Axial dispersion bioreactor (ADBR) for production of microalgae and other microorganisms

Patent metadata

Field	Value
Publication number	US-12139698-B2
Application number	US-201816956895-A
Country	US
Kind code	B2
Filing date	Dec 21, 2018
Priority date	Dec 22, 2017
Publication date	Nov 12, 2024
Grant date	Nov 12, 2024

Abstract

An Axial Dispersion Bioreactor (ADBR) is designed for the photoautotrophic, mixotrophic or heterotrophic growth and production of microalgae and other microorganisms (bacteria, fungi, etc.) as well as cell cultures of plants, animals, insects and others. The ADBR is equipped with a plate having a plurality of holes that moves longitudinally or axially within the bioreactor to effect superior hydrodynamic and mixing patterns. The ADBR has the advantages of providing a low-shear culture environment, superior liquid mixing, and efficient gas mass transfer.

First claim

What is claimed is:

1. A bioreactor system comprising:
a. a bioreactor vessel ( 100 ) for containing a fluid, comprising one or more sidewalls ( 120 );
b. at least one mixing plate ( 130 ) comprising a surface having a plurality of holes ( 140 ) disposed through the surface, wherein the at least one mixing plate ( 130 ) extends up to and is constrained by the one or more sidewalls ( 120 ) of the bioreactor vessel ( 100 );
c. at least one vertical guide rod ( 150 ) configured to fit through one of the plurality of holes ( 140 ) in the at least one mixing plate ( 130 ); and
d. a mechanism ( 160 ) for moving the at least one mixing plate ( 130 ) within the bioreactor vessel,
wherein the movement of the at least one mixing plate ( 130 ) enables mixing of the fluid in the bioreactor vessel to generate a hydrodynamic flow pattern.

2. The system of claim 1 , wherein the bioreactor vessel is an axial dispersion bioreactor tank.

3. The system of claim 1 , wherein the surface of the at least one mixing plate ( 130 ) comprises a flat or curved surface.

4. A bioreactor system for the cultivation of biological cultures, the system comprising:
a. a bioreactor vessel ( 100 ) for containing a liquid growth medium, comprising a bottom ( 110 ) and one or more sidewalls ( 120 );
b. one or more mixing plates ( 130 ), each comprising a plurality of through holes ( 140 ), wherein the one or more mixing plates ( 130 ) extend up to and are constrained by the one or more sidewalls ( 120 ) of the bioreactor vessel ( 100 );
c. one or more vertical guide rods ( 150 ) each configured to fit through one of the plurality of holes ( 140 ) in the one or more mixing plates ( 130 ); and
d. a mechanism ( 160 ) for moving the one or more mixing plates longitudinally or axially within the bioreactor vessel;
wherein the movement of the one or more mixing plates enables mixing of the liquid growth medium and generation of a hydrodynamic flow pattern.

5. The system of claim 4 , wherein the one or more mixing plates each comprise a shape, wherein the shape is a circle, oval, ellipse, square, rectangle, parallelogram, quadrilateral, triangle, pentagon, hexagon, heptagon, octagon, polygon, or irregular shape.

6. The system of claim 4 , wherein the one or more mixing plates each comprise a surface which is flat, rippled, textured, curved, concave, convex, zig-zag patterned, or has compound curves.

7. The system of claim 4 , wherein the plurality of through holes each comprise a shape and a size, and wherein the shapes and sizes of the holes are either uniform or varied, wherein the shapes and sizes of the holes are configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

8. The system of claim 4 , wherein the bioreactor comprises a plurality of rigid or flexible ribbons having two ends, wherein the ribbons are attached to the one or more mixing plates at one or both ends, wherein the rigid or flexible ribbons are configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

9. The system of claim 4 , wherein the movement of the one or more mixing plates is continuous or intermittent back-and-forth axial displacement at a regulated variable rate.

10. The system of claim 4 , wherein one or more gasses are sparged or bubbled through the liquid growth medium from the bottom of the bioreactor with a specified bubble size and a gas flow rate for each gas.

