09D105/02

  Dextran; Derivatives thereof · CPC title

• A01N25/26

  in coated particulate form · CPC title