Plant regulatory elements and uses thereof

Patent metadata

Field	Value
Publication number	US-12168771-B2
Application number	US-202217819266-A
Country	US
Kind code	B2
Filing date	Aug 11, 2022
Priority date	Aug 17, 2021
Publication date	Dec 17, 2024
Grant date	Dec 17, 2024

Abstract

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules. Also provided are methods of the use of the recombinant DNA molecules and constructs and the transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules and constructs.

First claim

What is claimed is:

1. A recombinant DNA molecule comprising a polynucleotide having a sequence selected from the group consisting of:
a) a sequence with at least 95 percent sequence identity to SEQ ID NO: 1 or 2 and having promoter activity;
b) a sequence comprising SEQ ID NO:1 or 2; and
c) a fragment comprising at least 150 contiguous nucleotides of SEQ ID NO:1 or 2, wherein the fragment has promoter activity;
wherein said polynucleotide is operably linked to a heterologous transcribable DNA molecule.

2. The recombinant DNA molecule of claim 1 , wherein said polynucleotide has at least 97 percent sequence identity to the DNA sequence of SEQ ID NO: 1 or 2 and has promoter activity.

3. The recombinant DNA molecule of claim 1 , wherein said polynucleotide has at least 99 percent sequence identity to the DNA sequence of SEQ ID NO: 1 or 2 and has promoter activity.

4. The recombinant DNA molecule of claim 1 , wherein said polynucleotide comprises promoter activity.

5. The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable DNA molecule comprises a gene of agronomic interest.

6. The recombinant DNA molecule of claim 5 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

7. The recombinant DNA molecule of claim 5 , wherein the gene of agronomic interest confers pest resistance in plants.

8. The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable DNA molecule encodes an dsRNA, an miRNA, or a siRNA.

9. A transgenic plant cell comprising a recombinant DNA molecule comprising a polynucleotide selected from the group consisting of:
a) a sequence with at least 95 percent sequence identity to SEQ ID NO: 1 or 2 and having promoter activity;
b) a sequence comprising SEQ ID NO:1 or 2; and
c) a fragment comprising at least 150 contiguous nucleotides of SEQ ID NO:1 or 2, wherein the fragment has promoter activity;
wherein said polynucleotide is operably linked to a heterologous transcribable DNA molecule.

10. The transgenic plant cell of claim 9 , wherein said transgenic plant cell is a monocotyledonous plant cell.

11. The transgenic plant cell of claim 9 , wherein said transgenic plant cell is a dicotyledonous plant cell.

12. A transgenic plant, or part thereof, comprising the recombinant DNA molecule of claim 1 .

13. A progeny plant of the transgenic plant of claim 12 , or a part thereof, wherein the progeny plant or part thereof comprises said recombinant DNA molecule.

14. A transgenic seed, wherein the seed comprises the recombinant DNA molecule of claim 1 .

15. A method of producing a commodity product comprising obtaining the transgenic plant or part thereof according to claim 12 and producing the commodity product therefrom.

16. The method of claim 15 , wherein the commodity product is selected from the group consisting of seeds, processed seeds, protein concentrate, protein isolate, starch, grains, plant parts, seed oil, biomass, flour and meal.

17. A method of expressing a transcribable DNA molecule comprising obtaining the transgenic plant according to claim 12 and cultivating the plant, wherein the transcribable DNA is expressed.

18. A method of expressing a transcribable DNA molecule in root tissue, comprising:
a) transforming a plant cell with a vector comprising a promoter with at least 95 percent sequence identity to SEQ ID NO: 2 and having promoter activity or an active fragment thereof comprising at least 150 contiguous nucleotides of SEQ ID NO: 2 operably linked to a heterologous transcribable DNA molecule; and
b) inducing the cell to form a plant.

19. The method of claim 18 , wherein the vector further comprises a sequence comprising SEQ ID NO: 3 operably linked to the promoter.

20. The method of claim 18 , wherein the vector further comprises a sequence comprising SEQ ID NO: 4 operably linked to the promoter.

21. The method of claim 18 , wherein the vector further comprises a sequence comprising SEQ ID NO: 6 operably linked to the promoter.

22. The method of claim 18 , wherein the heterologous transcribable DNA molecule comprises a gene of agronomic interest.

23. The method of claim 22 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

24. The method of claim 22 , wherein the gene of agronomic interest confers pest resistance in plants.

25. The method of claim 22 , wherein the heterologous transcribable DNA molecule encodes an dsRNA, an miRNA, or a siRNA.

26. The method of claim 22 , wherein the plant cell is selected from the group consisting of a corn cell, a soybean cell, a cotton cell, a wheat cell, a canola cell, a rice cell, and a tomato cell.

27. The recombinant DNA molecule of claim 1 , wherein said recombinant DNA molecule comprises a polynucleotide having a sequence with at least 95 percent sequence identity to SEQ ID NO: 1 or 2 and having promoter activity.

28. The recombinant DNA molecule of claim 1 , wherein said recombinant DNA molecule comprises a polynucleotide having a sequence comprising SEQ ID NO:1 or 2.

29. The recombinant DNA molecule of claim 1 , wherein said recombinant DNA molecule comprises a polynucleotide having a fragment comprising at least 150 contiguous nucleotides of SEQ ID NO:1 or 2, and wherein the fragment has promoter activity.

30. The transgenic plant cell of claim 9 , wherein said recombinant DNA molecule comprises a polynucleotide having a sequence with at least 95 percent sequence identity to SEQ ID NO: 1 or 2 and having promoter activity.

31. The transgenic plant cell of claim 9 , wherein said recombinant DNA molecule comprises a polynucleotide having a sequence comprising SEQ ID NO:1 or 2.

32. The transgenic plant cell of claim 9 , wherein said recombinant DNA molecule comprises a polynucleotide having a fragment comprising at least 150 contiguous nucleotides of SEQ ID NO:1 or 2, and wherein the fragment has promoter activity.

Assignees

• Monsanto Technology Llc

Inventors

• Chittoor Jaishree M
• Flasinski Stanislaw

Classifications

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12N15/8274 Primary

  for herbicide resistance · CPC title

• C12N15/8227 Primary

  Root-specific · CPC title

• C12N15/8229

  Meristem-specific, e.g. nodal, apical · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title