Functionalized plasmonic nanostructures

Patent metadata

Field	Value
Publication number	US-12152237-B2
Application number	US-202117525553-A
Country	US
Kind code	B2
Filing date	Nov 12, 2021
Priority date	Nov 16, 2020
Publication date	Nov 26, 2024
Grant date	Nov 26, 2024

Abstract

An example of a functionalized plasmonic nanostructure includes a plasmonic nanostructure core; a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm; and a plurality of primers attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core.

First claim

What is claimed is:

1. A functionalized plasmonic nanostructure, comprising:
a plasmonic nanostructure core;
a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm;
a plurality of two primers that together seed and amplify a template nucleic acid strand, the plurality of two primers being attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core; and
a mechanism to anchor the functionalized plasmonic nanostructure to a capture site of a flow cell.

2. The functionalized plasmonic nanostructure as defined in claim 1 , wherein the plasmonic nanostructure core is selected from the group consisting of a gold nanostructure, a silver nanostructure, a tin nanostructure, a rhodium nanostructure, a ruthenium nanostructure, a palladium nanostructure, an osmium nanostructure, an iridium nanostructure, a platinum nanostructure, a chromium nanostructure, a copper nanostructure, a gallium arsenide nanostructure, a doped silicon nanostructure, an aluminum nanostructure, a magnesium nanostructure, a silver and gold composite nanostructure, and combinations thereof.

3. The functionalized plasmonic nanostructure as defined in claim 1 , wherein:
the polymeric hydrogel includes at least one acrylamide monomer unit; and
the polymeric hydrogel is a linear polymeric hydrogel or a branched polymeric hydrogel.

4. The functionalized plasmonic nanostructure as defined in claim 1 , wherein:
the plasmonic nanostructure core is functionalized with an alkyne, and the polymeric hydrogel includes an azide that is attached to the alkyne; or
the plasmonic nanostructure core is functionalized with an azide, and the polymeric hydrogel includes a dialkyne that is attached to the azide.

5. The functionalized plasmonic nanostructure as defined in claim 1 , wherein the mechanism is a magnetic material in the plasmonic nanostructure core.

6. The functionalized plasmonic nanostructure as defined in claim 1 , wherein the mechanism is a functional agent incorporated into the polymeric hydrogel, wherein the functional agent is selected from the group consisting of a target nucleic acid that is complementary to a capture nucleic acid on a flow cell and a member of a binding pair that is capable of binding to a second member of a binding pair that is attached to the flow cell.

7. The functionalized plasmonic nanostructure as defined in claim 1 , wherein the mechanism is a reversibly chargeable functional group attached to the polymeric hydrogel.

8. The functionalized plasmonic nanostructure as defined in claim 1 , wherein the different distances range from greater than 0 nm to about 20 nm.

9. A kit, comprising:
a flow cell including a plurality of capture sites, wherein each capture site is a magnetic capture site, or includes a chemical capture agent, or includes an electrostatic agent; and
a suspension including:
a liquid carrier; and
a plurality of functionalized plasmonic nanostructures dispersed throughout the liquid carrier, wherein each of the functionalized plasmonic nanostructures includes:
a plasmonic nanostructure core;
a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm;
a plurality of two primers that together seed and amplify a template nucleic acid strand, the plurality of two primers being attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core; and
a mechanism to attach to the capture site of the flow cell.

10. The kit as defined in claim 9 , wherein the plasmonic nanostructure core is selected from the group consisting of a gold nanostructure, a silver nanostructure, a tin nanostructure, a rhodium nanostructure, a ruthenium nanostructure, a palladium nanostructure, an osmium nanostructure, an iridium nanostructure, a platinum nanostructure, a chromium nanostructure, a copper nanostructure, a gallium arsenide nanostructure, a doped silicon nanostructure, an aluminum nanostructure, a magnesium nanostructure, a silver and gold composite nanostructure, and combinations thereof.

11. The kit as defined in claim 9 , wherein:
the polymeric hydrogel includes at least one acrylamide monomer unit; and
the polymeric hydrogel is a linear polymeric hydrogel or a branched polymeric hydrogel.

12. The kit as defined in claim 9 , wherein:
each of the plurality of capture sites is the magnetic capture site; and
the mechanism is a magnetic material included in the plasmonic nanostructure core.

13. The kit as defined in claim 9 , wherein:
each of the plurality of capture sites includes the chemical capture agent;
the mechanism is a functional agent incorporated into the polymeric hydrogel; and
one of:
the chemical capture agent is a capture oligonucleotide and the mechanism is a target nucleic acid that is complementary to the capture oligonucleotide; or
the mechanism is a member of a binding pair and the chemical capture agent is a second member of the binding pair.

14. The kit as defined in claim 9 , wherein:
each of the plurality of capture sites includes the electrostatic capture agent; and
the mechanism is a reversibly chargeable functional group attached to the polymeric hydrogel.

15. The kit as defined in claim 9 , wherein the different distances range from greater than 0 nm to about 20 nm.

16. The kit as defined in claim 9 , wherein:
the flow cell further includes a substrate that supports the plurality of capture sites;
the substrate includes depressions separated by interstitial regions; and
at least one of the plurality of capture sites is positioned in each of the depressions.

17. The kit as defined in claim 9 , wherein:
the flow cell further includes a substrate that supports the plurality of capture sites;
the substrate includes posts separated by interstitial regions; and
at least one of the plurality of capture sites is positioned over each of the posts.

18. The kit as defined in claim 9 , wherein:
the flow cell further includes a substrate that supports the plurality of capture sites;
the substrate has a substantially flat surface; and
the plurality of capture sites is positioned in a pattern across the substantially flat surface.

19. A flow cell, comprising:
a substrate including a plurality of capture sites, wherein each capture site is a magnetic capture site, or includes a chemical capture agent, or includes an electrostatic agent; and
functionalized plasmonic nanostructures anchored to at least some of the plurality of capture sites, each functionalized plasmonic nanostructure including:
a plasmonic nanostructure core;
a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm;
a plurality of two primers that together seed and amplify a template nucleic acid strand, the plurality of two primers being attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core; and
a mechanism anchoring the functionalized plasmonic nanostructure to the capture site.

20. Th

Assignees

• Illumina Inc
• Illumina Cambridge Ltd

Inventors

• Artioli Gianluca Andrea
• Lessard-Viger Mathieu
• Mather Brian D
• Von Hatten Xavier

Classifications

• C08J2333/26

  Homopolymers or copolymers of acrylamide or methacrylamide · CPC title

• C08J3/075

  Macromolecular gels · CPC title

• C08F265/10

  on to polymers of amides or imides · CPC title

• G01N21/648 Primary

  using evanescent coupling or surface plasmon coupling for the excitation of fluorescence · CPC title

• C12Q1/6869

  Methods for sequencing · CPC title