Yarn for cell culture scaffold, ply yarn comprising same and fabric comprising the same

Patent metadata

Field	Value
Publication number	US-12129489-B2
Application number	US-201716304015-A
Country	US
Kind code	B2
Filing date	May 24, 2017
Priority date	May 25, 2016
Publication date	Oct 29, 2024
Grant date	Oct 29, 2024

Abstract

A yarn including a plurality of twists formed by twisting single fiber strand or multiple fiber strands; and fiber grooves, which are spaces formed between the twists, to provide three-dimensional growth spaces and migration paths for cells. Accordingly, a cell proliferation rate and cell viability may be enhanced by creating microenvironments suitable for migration, proliferation and differentiation of cultured cells. In addition, cell clusters having more uniform shapes may be easily implemented by forming the proliferation spaces and migration paths for the cultured cells as similar as possible to each other in each scaffold. Further, the cells cultured thereby can be cultured in a suitable shape and structure to be applied to an in vitro experimental model or transplanted into the body of an animal, and can be widely applied in various products used in a cell culture or tissue engineering field.

First claim

The invention claimed is:

1. A ply yarn for a cell culture scaffold, comprising:
a slitting yarn with a plurality of twists and fiber grooves, wherein the slitting yarn has a fineness of 0.01 to 30 denier and is a nanofiber web having a basis weight of 0.1 to 20 g/m 2 in a three-dimensional network structure cut into multiple strands to have a width of 0.1 to 30 mm; and
the fiber grooves which are spaces formed between the twists and configured to provide three-dimensional growth spaces and migration paths for cell,
wherein the ply yarn comprises a plurality of macrotwists at a twist number of 100 to 5,000 twists/meter (T/m) and a twist angle of 20° to 60°, and macrofiber grooves between the plurality of macrotwists, wherein the ply yarn has a fineness of 0.5 to 1,000 denier and the macrofiber grooves are configured to provide three-dimensional growth spaces and migration paths for cells,
wherein the fiber grooves are formed continuously in the longitudinal direction of the ply yarn for a cell culture scaffold, and
wherein the ply yarn for a cell culture scaffold comprises microfiber grooves, which are spaces between the slitting yarn strands, formed on an outer surface of the ply yarn.

2. The ply yarn according to claim 1 , wherein the slitting yarn includes, as a fiber-forming component, any one or more non-biodegradable components selected from the group consisting of polystyrene (PS), polyethylene terephthalate (PET), polyethersulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), polydimethylsiloxane (PDMS), a polyamide, a polyalkylene, a poly (alkylene oxide), a poly (amino acid), a poly (allylamine), polyphosphazene and a polyethyleneoxide-polypropyleneoxide block copolymer, or any one or more biodegradable components selected from the group consisting of polycaprolactone, polydioxanone, polyglycolic acid, poly (L-lactide) (PLLA), poly (DL-lactide-co-glycolide) (PLGA), polylactic acid and polyvinyl alcohol.

3. The ply yarn according to claim 1 , wherein the slitting yarn further includes a physiologically active component which induces any one or more of adhesion, migration, growth, proliferation and differentiation of cells on an outer surface of the slitting yarn.

4. The ply yarn according to claim 3 , wherein the physiologically active component includes any one or more among any one or more compounds selected from the group consisting of a monoamine, an amino acid, a peptide, a saccharide, a lipid, a protein, a glucoprotein, a glucolipid, a proteoglycan, a mucopolysaccharide and a nucleic acid, and a cell.

5. A fabric for a cell culture scaffold, comprising the ply yarn according to claim 1 .

6. A method of forming a cell culture scaffold, comprising culturing one or more types of stem cells selected from the group consisting of totipotent stem cells, pluripotent stem cells, multipotent stem cells, oligopotent stem cells and single stem cells, and one or more types of differentiated cells selected from the group consisting of hematopoietic stem cells, liver cells, fiber cells, epithelial cells, mesothelial cells, endothelial cells, muscle cells, nerve cells, immune cells, adipose cells, cartilage cells, bone cells, blood cells and skin cells in the ply yarn of claim 1 .

Assignees

• Amogreentech Co Ltd

Inventors

• Seo In Yong
• Jang Seon Ho
• Koo Song Hee
• Kim Chan
• Lee Seoung Hoon

Classifications

• D10B2509/00

  Medical; Hygiene · CPC title

• D10B2331/04

  polyesters, e.g. polyethylene terephthalate [PET] · CPC title

• D10B2331/02

  polyamides (D10B2331/08 takes precedence) · CPC title

• D10B2321/042

  polymers of fluorinated hydrocarbons, e.g. polytetrafluoroethene [PTFE] · CPC title

• D10B2201/02

  Cotton · CPC title