Electrophoretic mass spectrometry probes and systems and uses thereof

Patent metadata

Field	Value
Publication number	US-12142472-B2
Application number	US-202318511114-A
Country	US
Kind code	B2
Filing date	Nov 16, 2023
Priority date	Jul 14, 2017
Publication date	Nov 12, 2024
Grant date	Nov 12, 2024

Abstract

The invention generally relates to electrophoretic mass spectrometry probes and systems and methods of uses thereof. In certain aspects, the invention provides a mass spectrometry probe having a hollow body with a distal tip, an electrically conductive hollow conduit, and an electrode. The electrically conductive hollow conduit may be operably coupled to a reservoir and a power source, and the electrically conductive hollow conduit may be configured to transport a liquid sample into the hollow body and polarize the liquid sample as it flows through the electrically conductive hollow conduit and into in the hollow body. The electrode and the electrically conductive hollow conduit are disposed within the hollow body (e.g., at different heights within the hollow body).

First claim

What is claimed is:

1. A method for analyzing a sample, the method comprising:
providing a liquid sample reservoir that is coupled to a first electrode;
providing a power source coupled to the first electrode;
providing a mass spectrometry probe comprising: a hollow body comprising a distal tip; and a second electrode, wherein the second electrode is disposed within the hollow body;
providing an electrically conductive hollow conduit that is coupled at a first end to the liquid sample reservoir, wherein the electrically conductive conduit has a length such that it extends into the hollow body of the mass spectrometry probe;
introducing a liquid sample comprising a small molecule and a plurality of liquid-phase ions into the liquid sample reservoir;
causing the liquid sample to be moved from the liquid sample reservoir and into the hollow body via the hollow conduit such that a gap is maintained between the liquid sample and the second electrode;
applying a positive voltage to the hollow conduit via voltage from the first electrode being applied to the liquid sample reservoir needle which operably applies the voltage to the hollow conduit, while transporting the liquid sample from the liquid sample reservoir through the hollow conduit and into the hollow body, wherein the liquid sample is polarized as the liquid sample flows through the hollow conduit and into in the hollow body;
inductively applying a negative voltage from the second electrode across the gap to the liquid sample, thereby causing the plurality of liquid-phase ions to separate from the small molecule within the hollow body but not within the hollow conduit;
terminating the negative voltage from the second electrode while maintaining the positive voltage to the hollow conduit to thereby cause the small molecule to be emitted from the distal tip and become ionized; and
analyzing the ionized small molecule in a mass spectrometer.

2. The method of claim 1 , wherein there is an offset of at least-1 V between the first and second electrode during operation of the first and second electrode.

3. The method of claim 1 , wherein the voltage is applied to the first and second electrode for at least 10 seconds.

4. The method of claim 1 , wherein the voltage is applied to the first and second electrode for at least 15 minutes.

5. The method of claim 1 , wherein multiple cycles of voltage application to the first and second electrode is performed.

6. The method of claim 1 , wherein the liquid sample reservoir is a syringe that comprises a needle.

7. The method of claim 6 , wherein the first electrode is coupled to the needle.

8. The method of claim 1 , wherein the liquid sample is a biological sample.

9. The method of claim 8 , wherein the biological sample is blood or urine.

10. The method of claim 1 , wherein applying voltage from the electrode causes the plurality of liquid-phase ions to migrate toward the electrode.

Assignees

• Purdue Research Foundation
• Amgen Inc

Inventors

• Cooks Robert Graham
• Flick Tawnya

Classifications

• H01J49/165

  Electrospray ionisation · CPC title

• G01N27/4473 Primary

  by electric means · CPC title

• G01N30/7266

  by electric field, e.g. electrospray · CPC title

• G01N27/44717

  Arrangements for investigating the separated zones, e.g. localising zones · CPC title

• H01J49/0431 Primary

  for liquid samples (interfaces to liquid chromatographs G01N30/7233) · CPC title