Scaffolding material for stem cell cultures and stem cell culture method using same

Patent metadata

Field	Value
Publication number	US-12116557-B2
Application number	US-201816958182-A
Country	US
Kind code	B2
Filing date	Dec 27, 2018
Priority date	Dec 27, 2017
Publication date	Oct 15, 2024
Grant date	Oct 15, 2024

Abstract

A scaffolding material for culturing a stem cell, which contains a synthetic resin, and has a nitrogen content of the synthetic resin of 0.1% by mass or more and 10% by mass or less. According to the scaffolding material for stem cell culture, the scaffolding material can have suitable hydrophilicity and strength, high fixation of stem cells after seeding, and highly efficient cell proliferation.

First claim

The invention claimed is:

1. A method for culturing a cell, comprising a step of culturing a human pluripotent stem cell on a scaffolding material in a serum-free medium containing no feeder cell or adhesive protein, wherein the scaffolding material comprises a synthetic resin, the synthetic resin comprises a polyvinyl acetal resin, and the synthetic resin has a nitrogen content of 0.1% by mass or more and 10% by mass or less.

2. The method for culturing a cell according to claim 1 , wherein the synthetic resin contains a Bronsted basic group in an amount of 1 mol % or more and 50 mol % or less.

3. The method for culturing a cell according to claim 2 , wherein the Bronsted basic group is an amine-based basic group.

4. The method for culturing a cell according to claim 2 , wherein the synthetic resin contains at least one selected from the group consisting of a structural unit having an amine structure, a structural unit having an imine structure and a structural unit having an amide structure.

5. The method for culturing a cell according to claim 1 , wherein the synthetic resin contains a structural unit having an amide structure.

6. The method for culturing a cell according to claim 1 , wherein the synthetic resin contains both of a structural unit having an imine structure and a structural unit having an amine structure.

7. The method for culturing a cell according to claim 1 , wherein the human pluripotent stem cell is a human embryonic stem cell (hESC) or a human induced pluripotent stem cell (hiPSC).

8. The method for culturing a cell according to claim 1 , wherein the polyvinyl acetal resin has a degree of acetalization of 54 mol % or more.

9. The method for culturing a cell according to claim 8 , wherein the polyvinyl acetal resin has a degree of acetalization of 60 mol % or more and 90 mol % or less.

10. The method for culturing a cell according to claim 1 , wherein the polyvinyl acetal resin is a polyvinyl butyral resin.

11. The method for culturing a cell according to claim 1 , further comprising a step of seeding a cell mass on the scaffolding material.

12. The method for culturing a cell according to claim 1 , wherein the scaffolding material is a resin film.

13. The method for culturing a cell according to claim 12 , wherein the resin film is arranged on a surface of a container.

14. The method for culturing a cell according to claim 1 , wherein the step of culturing a cell on a scaffolding material is a step of culturing a cell on a carrier containing the scaffolding material.

15. The method for culturing a cell according to claim 1 , wherein the polyvinyl acetal resin is a graft copolymer having a graft chain,
the graft chain contains at least one selected from the group consisting of a structural unit having an amine structure, a structural unit having an imine structure and a structural unit having an amide structure.

16. The method for culturing a cell according to claim 15 , wherein the graft copolymer has a unit composed of polyvinyl acetal on a main chain and a unit composed of a vinyl compound on the graft chain.

17. The method for culturing a cell according to claim 16 , wherein the vinyl compound includes at least any one of ethylene, allylamine, vinylpyrrolidone, maleic anhydride, maleimide, itaconic acid and (meth)acrylic acids.

18. The method for culturing a cell according to claim 16 , wherein the vinyl compound includes at least any one of N-vinylpyrrolidone and N-isopropylacrylamide.

Assignees

• Sekisui Chemical Co Ltd

Inventors

• Haneda Satoshi
• MANABE Yuriko
• Ishii Ryoma
• Iguchi Hiroki
• Yamauchi Hiroshi
• Omura Takahiro

Classifications

• C12N5/0696

  Artificially induced pluripotent stem cells, e.g. iPS · CPC title

• C08F124/00

  Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a heterocyclic ring containing oxygen (cyclic esters of polyfunctional acids C08F118/00; cyclic anhydrides of unsaturated acids C08F120/00, C08F122/00) · CPC title

• C12N2533/70

  Polysaccharides · CPC title

• C12N5/0606

  Pluripotent embryonic cells, e.g. embryonic stem cells [ES] (embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696) · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title