Insect inhibitory proteins

Patent metadata

Field	Value
Publication number	US-12123011-B2
Application number	US-202217938016-A
Country	US
Kind code	B2
Filing date	Oct 4, 2022
Priority date	Dec 20, 2016
Publication date	Oct 22, 2024
Grant date	Oct 22, 2024

Abstract

Pesticidal proteins exhibiting toxic activity against Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran pest species are disclosed, and include, but are not limited to, TIC6280, TIC6281, TIC6282, TIC6283, TIC8808, TIC9480, TIC9257, TIC7106, TIC7017, TIC7107, TIC7108, TIC7109, TIC7110, TIC7111, TIC7589, TIC9258, and TIC9259. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the pesticidal proteins provided. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran, Hemipteran and Thysanopteran infestation are provided which contain recombinant nucleic acid sequences encoding the disclosed pesticidal proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran species pests using the disclosed pesticidal proteins are also provided.

First claim

What is claimed is:

1. A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide encoding a pesticidal protein, wherein:
a. said pesticidal protein comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs:39 and 53;
b. said pesticidal protein comprises an amino acid sequence having at least 95 % amino acid sequence identity to an amino acid sequence selected from the group consisting of: SEQ ID NOs:39 and 53; or
c. said polynucleotide comprises a nucleotide sequence selected from the group consisting of: SEQ ID NOs:38 and 52.

2. The recombinant nucleic acid molecule of claim 1 , wherein said recombinant nucleic acid molecule:
a. comprises a sequence that functions to express the pesticidal protein in a plant;
b. is expressed in a plant cell to produce a pesticidally effective amount of the pesticidal protein; or
c. is in operable linkage to a vector, and said vector is selected from the group consisting of a plasmid, phagemid, bacmid, cosmid, and a bacterial or yeast artificial chromosome.

3. The recombinant nucleic acid molecule of claim 1 , further characterized in that said recombinant nucleic acid molecule is present within a host cell, wherein said host cell is selected from the group consisting of a bacterial and a plant cell.

4. The recombinant nucleic acid molecule of claim 3 , wherein the bacterial host cell is from a genus of bacteria selected from the group consisting of Agrobacterium, Rhizobium, Bacillus, Brevibacillus, Escherichia, Pseudomonas, Klebsiella , and Erwinia;
and wherein said Bacillus species is a Bacillus cereus or a Bacillus thuringiensis , said Brevibacillus is a Brevibacillus laterosperous , and said Escherichia is an Escherichia coli.

5. The recombinant nucleic acid molecule of claim 3 , wherein said plant cell is a dicotyledonous or a monocotyledonous plant cell.

6. The recombinant nucleic acid molecule of claim 5 , wherein said plant host cell is selected from the group consisting of an alfalfa, banana, barley, bean, broccoli, cabbage, brassica, carrot, cassava, castor, cauliflower, celery, chickpea, Chinese cabbage, citrus, coconut, coffee, corn, clover, cotton, a cucurbit, cucumber, Douglas fir, eggplant, eucalyptus, flax, garlic, grape, hops, leek, lettuce, Loblolly pine, millets, melons, nut, oat, olive, onion, ornamental, palm, pasture grass, pea, peanut, pepper, pigeonpea, pine, potato, poplar, pumpkin, Radiata pine, radish, rapeseed, rice, rootstocks, rye, safflower, shrub, sorghum, Southern pine, soybean, spinach, squash, strawberry, sugar beet, sugarcane, sunflower, sweet corn, sweet gum, sweet potato, switchgrass, tea, tobacco, tomato, triticale, turf grass, watermelon, and wheat plant cell.

7. The recombinant nucleic acid molecule of claim 1 , wherein said pesticidal protein exhibits activity against an insect species of the order of Coleoptera.

8. The recombinant nucleic acid molecule of claim 7 , wherein said insect species is Western Corn Rootworm or Colorado Potato Beetle.

9. The recombinant nucleic acid molecule of claim 1 , wherein said pesticidal protein exhibits activity against an insect species of the order of Lepidoptera.

10. The recombinant nucleic acid molecule of claim 9 , wherein said insect species is Velvet bean caterpillar, Tobacco budworm, Soybean looper, or Diamondback Moth.

11. The recombinant nucleic acid molecule of claim 1 , wherein said pesticidal protein exhibits activity against an insect species of the order of Hemiptera.

12. The recombinant nucleic acid molecule of claim 11 , wherein said insect species is Southern Green Stinkbug, Neotropical Brown Stinkbug, Western Tarnished Plant Bug, and Tarnished Plant Bug.

