Rotatable device containing wafers for DNA processing

Patent metadata

Field	Value
Publication number	US-12157102-B2
Application number	US-202217863033-A
Country	US
Kind code	B2
Filing date	Jul 12, 2022
Priority date	May 22, 2018
Publication date	Dec 3, 2024
Grant date	Dec 3, 2024

Abstract

A system includes a synthesizer unit having a fluid input to receive fluids and a communication input to receive commands to synthesize data-encoded DNA sequences and cleave the DNA. A first flexible chemistry reaction chamber module may be fluidically coupled to the synthesizer unit to receive the data-encoded DNA sequences and amplify the sequences. A deposition unit may be fluidically coupled to the first flexible chemistry reaction chamber module to receive the amplified DNA sequences and encapsulate the amplified DNA sequences into one or more wells in a storage plate for storage and retrieval to and from a plate storage unit. Retrieved DNA may be processed and read by further units.

First claim

The invention claimed is:

1. A device comprising:
a chamber configured to rotate about a central axis;
a plurality of wafers stacked within the chamber and coaxially aligned with the central axis of the chamber;
a rotating component of a motor device coupled to the plurality of wafers and configured to rotate the plurality of wafers;
a plurality of DNA processing sites disposed on a surface of each wafer of the plurality of wafers to process DNA, wherein the device is configured to remove a reagent from the plurality of wafers by centrifugal force caused by rotation of the plurality of wafers about the central axis; and
a controller communicatively coupled to the motor and programmed to cause the rotation of the plurality of wafers at a revolutions per minute (rpm) sufficient to create the centrifugal force to remove the reagent.

2. The device of claim 1 , wherein the plurality of wafers is formed of silicon.

3. The device of claim 1 , wherein each of the plurality of wafers has a central opening.

4. The device of claim 3 , wherein the DNA processing sites on each wafer of the plurality of wafers are arranged in a pattern extending radially from the central opening toward a respective outer edge of each wafer.

5. The device of claim 3 , wherein each of the DNA processing sites are a same size and are arranged in concentric circles of reticles about the central opening.

6. The device of claim 3 , wherein the rotating component is configured to couple to the plurality of wafers through the central opening and rotate the plurality of wafers about the central axis.

7. The device of claim 1 , wherein the rotating component of the motor device comprises a rotary servo drive.

8. The device of claim 1 , wherein a wafer of the plurality of wafers has a central region and the device further comprising a circular gasket disposed between the central region and the DNA processing sites.

9. The device of claim 8 , wherein the DNA processing sites are arranged in a pattern extending radially from the circular gasket toward an outer edge of the wafer.

10. The device of claim 8 , further comprising electronics on the wafer and positioned in the central region within the circular gasket.

11. The device of claim 10 , further comprising a flex circuit coupled to the electronics on the wafer.

12. The device of claim 1 , further comprising a plurality of standoff wafer pitch fiducials formed between the DNA processing sites in concentric circles around radial edges of the DNA processing sites, wherein the plurality of standoff wafer pitch fiducials serve as visual markers for detecting positions of the DNA processing sites on the plurality of wafers.

13. The device of claim 1 , further comprising a fluid inlet configured to introduce fluids into the chamber.

14. The device of claim 1 , further comprising a fluid exit opening configured to remove fluids from the chamber.

15. The device of claim 1 , wherein the DNA processing sites are configured to hold fluid during DNA processing and provide a substrate for DNA synthesis.

16. The device of claim 15 , wherein the DNA processing sites further comprise reading mechanisms through which DNA is drawn in response to an applied voltage.

17. The device of claim 1 , further comprising a vacuum source configured to create a vacuum within the chamber to add a reagent to the chamber.

18. The device of claim 1 , further comprising a circular gasket configured to provide a seal between adjacently mounted wafers.

Assignees

• Microsoft Technology Licensing Llc

Inventors

• Nguyen Bichlien H
• Kelley Douglas P
• Strauss Karin
• Carlson Robert
• Parker Hsing-Yeh
• Mulligan John
• Ceze Luis H
• Chen Yuan-Jyue
• Carmean Douglas

Classifications

• B01J2219/00722

  Nucleotides · CPC title

• B01J2219/0068

  Means for controlling the apparatus of the process · CPC title

• B01J2219/00277

  Apparatus · CPC title

• G11C13/0019

  comprising bio-molecules · CPC title

• Y02P20/582

  Recycling of unreacted starting or intermediate materials · CPC title