Systems and methods for conducting reactions and screening for reaction products

Patent metadata

Field	Value
Publication number	US-12125693-B2
Application number	US-202318378247-A
Country	US
Kind code	B2
Filing date	Oct 10, 2023
Priority date	Mar 22, 2017
Publication date	Oct 22, 2024
Grant date	Oct 22, 2024

Abstract

The invention generally relates to systems and methods for conducting reactions and screening for reaction products.

First claim

What is claimed is:

1. A method for conducting reactions and screening for reaction products, the method comprising:
providing a substrate comprising a plurality of discrete spots;
directing a liquid droplet spray discharge from a sampling probe onto a first discrete spot of the plurality of discrete spots on the substrate, wherein the first discrete spot comprises reagents for a reaction, wherein the liquid droplet spray discharge also comprises one or more reagents for the reaction and the liquid droplet spray discharge desorbs the reagents from the first discrete spot of the plurality of discrete spots on the substrate;
conducting a reaction among the reagents on the substrate and the reagents from the liquid droplet spray discharge in the liquid droplet spray discharge as the liquid droplets evaporate, thereby generating at least one ionized reaction product;
analyzing the ionized reaction product; and
repeating the method at least a second discrete spot of the plurality of discrete spots on the substrate.

2. The method according to claim 1 , wherein the sampling probe is a desorption electrospray ionization probe and the liquid droplet spray discharge is a desorption electrospray ionization active discharge.

3. The method according to claim 1 , wherein analyzing comprises:
receiving the ionized reaction product to a mass spectrometer; and
conducting a mass spectral analysis of the ionized reaction product in the mass spectrometer.

4. The method according to claim 3 , wherein the mass spectrometer is a bench-top mass spectrometer or a miniature mass spectrometer.

5. The method according to claim 1 , wherein a rate of the reaction among the reagents in the liquid droplet spray discharge is accelerated as compared to a rate of the reaction among the reagents in a bulk liquid.

6. The method according to claim 1 , wherein the substrate is a movable substrate.

7. The method according to claim 6 , wherein the method further comprises:
moving the substrate from a first discrete spot to a second discrete spot; and
repeating the method steps.

8. The method according to claim 1 , wherein the sampling probe is operably coupled to an movable arm.

9. The method according to claim 8 , wherein the method further comprises:
moving the sampling from a first discrete spot to a second discrete spot; and
repeating the method steps.

10. The method of claim 1 , wherein each of the plurality of discrete spots on the substrate comprise different reagents for a reaction.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham

Classifications

• H01J49/0013

  Miniaturised spectrometers, e.g. having smaller than usual scale, integrated conventional components · CPC title

• H01J49/145

  using chemical ionisation · CPC title

• H01J49/0463

  Desorption by laser or particle beam, followed by ionisation as a separate step (sample holder per se H01J49/0418) · CPC title

• H01J49/165 Primary

  Electrospray ionisation · CPC title

• H01J49/045

  with means for using a nebulising gas, i.e. pneumatically assisted · CPC title