Multifunctional therapeutic biological material and preparation method thereof

Patent metadata

Field	Value
Publication number	US-12150992-B2
Application number	US-202117492060-A
Country	US
Kind code	B2
Filing date	Oct 1, 2021
Priority date	Apr 6, 2021
Publication date	Nov 26, 2024
Grant date	Nov 26, 2024

Abstract

The present disclosure relates to a multifunctional therapeutic biological material and preparation method thereof. The process steps of the preparation method are as follows: (1) preparing various three-dimensional micro/nano composite structure on the surface of a biomedical material by using femtosecond laser double pulses; (2) further preparing a nano-flower structure on the three-dimensional micro/nano composite structure by using hydrothermal synthesis method to construct a heterogeneous structure consisting of the three-dimensional micro/nano composite structure and the nano-flower structure; (3) putting the prepared heterogeneous structure into a mixed solution containing gold ions and platinum ions, and by using ultraviolet light reduction method, reducing in situ into gold-platinum bimetallic nanoparticles on the heterogeneous structure to obtain the multifunctional therapeutic biological material. The multifunctional therapeutic biological material provided by the present disclosure has excellent light-to-heat conversion characteristics, and it can promote bone regeneration, and has functions of tumor treatment and anti-bacterial infection.

First claim

The invention claimed is:

1. A method for preparing a multifunctional therapeutic biological material, comprising:
step 1: performing mirror polishing pretreatment on a surface of a biomedical material to be processed, ultrasonically cleaning it and drying it, to obtain a clean surface polishing material;
step 2: preparing a large-area and consistent three-dimensional micro/nano composite structure on the surface of the surface polishing material obtained in step 1 by using femtosecond laser, cleaning the processed material with deionized water and drying it;
step 3: preparing a nano-flower structure on the surface of the material obtained in step 2, and constructing a heterogeneous structure consisting of the three-dimensional micro/nano composite structure and the nano-flower structure; and
step 4: putting the heterogeneous structure prepared in step 3 into a mixed solution containing gold ions and platinum ions, and by using ultraviolet light reduction method, reducing in situ into gold-platinum bimetallic nanoparticles on the heterogeneous structure to obtain the multifunctional therapeutic biological material.

2. The method according to claim 1 , wherein the biomedical material in step 1 comprises a medical metal material, medical ceramic material, medical polymer material, and medical non-metal material.

3. The method according to claim 1 , wherein the specific implementation steps of step 2 comprise:
(1) fixing the clean surface polishing material on a glass slide, and fixing the glass slide on a high-precision six-degree-of-freedom translation stage;
(2) modulating the traditional femtosecond laser into femtosecond laser double pulses in the time domain through splitting and combining or pulse shaping, with a time interval between two sub-pulses of 100 fs-100 ps and an energy ratio range of two sub-pulses of 0.1-10;
(3) focusing the generated femtosecond laser double pulses on the surface of the surface polishing material by using an objective lens, and making sample move relative to the laser by controlling the high-precision six-degree-of-freedom translation stage through a computer control system; during processing, using high-pressure nitrogen for blowing chips and controlling laser flux, processing speed, processing distance, and double pulse delay time to prepare the three-dimensional micro/nano composite structure; and
(4) cleaning the material after the above processing with deionized water and drying it.

4. The method according to claim 3 , wherein the specific implementation steps of step 3 comprise:
(1) preparing a mixed solution consisting of sodium hydroxide solution and hydrogen peroxide solution as a hydrothermal reaction system;
(2) putting the biomedical material with a surface of the three-dimensional micro/nano composite structure prepared by the femtosecond laser double pulses processing into a bottom of a reactor, and then pouring the prepared mixed solution into the reactor for hydrothermal reaction; and
(3) cleaning the material after the hydrothermal reaction with deionized water and drying it.

5. The method according to claim 4 , wherein the specific implementation steps of step 4 comprise:
(1) preparing a mixed solution consisting of chloroauric acid and chloroplatinic acid as a precursor solution;
(2) putting the biomedical material with a surface of the heterogeneous structure consisting of the three-dimensional micro/nano composite structure and the nano-flower structure prepared by the femtosecond laser double pulses processing combined with hydrothermal treatment into the bottom of a beaker, then adding the prepared precursor solution dropwise into the beaker, and finally placing an ultraviolet light source horizontally above the beaker for irradiation; and
(3) cleaning the material after photoreduction reaction with deionized water and drying it.

6. The method according to claim 3 , wherein the multiple of the objective lens, laser flux, processing speed, processing distance, and double pulse delay time in step (3) are 5 times, 10 times or 20 times, 1-150 J/cm 2 , 10-2000 μm/s, 2-30 μm and 5-20 ps respectively.

7. The method according to claim 4 , wherein the concentration of the sodium hydroxide solution in step (1) is 1-20 mM, and the concentration of the hydrogen peroxide solution is 5%-30%.

8. The method according to claim 4 , wherein the temperature of the hydrothermal reaction in step (2) is 50-120° C., and the reaction time is 24-48 h.

9. The method according to claim 5 , wherein a concentrations of the chloroauric acid and the chloroplatinic acid in step (1) are both 10-50 mM, and the two solutions are mixed at 1:1 as the precursor solution; and the power of the ultraviolet light source in step (2) is 5-20 W, and the photoreduction process time is 10-30 s.

10. A method for treating tumors and bacterial infections and repairing bone defects, comprising administering to a subject in need thereof the multifunctional therapeutic biological material prepared by the method of claim 1 .

11. A method for treating bone tumors and postoperative bacterial infections, comprising administering to a subject in need thereof the multifunctional therapeutic biological material prepared by the method of claim 1 .

Assignees

• Beijing Institute Tech

Inventors

• Jiang Lan
• Ma Yunlong
• Hu Jie

Classifications

• C23C18/42

  Coating with noble metals · CPC title

• B82Y40/00

  Manufacture or treatment of nanostructures · CPC title

• B82Y30/00

  Nanotechnology for materials or surface science, e.g. nanocomposites · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• A61L2430/02

  for reconstruction of bones; weight-bearing implants · CPC title