Fertility restoration in plants

Patent metadata

Field	Value
Publication number	US-12146148-B2
Application number	US-201917275111-A
Country	US
Kind code	B2
Filing date	Sep 13, 2019
Priority date	Sep 14, 2018
Publication date	Nov 19, 2024
Grant date	Nov 19, 2024

Abstract

Provided herein are methods and compositions for restoring fertility and maintaining sterility in plants. In particular, one method disclosed includes introducing into a male-sterile plant, wherein the plant comprises one or more homozygous mutations in a male-fertility gene, a plant restoration donor chromosomal component comprising a plant-derived polynucleotide that confers a plant phenotypic marker linked to a male-fertility restoration locus that functionally complements the male-sterility phenotype from the one or more homozygous mutations in the male-sterile plant. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker and the male-fertility restoration locus are linked to each other and located on the same chromosomal arm on the plant restoration donor chromosomal component. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker, the male-fertility restoration locus, or both are modified.

First claim

That which is claimed:

1. A method of restoring male fertility in a Ms45 male-sterile wheat plant, the method comprising:
(a) introducing into the Ms45 male-sterile wheat plant, wherein the Ms45 male-sterile wheat plant comprises triple homozygous mutations of a Ms45 male-fertility polynucleotide that cause a male sterility phenotype, a plant restoration donor chromosomal component comprising a 4E chromosomal component from Thinopyrum or Agropyron , the 4E chromosomal component comprising:
a plant polynucleotide that confers a plant seed phenotype linked to a Ms45 male-fertility restoration locus; and

(b) restoring male-fertility to the Ms45 male-sterile wheat plant by the 4E chromosomal component, wherein expression of the 4E donor chromosomal component functionally complements the male-sterility phenotype from the triple homozygous Ms45 mutations in the Ms45 male-sterile wheat plant so that the Ms45 male-sterile wheat plant becomes male-fertile.

2. The method of claim 1 , wherein expression of the Ms45male-fertility restoration locus functionally complements the male-sterility phenotype from the triple homozygous Ms45 mutations in the male-sterile wheat plant.

3. The method of claim 1 , wherein the plant polynucleotide that confers the plant phenotype is located on the same chromosomal arm of the 4E chromosomal component as the Ms45 male-fertility restoration locus and not separated by a centromere.

4. The method of claim 1 , comprising introducing the 4E chromosomal component by crossing the Ms45 male-sterile wheat plant with a male-fertile plant comprising the 4E chromosomal component.

5. The method of claim 4 , wherein the 4E chromosomal component restores fertility to the plant without recombining with the wheat chromosomes.

6. The method of claim 4 , wherein the male-fertile plant is a wheat, barley, oat, wheatgrass, or rye plant or a related species thereof.

7. The method of claim 1 , wherein the male-fertile plant is Sebesta Blue, Blue Sando, Blue Baart, Blue Onas, Blue 1, PBB, or Blue Norco, and wherein the donor chromosomal component is derived from a non-wheat plant species.

8. The method of claim 1 , wherein the plant seed phenotype is a phenotype for color, physiology, or morphology of the seed.

9. The method of claim 8 , wherein the color is blue aleurone, P gene, anthocyanin, or Kala 4.

Assignees

• Pioneer Hi Bred Int

Inventors

• Albertsen Marc C
• Fox Tim
• Singh Manjit
• Williams Mark E

Classifications

• C07K14/415

  from plants · CPC title

• C12N15/90

  Stable introduction of foreign DNA into chromosome · CPC title

• C12N15/8289 Primary

  Male sterility · CPC title

• C12N15/8213 Primary

  Targeted insertion of genes into the plant genome by homologous recombination · CPC title

• A01H5/10 Primary

  Seeds · CPC title