Nucleic acid constructs and methods of use
US-2016024576-A1 · Jan 28, 2016 · US
Compositions and methods for using fixed biological samples
US12117439B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12117439-B2 |
| Application number | US-202318503564-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 7, 2023 |
| Priority date | Dec 23, 2019 |
| Publication date | Oct 15, 2024 |
| Grant date | Oct 15, 2024 |
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Abstract
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The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.
First claim
Opening claim text (preview).
What is claimed is: 1. A composition comprising a compound selected from compound (8), compound (9), compound (10), or a combination thereof: 2. The composition of claim 1 , further comprising a fixed biological sample; optionally, wherein the fixed biological sample is derived from a tissue sample, a biopsy sample, or a blood sample. 3. The composition of claim 2 , wherein the composition is within a partition. 4. The composition of claim 2 , wherein the fixed biological sample comprises a sectioned tissue sample. 5. The composition of claim 1 , further comprising a protease. 6. The composition of claim 5 , wherein the protease is a thermolabile protease. 7. The composition of claim 5 , wherein the protease is a cold-active protease. 8. The composition of claim 1 , wherein the compound is compound (8). 9. The composition of claim 1 , wherein the compound is present as compound (8) formulated at a pH of about 6.5 or about 6.8. 10. A compound having the formula: 11. The compound of claim 10 , wherein the compound is formulated at a pH of about 6.5 or about 6.8. 12. A kit comprising: assay reagents; and an un-fixing agent composition comprising a compound selected from consisting of compound (8), compound (9), compound (10), and a combination thereof: 13. The kit of claim 12 , wherein the compound is compound (8). 14. The kit of claim 12 , wherein the compound is present as compound (8) formulated at a pH of about 6.5 or about 6.8. 15. The composition of claim 1 , wherein the compound is present at a concentration of about 50 mM to about 300 mM. 16. The compound of claim 10 , wherein the compound is formulated at a concentration of about 50 mM to about 300 mM. 17. The kit of claim 12 , wherein the compound is present in the composition at a concentration of about 50 mM to about 300 mM.
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Classifications
non-toxic, no Hg, no formaldehyde · CPC title
Cells of the immune system · CPC title
acting on peptide bonds (3.4) · CPC title
acting on peptide bonds (3.4) · CPC title
having three or more double bonds between ring members or between ring members and non-ring members · CPC title
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- What does patent US12117439B2 cover?
- The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cl…
- Who is the assignee on this patent?
- 10X Genomics Inc
- What technology area does this patent fall under?
- Primary CPC classification G01N33/5308. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Oct 15 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 12 related publications on this page (citations in our corpus or others sharing the same primary CPC).