Plant regulatory elements and uses thereof

Patent metadata

Field	Value
Publication number	US-12116582-B2
Application number	US-202218045447-A
Country	US
Kind code	B2
Filing date	Oct 10, 2022
Priority date	Jan 10, 2019
Publication date	Oct 15, 2024
Grant date	Oct 15, 2024

Abstract

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

First claim

What is claimed is:

1. A recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a sequence with at least 96 percent sequence identity to the full length of SEQ ID NO:6 or 7 and having promoter activity;
b) a sequence comprising SEQ ID NO:6 or 7; and
c) a fragment comprising at least 175 contiguous nucleotides of SEQ ID NO:7, wherein the fragment has promoter activity;
wherein said sequence is operably linked to a heterologous transcribable DNA molecule.

2. The recombinant DNA molecule of claim 1 , wherein said sequence has at least 97 percent sequence identity to the full length of the DNA sequence of SEQ ID NO:6 or 7.

3. The recombinant DNA molecule of claim 1 , wherein said sequence has at least 99 percent sequence identity to the full length of the DNA sequence of SEQ ID NO:6 or 7.

4. The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable DNA molecule comprises a gene of agronomic interest.

5. The recombinant DNA molecule of claim 4 , wherein the gene of agronomic interest confers herbicide tolerance in plants.

6. The recombinant DNA molecule of claim 4 , wherein the gene of agronomic interest confers pest resistance in plants.

7. The recombinant DNA molecule of claim 1 , wherein the heterologous transcribable DNA molecule encodes an dsRNA, an miRNA, or a siRNA.

8. A transgenic plant cell comprising the recombinant DNA molecule of claim 1 .

9. The transgenic plant cell of claim 8 , wherein said transgenic plant cell is a monocotyledonous plant cell.

10. The transgenic plant cell of claim 8 , wherein said transgenic plant cell is a dicotyledonous plant cell.

11. A transgenic plant, or part thereof, comprising the recombinant DNA molecule of claim 1 .

12. A progeny plant of the transgenic plant of claim 11 , or a part thereof, wherein the progeny plant or part thereof comprises said recombinant DNA molecule.

13. A transgenic seed, wherein the seed comprises the recombinant DNA molecule of claim 1 .

14. A method of producing a commodity product comprising obtaining a transgenic plant or part thereof according to claim 11 and producing the commodity product therefrom.

15. The method of claim 14 , wherein the commodity product is selected from the group consisting of seeds, processed seeds, protein concentrate, protein isolate, starch, grains, plant parts, seed oil, biomass, flour and meal.

16. A method of expressing a transcribable DNA molecule comprising obtaining a transgenic plant comprising the recombinant DNA molecule of claim 1 and cultivating said plant, wherein the transcribable DNA is expressed.

17. The recombinant DNA molecule of claim 1 , wherein the DNA sequence has at least 96 percent sequence identity to the full length of SEQ ID NO:6 or 7 and has promoter activity.

18. The recombinant DNA molecule of claim 1 , wherein the DNA sequence comprises SEQ ID NO:6 or 7.

19. The recombinant DNA molecule of claim 1 , wherein the DNA sequence comprises a fragment comprising at least 175 contiguous nucleotides of SEQ ID NO:7, and wherein the fragment has promoter activity.

Assignees

• Monsanto Technology Llc
• Monsato Tech Llc

Inventors

• Flasinski Stanislaw

Classifications

• C07K14/415

  from plants · CPC title

• C12N15/8227 Primary

  Root-specific · CPC title

• C12N15/8225

  Leaf-specific, e.g. including petioles, stomata · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title