Engineered purine nucleoside phosphorylase variant enzymes
US-11162105-B2 · Nov 2, 2021 · US
Engineered purine nucleoside phosphorylase variant enzymes
US12110493B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12110493-B2 |
| Application number | US-202117490215-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 30, 2021 |
| Priority date | Jul 9, 2018 |
| Publication date | Oct 8, 2024 |
| Grant date | Oct 8, 2024 |
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Abstract
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The present invention provides engineered purine nucleoside phosphorylase (PNP) enzymes, polypeptides having PNP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PNP enzymes are also provided. The present invention further provides compositions comprising the PNP enzymes and methods of using the engineered PNP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
First claim
Opening claim text (preview).
We claim: 1. A polynucleotide sequence encoding an engineered purine nucleoside phosphorylase comprising a polypeptide sequence having at least 85% sequence identity to SEQ ID NO:2, wherein the polypeptide sequence of said engineered purine nucleoside phosphorylase comprises a substitution at position 65 and wherein the polypeptide sequence is numbered with reference to SEQ ID NO: 2, and wherein said engineered purine nucleoside phosphorylase comprises improved activity on compound 3 as compared to wild-type E. coli purine nucleoside phosphorylase. 2. The polynucleotide sequence of claim 1 , wherein said polynucleotide sequence is operably linked to a control sequence. 3. The polynucleotide sequence of claim 1 , wherein said polynucleotide sequence is codon optimized. 4. The polynucleotide sequence of claim 1 , wherein said polynucleotide sequence comprises the polynucleotide sequence as set forth in SEQ ID NO: 5. 5. An expression vector comprising the polynucleotide sequence of claim 1 . 6. A host cell comprising the expression vector of claim 5 . 7. A host cell comprising the polynucleotide sequence of claim 1 . 8. A method of producing an engineered purine nucleoside phosphorylase in a host cell, comprising culturing the host cell of claim 7 , under suitable conditions, such that the engineered purine nucleoside phosphorylase is produced. 9. The method of claim 8 , further comprising recovering the engineered purine nucleoside phosphorylase from the culture and/or host cell. 10. The method of claim 9 , further comprising a step of purifying said engineered purine nucleoside phosphorylase.
Assignees
Inventors
Classifications
Purine-nucleoside phosphorylase (2.4.2.1) · CPC title
- C12N9/1077Primary
Pentosyltransferases (2.4.2) · CPC title
- C12N15/63Primary
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title
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- What does patent US12110493B2 cover?
- The present invention provides engineered purine nucleoside phosphorylase (PNP) enzymes, polypeptides having PNP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PNP enzymes are also provided. The present invention further provides compositions comprising the PNP enzymes and methods o…
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1077. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 08 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).