Spherical agglomeration of proteins

Patent metadata

Field	Value
Publication number	US-12110519-B2
Application number	US-201917283980-A
Country	US
Kind code	B2
Filing date	Oct 2, 2019
Priority date	Oct 25, 2018
Publication date	Oct 8, 2024
Grant date	Oct 8, 2024

Abstract

The present disclosure relates to a novel spherical agglomeration method for proteins, and protein particles made by the spherical agglomeration method. By using continuous oscillatory baffled crystallizer, the method of the present disclosure is capable of maintain the biologically activities and providing protein particles with an average particle size between 1-500 μm.

First claim

We claim:

1. A method for making spherical protein particles, wherein the method comprises:
a) dissolving a protein in water to prepare an aqueous solution of the protein;
b) providing an organic solvent mixture comprising a first organic solvent, which is immiscible with water, and a second organic solvent, which is miscible with water;
c) providing a continuous oscillatory baffled crystallizer with an inlet and an outlet, and allowing the organic solvent mixture to run from the inlet of the continuous oscillatory baffled crystallizer and out from the outlet of the continuous oscillatory baffled crystallizer;
d) injecting the aqueous solution of the protein dropwise through a point between the inlet and the outlet of the continuous oscillatory baffled crystallizer to form droplets of the aqueous protein solution, whereupon the second organic solvent diffuses into the droplets of the aqueous protein solution and the first organic solvent suspends the droplets of the aqueous protein solution; and
e) allowing a formation of protein crystals, wherein the formed protein crystals agglomerate to form substantially spherical protein particles towards the outlet of the continuous oscillatory baffled crystallizer,
wherein the protein is lysozyme, the first organic solvent is ethyl acetate, and the second organic solvent is ethanol.

2. The method of claim 1 , wherein the ethyl acetate and the ethanol are present in the ratio of about 95:5% v/v.

Assignees

• Purdue Research Foundation

Inventors

• Nagy Zoltan K
• Oliva Joseph A
• Pal Kanjakha

Classifications

• C12Y302/01017

  Lysozyme (3.2.1.17) · CPC title

• C12N9/98

  Preparation of granular or free-flowing enzyme compositions (C12N9/96 takes precedence) · CPC title

• C07K1/026

  by fragment condensation in solution · CPC title

• A61K38/47

  acting on glycosyl compounds (3.2), e.g. cellulases, lactases · CPC title

• C12N9/2462 Primary

  Lysozyme (3.2.1.17) · CPC title