Biochemical substance analysis system, method, and device

Patent metadata

Field	Value
Publication number	US-12117396-B2
Application number	US-201917763295-A
Country	US
Kind code	B2
Filing date	Sep 24, 2019
Priority date	Sep 24, 2019
Publication date	Oct 15, 2024
Grant date	Oct 15, 2024

Abstract

A biochemical substance analysis system (5) is used to detect biological characteristics of a sample in a flow cell (38), and includes a detection system (51), a scheduling system (53), a biochemical reaction system (55), and a control system (57). The scheduling system (53) is used to schedule the flow cell (38) at different sites, including sites in the detection system (51) and sites in the biochemical reaction system (55). The biochemical reaction system (55) is used to allow the sample to react in the flow cell (38). The detection system (51) is used to detect a signal from the reacted sample to obtain a signal representing the biological characteristics of the sample. The control system (57) is used to control the detection system (51), the scheduling system (53), and the biochemical reaction system (55) to cooperate. The disclosure improves automation degree and flux of the biochemical substance analysis.

First claim

What is claimed is:

1. A biochemical substance analysis system configured for detecting biological characteristics of a sample in a flow cell, comprising:
a detection system;
a scheduling system;
a biochemical reaction system; and
a control system,
wherein the scheduling system is configured to schedule the flow cell at different sites, the sites comprise sites in the detection system and sites in the biochemical reaction system; the biochemical reaction system is configured to allow the sample to perform a reaction in the flow cell; the detection system is configured to detect a signal from the reacted sample to obtain the biological characteristics of the sample; the control system is configured to control the detection system, the scheduling system, and the biochemical reaction system to cooperate with each other;
wherein the biochemical reaction system comprises a fluid system, the fluid system is configured to load a fluid to the flow cell, thereby causing the fluid to react with the sample in the flow cell;
wherein the fluid system comprises a detection reaction module, a fluid transport module, and a storage module for storing the fluid; the detection reaction module is configured to detachably mount the flow cell thereon; the fluid transport module is configured to inject the fluid stored in the storage module into the flow cell.

2. The biochemical substance analysis system of claim 1 , wherein the scheduling system comprises a transfer system, and the transfer system is configured to move the flow cell to the different sites.

3. The biochemical substance analysis system of claim 1 , wherein the biochemical reaction system further comprises a waste treatment system, and the waste treatment system is configured to collect waste discharged by the fluid system.

4. The biochemical substance analysis system of claim 3 , wherein the waste treatment system comprises a waste collection module; the waste collection module comprises a waste collection power component and a waste storage component; the waste collection power component is configured to provide power for driving the waste that lacks flowing power; the waste storage component is configured to store the collected waste.

5. The biochemical substance analysis system of claim 4 , wherein the waste treatment system further comprises a waste transport module; the waste transport module comprises a waste-discharged power component and a waste switching component; the waste switching component is a pipeline switching component configured to connect to a pipeline out of a gene sequencing system; the waste-discharged power component is configured to provide power for discharging the waste from the waste storage component.

6. The biochemical substance analysis system of claim 4 , wherein the waste collection module also comprises a waste detection component, and the waste detection component is configured to detect an amount of the waste stored in the waste storage component; when the waste detection component detects that the amount of the waste in the waste storage component exceeds a preset threshold, the control system controls the waste-discharged power component to discharge the waste.

7. The biochemical substance analysis system of claim 1 , wherein the detection system comprises a signal detection system, the signal detection system is configured to detect the signal from the reacted sample to obtain a feedback signal from the sample, and the feedback signal represents the biological characteristics of the sample; or, the detection system comprises a signal detection system and a signal processing system, the signal processing system is configured to collect the feedback signal, and the collected feedback signal is configured to generate a sequencing report.

8. The biochemical substance analysis system of claim 7 , wherein the signal detection system comprises an excitation signal transmission module, a signal channeling module, a feedback signal receiving module, and a detection fixing component; the detection fixing component is configured to detachably mount and fix the flow cell thereon; the excitation signal transmission module is configured to apply an excitation signal to the sample in the flow cell; the feedback signal receiving module is configured to receive the feedback signal from the sample excited by the excitation signal; the signal channeling module is configured to guide the excitation signal transmitted by the excitation signal transmission module to the flow cell according to a preset path, and also guide the feedback signal from the flow cell to the feedback signal receiving module according to the preset path.

9. The biochemical substance analysis system of claim 8 , wherein the signal detection system further comprises a moving component, and the moving component is configured to drive the detection fixing component to move within a certain range, to enable the signal from the sample at different positions of the flow cell to be detected.

10. The biochemical substance analysis system of claim 1 , wherein the biochemical substance analysis system is placed in a gene sequencer, and the biochemical substance analysis system is configured to receive the flow cell containing the sample through a flow cell inletting interface arranged on the gene sequencer.

11. The biochemical substance analysis system of claim 1 , wherein the fluid transport module comprises at least one fluid transport working group, the at least one fluid transport working group comprises a power component, a sample storage component, and a distribution component; the power component is configured to provide a pressure gradient for absorbing the fluid from the storage module, allowing the fluid to be temporarily stored in the sample storage component after passing through the distribution component; the power component is also configured to provide another pressure gradient for driving the fluid stored in the sample storage component to the flow cell through the distribution component.

12. The biochemical substance analysis system of claim 1 , wherein the at least one fluid transport working group comprises a plurality of fluid transport working groups; the fluid system also comprises an overall distribution component, the plurality of fluid transport working groups are connected to the overall distribution component; the overall distribution component connects different one of the plurality of fluid transport working groups to the flow cell.

13. The biochemical substance analysis system of claim 12 , wherein when one of the plurality of fluid transport working groups injects the fluid into the flow cell through the overall distribution component, another one or more of the plurality of fluid transport working groups are configured to absorb the fluid from the storage module and stores the fluid in the sample storage component.

14. The biochemical substance analysis system of claim 1 , wherein the detection reaction module comprises an inlet switch component, an outlet switch component, a bypass switch component, and an overall switch component; the inlet switch component is connected between the fluid transport module and a flow cell inlet of the flow cell; the outlet switch component is connected to the bypass switch component, the overall switch component, and a flow cell outlet of the flow cell; the overall switch component is connected to the waste treatment system.

15. The biochemical substance analysis system of claim 14 , wherein when the fluid transport module injects the fluid that will participate in a detection reaction into the detection reaction module, the fluid enters the

Assignees

• Mgi Tech Co Ltd

Inventors

• Jiang Heming
• Xing Chutian
• Yang Joon Mo
• Sui Xiangkun
• Liu Jian
• Chirita Razvan
• Wang Zhonghai
• Adams Simon Robert
• Wang Le
• Senko Mark Frederick
• Uhrich Craig Edward
• Wen Sicheng
• Kao Pin
• Mu Feng

Classifications

• C12Q1/6869

  Methods for sequencing · CPC title

• G01N35/04

  Details of the conveyor system {(G01N35/021 - G01N35/028 take precedence)} · CPC title

• G01N35/0092 Primary

  Scheduling · CPC title

• G01N21/85

  Investigating moving fluids or granular solids · CPC title

• G01N21/13 Primary

  Moving of cuvettes or solid samples to or from the investigating station {(handling materials for automatic analysis G01N35/00)} · CPC title