Pseudomonas strains and their metabolites to control plant diseases

Patent metadata

Field	Value
Publication number	US-12167733-B2
Application number	US-202318103082-A
Country	US
Kind code	B2
Filing date	Jan 30, 2023
Priority date	Oct 5, 2020
Publication date	Dec 17, 2024
Grant date	Dec 17, 2024

Abstract

The present disclosure concerns methods of using novel bacterial strains of 0617-T307, 0917-T305, 0917-T306, 0917-T307, 0118-T319, 0318-T327, and 0418-T328, the cell broth and novel metabolites produced from the bacterial strains, that can inhibit the growth of a variety of microbial species for a variety of crops. The methods include use of novel, potent antimicrobial metabolites produced from the strains corresponding to compounds having Formulas (I), (II), and (III):

First claim

What is claimed is:

1. A method of controlling a bacterial crop disease, comprising the steps of
(i) producing an agricultural composition comprising an agriculture composition comprising Formula (I):

and
(ii) applying said agricultural composition to a crop to inhibit the growth of a pathogenic microorganism in the crop.

2. The method of claim 1 , wherein the crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.

3. The method of claim 1 , wherein the pathogenic microorganism is selected from the group consisting of Mycosphaerella fijiensis, Botrytis cinereal, Erwinia amylovora, Xanthomonas axonopodis pv. citri, Pectobacterium parmentieri, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. brasiliensis, Pectobacterium carotovorum subsp. carotovorum, Dickeya dadantii, Pseudomonas savastanoi pv. savastanoi, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv syringae, Pseudomonas syringae pv. lachrymans, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. vesicatoria, Xanthomonas arboricola pv. juglandis, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, Phytophthora infestans, Venturia inaequalis, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola and Xanthomonas citri pv. citri.

4. The method according to claim 1 , wherein the crop is selected from the group consisting of bananas, citrus, carrots, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae, a stone fruit, and a pome fruit.

5. The method according to claim 2 , wherein the crop is selected from the group consisting of bananas, carrots, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae, a stone fruit, and a pome fruit.

6. The method according to claim 3 , wherein the crop is selected from the group consisting of bananas, citrus, carrots, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae, a stone fruit, and a pome fruit.

7. A method of controlling a bacterial crop disease, comprising the steps of
producing an agricultural composition comprising Formula (II):

and
applying said agricultural composition to a crop to inhibit the growth of a pathogenic microorganism in the crop.

8. The method of claim 7 , wherein the crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.

9. The method of claim 7 , wherein the pathogenic microorganism is selected from the group consisting of Mycosphaerella fijiensis, Botrytis cinereal, Erwinia amylovora, Xanthomonas axonopodis pv. citri, Pectobacterium parmentieri, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. brasiliensis, Pectobacterium carotovorum subsp. carotovorum, Dickeya dadantii, Pseudomonas savastanoi pv. savastanoi, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv syringae, Pseudomonas syringae pv. lachrymans, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. vesicatoria, Xanthomonas arboricola pv. juglandis, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, Phytophthora infestans, Venturia inaequalis, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola and Xanthomonas citri pv. citri.

10. The method according to claim 7 , wherein the crop is selected from the group consisting of bananas, citrus, carrots, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae, a stone fruit, and a pome fruit.

11. The method according to claim 8 , wherein the crop is selected from the group consisting of bananas, citrus, carrots, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae, a stone fruit, and a pome fruit.

12. The method according to claim 9 , wherein the crop is selected from the group consisting of bananas, citrus, carrots, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae, a stone fruit, and a pome fruit.

13. A method of controlling a bacterial crop disease, comprising the steps of
(i) producing an agricultural composition comprising Formula (III):

and
(ii) applying said agricultural composition to a crop to inhibit the growth of a pathogenic microorganism in the crop.

14. The method of claim 13 , wherein the crop disease is selected from the group consisting of Black sigatoka, Grey mould, Fire blight, Citrus canker, soft rot, Olive knot, Tomato bacterial speck, Bacterial canker or blast, Angular Leaf Spot of Cucurbits, Bacterial Spot of Peach, Tomato bacterial spot, walnut blight, bacterial wilt, Tomato canker, Potato late blight, apple scab, bacterial leaf blight, and bacterial leaf streak.

15. The method of claim 13 , wherein the pathogenic microorganism is selected from the group consisting of Mycosphaerella fijiensis, Botrytis cinereal, Erwinia amylovora, Xanthomonas axonopodis pv. citri, Pectobacterium parmentieri, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. brasiliensis, Pectobacterium carotovorum subsp. carotovorum, Dickeya dadantii, Pseudomonas savastanoi pv. savastanoi, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv syringae, Pseudomonas syringae pv. lachrymans, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. vesicatoria, Xanthomonas arboricola pv. juglandis, Ralstonia solanacearum, Clavibacter michiganensis subsp. michiganensis, Phytophthora infestans, Venturia inaequalis, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola and Xanthomonas citri pv. citri.

16. The method according to claim 13 , wherein the crop is selected from the group consisting of bananas, citrus, carrots, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae, a stone fruit, and a pome fruit.

17. The method according to claim 14 , wherein the crop is selected from the group consisting of bananas, citrus, carrots, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae, a stone fruit, and a pome fruit.

18. The method according to claim 15 , wherein the crop is selected from the group consisting of bananas, citrus, carrots, onions, rice, African violets, plant species of Cruciferae , plant species of Solanaceae , plant species of Cucurbitaceae, a stone fruit, and a pome

Assignees

• T3 Bioscience Inc
• Uwm Res Foundation Inc

Inventors

• Yang Ching-Hong
• Liu Xiangyang

Classifications

• A61K35/74

  Bacteria (therapeutic use of a bacterial protein A61K38/00) · CPC title

• C07D491/048

  the oxygen-containing ring being five-membered · CPC title

• C07D213/90 Primary

  having more than three double bonds between ring members or between ring members and non-ring members · CPC title

• C07B2200/13

  Crystalline forms, e.g. polymorphs · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title