Caproate-producing bacterium with multiple substrate utilization capabilities and its applications

Patent metadata

Field	Value
Publication number	US-12129504-B2
Application number	US-202318373435-A
Country	US
Kind code	B2
Filing date	Sep 27, 2023
Priority date	Apr 6, 2021
Publication date	Oct 29, 2024
Grant date	Oct 29, 2024

Abstract

The disclosure discloses Oscillospiraceae sp. LBM10036 capable of utilizing multiple substrates and application thereof, and belongs to the fields of microbial technology and Baijiu brewing. The microorganism Oscillospiraceae sp. LBM10036 of the disclosure was preserved in the Guangdong Microbial Culture Collection Center on Sep. 21, 2020, with the preservation number GDMCC No. 61202. The Oscillospiraceae sp. LBM10036 of the disclosure has the capability to utilize diverse substrates and high product specificity, adapts to low pH, and can efficiently synthesize caproic acid under a pH of 5.0-5.5. The strain used in the disclosure can be used for strengthening microorganisms in pit mud, and improving the quality of degraded pit mud, to improve the quality of Strong aroma-type Baijiu, and also can be used for producing caproic acid from a variety of sugars and lactic acid by microbial fermentation.

First claim

What is claimed is:

1. A method for producing caproic acid, wherein the caproic acid is produced by fermentation of the Oscillospiraceae sp. LBM10036, and the Oscillospiraceae sp. LBM10036 was preserved in the Guangdong Microbial Culture Collection Center on Sep. 21, 2020, with the preservation number GDMCC No. 61202.

2. The method according to claim 1 , wherein the Oscillospiraceae sp. LBM10036 utilizes one or more of glucose, fructose, maltose, starch, and lactic acid as carbon sources.

3. The method according to claim 1 , wherein the fermentation further comprises utilizing acetate or butyrate as a cosubstrate.

4. The method according to claim 3 , wherein the fermentation is conducted at a temperature of 35-37° C. for 3-4 days, at an initial pH of fermentation of 5.5-6.5.

5. The method according to claim 2 , wherein one of the carbon sources is 10-40 g/L glucose.

6. The method according to claim 2 , wherein one of the carbon sources is 10-40 g/L fructose.

7. The method according to claim 2 , wherein one of the carbon sources is 10-40 g/L maltose.

8. The method according to claim 2 , wherein one of the carbon sources is 10-40 g/L starch.

9. The method according to claim 2 , wherein one of the carbon sources is 10-40 g/L lactic acid.

Assignees

• Univ Jiangnan

Inventors

• Xu Yan
• Ren Cong
• Wang Huilin

Classifications

• C12G3/06

  with flavouring ingredients · CPC title

• C12N1/205 Primary

  Bacterial isolates · CPC title

• C12P7/46

  Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12R2001/01

  Bacteria or Actinomycetales {; using bacteria or Actinomycetales} · CPC title