13. A plant comprising a recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide encoding a pesticidal protein, wherein:
a. said pesticidal protein comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs:39 and 53;
b. said pesticidal protein comprises an amino acid sequence having at least 95 % or about 100% amino acid sequence identity to an amino acid sequence selected from the group consisting of: SEQ ID NOs:39 and 53;
c. said polynucleotide comprises a nucleotide sequence selected from the group consisting of: SEQ ID NOs:38 and 52; or
d. said plant exhibits a detectable amount of said pesticidal protein, wherein the pesticidal protein comprises an amino acid sequence selected from the group consisting of: SEQ ID NOs:39 and 53.

14. The plant of claim 13 , wherein said plant is a monocot plant or a dicot plant.

15. The plant of claim 13 , wherein the plant is selected from the group consisting of an alfalfa, banana, barley, bean, broccoli, cabbage, brassica, carrot, cassava, castor, cauliflower, celery, chickpea, Chinese cabbage, citrus, coconut, coffee, corn, clover, cotton, a cucurbit, cucumber, Douglas fir, eggplant, eucalyptus, flax, garlic, grape, hops, leek, lettuce, Loblolly pine, millets, melons, nut, oat, olive, onion, ornamental, palm, pasture grass, pea, peanut, pepper, pigeon pea, pine, potato, poplar, pumpkin, Radiata pine, radish, rapeseed, rice, rootstocks, rye, safflower, shrub, sorghum, Southern pine, soybean, spinach, squash, strawberry, sugar beet, sugarcane, sunflower, sweet corn, sweet gum, sweet potato, switchgrass, tea, tobacco, tomato, triticale, turf grass, watermelon, and wheat.

16. A seed from the plant of claim 13 , wherein said seed comprises said recombinant nucleic acid molecule.

17. An insect inhibitory composition comprising the recombinant nucleic acid molecule of claim 1 .

18. The insect inhibitory composition of claim 17 , further comprising a nucleotide sequence encoding at least one other pesticidal agent that is different from said pesticidal protein.

19. The insect inhibitory composition of claim 18 , wherein said at least one other pesticidal agent is selected from the group consisting of an insect inhibitory protein, an insect inhibitory dsRNA molecule, and an ancillary protein.

20. The insect inhibitory composition of claim 18 , wherein said at least one other pesticidal agent exhibits activity against one or more pest species of the orders Lepidoptera, Coleoptera, or Hemiptera.

21. The insect inhibitory composition of claim 20 , wherein said at least one other pesticidal agent is selected from the group consisting of a Cry1A, Cry1Ab, Cry1Ac, Cry1A.105, Cry1Ae, Cry1B, Cry1C, Cry1C variants, Cry1D, Cry1E, Cry1F, Cry1A/F chimeras, Cry1G, Cry1H, Cry1I, Cry1J, Cry1K, Cry1L, Cry2A, Cry2Ab, Cry2Ae, Cry3, Cry3A variants, Cry3B, Cry4B, Cry6, Cry7, Cry8, Cry9, Cry15, Cry34, Cry35, Cry43A, Cry43B, Cry51Aa1, ET29, ET33, ET34, ET35, ET66, ET70, TIC400, TIC407, TIC417, TIC431, TIC800, TIC807, TIC834, TIC853, TIC900, TIC901, TIC1201, TIC1415, TIC3131, VIP3A, VIP3B, VIP3Ab, AXMI-AXMI-, AXMI-88, AXMI-97, AXMI-102, AXMI-112, AXMI-117, AXMI-100, AXMI-115, AXMI-113, and AXMI-005, AXMI134, AXMI-150, AXMI-171, AXMI-184, AXMI-196, AXMI-204, AXMI-207, AXMI-209, AXMI-205, AXMI-218, AXMI-220, AXMI-221z, AXMI-222z, AXMI-223z, AXMI-224z and AXMI-225z, AXMI-238, AXMI-270, AXMI-279, AXMI-345, AXMI-R1 and variants thereof, IP3 and variants thereof, DIG-3, DIG-5, DIG-10 and a DIG-1.

22. A commodity product that comprises the recombinant nucleic acid molecule of claim 1 .

23. The commodity product of claim 22 , wherein the commodity product is selected from the group consisting of commodity corn bagged

Assignees

• Monsanto Technology Llc

Inventors

• Bowen David J
• Chay Catherine A
• Ciche Todd A
• Flasinski Stanislaw
• Howe Arlene R
• Milligan Jason S
• Roberts James K
• Yin Yong

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• C07K14/195

  from bacteria · CPC title

• A01N37/46

  N-acyl derivatives · CPC title

• C07K14/32 Primary

  from Bacillus (G) · CPC title