Enzymatically polymerized gelling dextrans
US-11390692-B2 · Jul 19, 2022 · US
US12173091B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12173091-B2 |
| Application number | US-202217865904-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 15, 2022 |
| Priority date | Nov 5, 2014 |
| Publication date | Dec 24, 2024 |
| Grant date | Dec 24, 2024 |
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Compositions are disclosed herein comprising dextran that comprises (i) 87-93 wt % glucose linked at positions 1 and 6; (ii) 0.1-1.2 wt % glucose linked at positions 1 and 3; (iii) 0.1-0.7 wt % glucose linked at positions 1 and 4; (iv) 7.7-8.6 wt % glucose linked at positions 1, 3 and 6; and (v) about 0.4-1.7 wt % glucose linked at (a) positions 1, 2 and 6, or (b) positions 1, 4 and 6. Aqueous forms of this composition have enhanced viscosity profiles. Further disclosed are methods of using compositions comprising dextran, such as increasing the viscosity of an aqueous composition. Enzymatic reactions for producing dextran are also disclosed.
Opening claim text (preview).
What is claimed is: 1. An isolated composition comprising water, sucrose and a glucosyltransferase enzyme comprising an amino acid sequence that is at least 98% identical to SEQ ID NO:1 or SEQ ID NO:2. 2. The isolated composition of claim 1 , wherein the glucosyltransferase enzyme comprises the amino acid sequence that is at least 98% identical to SEQ ID NO:1. 3. The isolated composition of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 99% identical to SEQ ID NO:1. 4. The isolated composition of claim 1 , wherein the glucosyltransferase enzyme comprises the amino acid sequence of SEQ ID NO:1. 5. The isolated composition of claim 1 , wherein the glucosyltransferase enzyme comprises the amino acid sequence that is at least 98% identical to SEQ ID NO:2. 6. The isolated composition of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 99% identical to SEQ ID NO:2. 7. The isolated composition of claim 1 , wherein the glucosyltransferase enzyme comprises the amino acid sequence of SEQ ID NO:2. 8. The isolated composition of claim 1 , comprised within an inert vessel. 9. The isolated composition of claim 8 , comprised within a stainless steel vessel, plastic vessel, or glass vessel. 10. The isolated composition of claim 1 , wherein the isolated composition comprises only one type of glucosyltransferase enzyme. 11. The isolated composition of claim 1 , wherein the isolated composition is cell-free. 12. The isolated composition of claim 1 , wherein the isolated composition is a food product. 13. The isolated composition of claim 2 , wherein the isolated composition is a food product. 14. The isolated composition of claim 3 , wherein the isolated composition is a food product. 15. The isolated composition of claim 4 , wherein the isolated composition is a food product. 16. The isolated composition of claim 12 , wherein the food product is a cultured dairy product. 17. The isolated composition of claim 16 , wherein the cultured dairy product is cottage cheese or cheese. 18. The isolated composition of claim 16 , wherein the cultured dairy product is sour cream. 19. The isolated composition of claim 16 , wherein the cultured dairy product is yogurt. 20. The isolated composition of claim 12 , wherein the food product is a frozen dessert or ice cream.
Dextran; Derivatives thereof · CPC title
Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin · CPC title
Dextran · CPC title
produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title
Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran · CPC title
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