11. The system of claim 4 , wherein the mechanism for the movement of the one or more mixing plates comprises one of the following mechanisms:
i. a piston rod and crankshaft which connect the one or more mixing plates with an external motor, and wherein the piston rod moves the one or more mixing plates longitudinally or axially within the vessel;
ii. the one or more guide rods comprise one or more threaded rods each threaded through one of the plurality of holes in the one or more mixing plates and are attached to one or more external motors;
iii. one or more pulleys which are turned by one or more external motors, wherein one or more belts are fixed to the one or more mixing plates, pass through the one or more pulleys, and move the one or more mixing plates longitudinally or axially within the vessel; or
iv. two or more internal magnets attached to the one or more mixing plates and two or more movable exterior magnets on the other face of the one or more sidewalls, wherein movement of the exterior magnets moves the one or more mixing plates longitudinally or axially within the vessel.

12. A method of promoting the growth and production of a biological culture contained in a bioreactor, the method comprising:
a. providing a bioreactor, the bioreactor comprising:
i. a bioreactor vessel ( 100 ), comprising a bottom ( 110 ) and one or more sidewalls ( 120 );
ii. one or more mixing plates ( 130 ), each comprising a plurality of through holes ( 140 ), wherein the one or more mixing plates ( 130 ) extend up to and are constrained by the one or more sidewalls ( 120 ) of the bioreactor vessel ( 100 );
iii. one or more vertical guide rods ( 150 ) each configured to fit through one of the plurality of holes ( 140 ) in the at least one mixing plate ( 130 ); and
iv. a mechanism ( 160 ) for moving the one or more mixing plates longitudinally or axially within the bioreactor vessel;

b. providing a biological culture, wherein the biological culture is dispersed within a liquid growth medium;
c. adding the biological culture and liquid growth medium into the bioreactor; and
d. moving the one or more mixing plates longitudinally or axially within the bioreactor vessel via the mechanism;
wherein the movement of the one or more mixing plates mixes the liquid growth medium and generates a hydrodynamic flow pattern.

13. The method of claim 12 , wherein the one or more mixing plates each comprise a shape, wherein the shape is a circle, oval, ellipse, square, rectangle, parallelogram, quadrilateral, triangle, pentagon, hexagon, heptagon, octagon, polygon, or irregular shape.

14. The method of claim 12 , wherein the one or more mixing plates each comprise a surface which is flat, rippled, textured, curved, concave, convex, zig-zag patterned, or has compound curves.

15. The method of claim 12 , wherein the plurality of through holes each comprise a shape and a size, and wherein the shapes and sizes of the holes are either uniform or varied, wherein the shapes and sizes of the holes are configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

16. The method of claim 12 , wherein the bioreactor comprises a plurality of rigid or flexible ribbons having two ends, wherein the ribbons are attached to the one or more mixing plates at one or both ends, wherein the rigid or flexible ribbons are configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

17. The method of claim 12 , wherein the one or more mixing plates have a spacing which is configured to modify or enhance the hydrodynamic flow pattern or mixing of the liquid growth medium.

18. The method of claim 12 , wherein the movement of the one or more mixing plates is continuous or intermittent back-and-forth axial displacement at a regulated variable rate.

19. The method of claim 12 , wherein one or more gasses are sparged or bubbled through the liquid growth medium from the bottom of the bioreactor with a specified bubble size and a gas flow rate for each gas.

20. The method of claim 12 , wherein the

Assignees

• Univ Arizona

Inventors

• Cuello Joel L
• Mehdipour Yaser
• Mayol Andres P
• He Shiwei
• Shih Chen-Han
• Vitug Lawrence Victor

Classifications

• C12M29/06

  Nozzles; Sprayers; Spargers; Diffusers (per se B01F23/231, B01J19/26) · CPC title

• C12M27/22

  Perforated plates, discs or walls · CPC title

• B01J19/0066 Primary

  Stirrers (mixing per se B01F) · CPC title

• B01F31/441

  performing a rectilinear reciprocating movement · CPC title

• C12M27/02 Primary

  Stirrer or mobile mixing elements · CPC title