Modified hematopoietic stem/progenitor and non-t effector cells, and uses thereof
US-2024041933-A1 · Feb 8, 2024 · US
Method to obtain lymphoid progenitors
US12129487B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12129487-B2 |
| Application number | US-201816498803-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 26, 2018 |
| Priority date | Mar 27, 2017 |
| Publication date | Oct 29, 2024 |
| Grant date | Oct 29, 2024 |
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Abstract
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The present invention relates to a method for preparing lymphoid progenitors. The inventors took advantage of their original and relevant Warts, Hypogammaglobulinemia, Infections and Myelokathexis (WHIM) Syndrome (WS) model and the access to blood samples from five WS patients to investigate the impact of CXCR4 desensitization on BM and extra-medullary (i.e. splenic) hematopoiesis and hematopoietic stem and progenitor cells (HSPCs) recirculation. They developed, for the first time, an original in vitro system permitting to selectively expand HSPCs to obtain lymphoid progenitors by using an original cocktail of cytokines. In particular, the present invention relates to an in vitro method for preparing lymphoid progenitors by culturing HSPCs in an appropriate culture medium comprising an effective amount of a cocktail of cytokines consisting in SCF, IL-3, IL-6, IL-7, Flt-3, and CXCL12.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of differentiating hematopoietic stem and progenitor cells (HSPCs) into lymphoid progenitor cells, said method comprising culturing the HSPCs in vitro in a culture medium comprising a cocktail of cytokines consisting of SCF, IL-3, IL-6, IL-7 and Flt-3, wherein the cytokines are present in an amount sufficient to cause differentiation of the HSPCs into lymphoid progenitor cells. 2. A method of differentiating hematopoietic stem and progenitor cells (HSPCs) into lymphoid progenitor cells, said method comprising culturing the HSPCs in vitro in a culture medium comprising a cocktail of cytokines consisting of SCF, IL-3, IL-6, IL-7 and Flt-3, and chemokine CXCL12, wherein the cytokines and the chemokine CXCL12 are present in an amount sufficient to cause differentiation of the HSPCs into lymphoid progenitor cells.
Assignees
Inventors
Classifications
- C12N5/0635Primary
B lymphocytes · CPC title
T lymphocytes · CPC title
Flt-3 ligand (CD135L, flk-2 ligand) · CPC title
Interleukin-7 (IL-7) · CPC title
Interleukin-6 (IL-6) · CPC title
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- What does patent US12129487B2 cover?
- The present invention relates to a method for preparing lymphoid progenitors. The inventors took advantage of their original and relevant Warts, Hypogammaglobulinemia, Infections and Myelokathexis (WHIM) Syndrome (WS) model and the access to blood samples from five WS patients to investigate the impact of CXCR4 desensitization on BM and extra-medullary (i.e. splenic) hematopoiesis and hematopoi…
- Who is the assignee on this patent?
- Inserm Institute National De La Sante Et De La Rech Medicale, Univ Paris Sud, Inst Nat Sante Rech Med
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0635. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 29 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 10 related publications on this page (citations in our corpus or others sharing the same primary CPC).