Cell sorter with pivotal scuppers

Patent metadata

Field	Value
Publication number	US-12162013-B2
Application number	US-202217829321-A
Country	US
Kind code	B2
Filing date	May 31, 2022
Priority date	Feb 5, 2021
Publication date	Dec 10, 2024
Grant date	Dec 10, 2024

Abstract

A compact sorting flow cytometer system is disclosed. The system includes a flow cell and a deflection unit in communication with the flow cell to receive charged drops and uncharged drops in a stream of a sample biological fluid. The deflection unit includes a case having a deflection cone and chamber, a tub with a drain, and a slot in a base. The deflection unit further includes electrostatic charge plates in the cone forming an electrostatic charge field; and first and second pivotal scuppers, and a center non-pivotal collector arranged along a shaft in the tub of the case. Uncharged drops pass the electrostatic charge field undeflected in a center stream path while charged drops are deflected away from it. The pivotal scuppers pivot between positions to direct charged drops into the tub for aspiration or to pass through the slot in the base for collection.

First claim

What is claimed is:

1. A compact sorting flow cytometer system comprising:
a flow cell to form a stream of a plurality of drops of a sample biological fluid with one or more biological cells or particles, the flow cell having a flow cell body with a port receiving a conductive hose fitting to receive a signal from a sort controller to selectively apply a charge to a sheath fluid and at least one drop of the sample biological fluid to form at least one charged drop and otherwise leave a plurality of uncharged drops;
a deflection unit in communication with the flow cell to receive the at least one charged drop and the plurality of uncharged drops in the stream of the sample biological fluid, the deflection unit including:
a case having a deflection cone and a deflection chamber in communication with the flow cell to receive the at least one charged drop and the plurality of uncharged drops in the stream, the case further having a tub with a drain in the deflection chamber to aspirate drops into a waste outlet, the case further having a slot at a base;
a first electrostatic charge plate and a second electrostatic charge plate opposite the first electrostatic charge plate mounted in the deflection cone with a progressively larger gap between each, the first charge plate to receive a high positive voltage and the second charge plate to receive a high negative voltage to form an electrostatic charge field through which the at least one charged drop and the plurality of uncharged drops in the stream pass, wherein the plurality of uncharged drops pass through the electrostatic charge field in a center stream path of drops of the sample biological fluid and the at least one charged drop is deflected away from the center stream path; and
a first pivotal scupper, a second pivotal scupper, and a non-pivotal center collector between the first pivotal scupper and the second pivotal scupper are arranged along a first shaft in the tub of the case, wherein the first pivotal scupper and the second pivotal scupper pivot with the first shaft between a first position to direct drops into the tub for aspiration down the drain and a second position to allow the at least one charged drop deflected away from the center stream path to pass through the slot for collection, wherein the non-pivotal center collector is non-pivotal to direct the plurality of uncharged drops in the center stream path into the tub for aspiration down the drain.

2. The compact sorting flow cytometer system of claim 1 , wherein
the first pivotal scupper, the second pivotal scupper, and the non-pivotal center collector are horizontally adjustable (side to side) with the first shaft to align the non-pivotal center collector with the center stream path of the plurality of uncharged drops from the flow cell.

3. The compact sorting flow cytometer system of claim 1 , further comprising:
a second shaft inserted through a hollow opening in the first shaft; and
a threaded adjustment knob engaged with threads of the second shaft to pull on the second shaft as turned in one direction and push out on the second shaft as turned in the opposite direction to adjust the horizontal position of the first pivotal scupper, the second pivotal scupper, and the non-pivotal center collector.

4. The compact sorting flow cytometer system of claim 3 , wherein
the deflection unit is slidingly mounted to at least one rail and horizontally adjustable along the at least one rail to adjust a position of the center stream path.

5. The compact sorting flow cytometer system of claim 4 , wherein
the deflection unit is horizontally adjusted to adjust the position of the center stream path to be equidistant between the first electrostatic charge plate and the second electrostatic charge plate in the deflection cone.

6. The compact sorting flow cytometer system of claim 3 , further comprising:
a first pulley coupled to the first shaft;
a reversible electric motor with a second pulley coupled to a third shaft, the third shaft rotated clockwise and counter clockwise by the reversible electric motor;
a continuous belt mounted over the first pulley and the second pulley;
wherein the continuous belt drives the first pulley in response to the reversable electric motor to pivot the first pivotal scupper and the second pivotal scupper pivot between the first position and the second position.

7. The compact sorting flow cytometer system of claim 1 , wherein
the deflection unit is slidingly mounted to at least one rail and horizontally adjustable along the at least one rail to adjust a position of the center stream path.

8. The compact sorting flow cytometer system of claim 7 , wherein
the deflection unit is horizontally adjusted to adjust the position of the center stream path to be equidistant between the first electrostatic charge plate and the second electrostatic charge plate in the deflection cone.

9. The compact sorting flow cytometer system of claim 1 , wherein the signal is one of
a ground signal apply ground to the sheath fluid and leave the plurality of uncharged drops uncharged;
a positive charge signal to apply a positive charge to the sheath fluid that is transferred to a drop to form a positively charged drop; and
a negative charge signal to apply a negative charge to the sheath fluid that is transferred to a drop to form a negatively charged drop.

Assignees

• Cytek Biosciences Inc

Inventors

• Krueger Glen
• Vrane David
• Wang Kuncheng
• Gu Qiuta

Classifications

• G01N2015/1418

  Eliminating clogging of debris · CPC title

• C12Q2565/626

  being a flow cytometer · CPC title

• G01N33/4915

  using flow cells (flow cytometry G01N15/14) · CPC title

• G01N35/1095

  for supplying the samples to flow-through analysers (for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00) · CPC title

• G01N15/1484

  microstructural devices · CPC title