Meta fluorescence tissue mimicking phantom of imaging method and system

Patent metadata

Field	Value
Publication number	US-12109073-B2
Application number	US-202318398177-A
Country	US
Kind code	B2
Filing date	Dec 28, 2023
Priority date	Feb 8, 2023
Publication date	Oct 8, 2024
Grant date	Oct 8, 2024

Abstract

A meta fluorescence tissue mimicking phantom of imaging method and system. The system includes a fluorescence acquisition module configured to acquire spectral information, contrast information and light intensity information of fluorescence molecules in biological tissue, store the spectral information, the contrast information and the light intensity information in a computer, and transmit the spectral information, the contrast information and the light intensity information to a fluorescence reproduction module through a computer communication port; a fluorescence reproduction module configured to generate a fluorescence tissue mimicking phantom according to the spectral information, the contrast information and the light intensity information; and a meta fluorescence tissue-mimicking phantom imaging module configured to perform imaging on the fluorescence tissue mimicking phantom.

First claim

What is claimed is:

1. A meta fluorescence tissue mimicking phantom of imaging system, comprising:
a fluorescence acquisition module configured to acquire a spectral information, a contrast information and a light intensity information of fluorescence molecules in a biological tissue, store the spectral information, the contrast information and the light intensity information in a computer, and transmit the spectral information, the contrast information and the light intensity information to a fluorescence reproduction module by a computer communication port;
the fluorescence reproduction module configured to generate a fluorescence tissue mimicking phantom according to the spectral information, the contrast information and the light intensity information; and
a meta fluorescence tissue-mimicking phantom imaging module configured to perform imaging on the fluorescence tissue mimicking phantom;
wherein the fluorescence reproduction module comprises:
a laser configured to emit super-continuum spectrum beams, regulate an optical power of the super-continuum spectrum beams according to the light intensity information, and transmit the super-continuum spectrum beams to a spectrum regulation unit;
the spectrum regulation unit configured to regulate the super-continuum spectrum beams according to the spectral information to obtain regulated spectral beams, and transmit the regulated spectral beams to a contrast regulation unit; and
the contrast regulation unit configured to regulate a spatial contrast distribution of the regulated spectral beams according to the contrast information to obtain fluorescent beams, and project the fluorescent beams to generate the fluorescence tissue mimicking phantom;
wherein the laser comprises a super-continuum laser, and the super-continuum spectrum beams comprise lights with a continuously changing wavelength;
wherein the spectrum regulation unit comprises:
a coupling lens configured to collimate on the super-continuum spectrum beams to obtain collimated beams, and transmit the collimated beams to a first grating;
the first grating configured to generate dispersion of the collimated beams to obtain a unfocused dispersed light, and transmit the unfocused dispersed light to a first focusing lens;
the first focusing lens configured to transform the unfocused dispersed light into a focused dispersed light, and transmit the focused dispersed light to a first spatial light modulation device;
the first spatial light modulation device configured to regulate a spectral composition of the focused dispersed light according to the spectral information to allow the spectral composition of the focused dispersed light be consistent with the spectral information to obtain regulated focused dispersed light, and transmit the regulated focused dispersed light to a second focusing lens; wherein the first spatial light modulation device adopts a meta micromirror device (DMD);
the second focusing lens configured to transform the regulated focused dispersed light into regulated unfocused dispersed light, and transmit the regulated unfocused dispersed light to a second grating;
the second grating configured to combine the regulated unfocused dispersed light into a beam of light to obtain a combined beam light, and transmit the combined beam light to a light homogenizer; and
the light homogenizer configured to dodge on the combined beam light to obtain the regulated spectral beams; and

wherein the contrast regulation unit comprises:
a second spatial light modulation device configured to adopt the meta micromirror device (DMD) and regulates the spatial contrast distribution of the regulated spectral beams according to the contrast information to allow the spatial contrast distribution of the regulated spectral beams be consistent with the contrast information to obtain the fluorescent beams; and
a projection lens configured to project the fluorescent beams to generate the fluorescence tissue mimicking phantom.

2. The meta fluorescence tissue mimicking phantom of imaging system according to claim 1 , wherein the fluorescence acquisition module comprises:
a spectrometer configured to acquire a fluorescence emission spectrum information of the fluorescence molecules in the biological tissue;
an optical camera configured to acquire a fluorescence image contrast information of the fluorescence molecules in the biological tissue; and
an optical power meter configured to acquire the light intensity information of the fluorescence molecules in the biological tissue; and
wherein the fluorescence emission spectrum information is the spectral information, the fluorescence image contrast information is the contrast information, and the spectral information, the contrast information and the light intensity information are stored in the computer and transmitted to the fluorescence reproduction module by the computer communication port.

3. The meta fluorescence tissue mimicking phantom of imaging system according to claim 1 , wherein the meta fluorescence tissue-mimicking phantom imaging module comprises:
an imaging lens configured to perform imaging on the fluorescence tissue mimicking phantom to obtain an imaging result, and transmit the imaging result to a camera; and
the camera configured to transmit the imaging result to the computer, and analyze parameters of resolution, sensitivity and/or depth-of-field on the imaging result in the computer.

4. Use of the meta fluorescence tissue mimicking phantom of imaging system according to claim 1 in traceability of International System of Units for tissue mimicking phantoms, wherein an optical power meter with a clear aperture of D cm is adopted and placed at a position with a distance of R cm from a tissue mimicking phantom, an optical power of the current tissue mimicking phantom is measured to be E mW, a luminous area (A) is calculated by A=¼*pi*D 2 , a solid angle (theta) is calculated by theta-A/R 2 , and a phantom radiation quantity is calculated by E/A/theta, in mW/cm 2 /sr.

5. A meta fluorescence tissue mimicking phantom of imaging method adopting the system according to claim 1 , comprising:
step S 1 : acquiring, by a fluorescence acquisition module, a spectral information, a contrast information and a light intensity information of fluorescence molecules in a biological tissue, storing the spectral information, the contrast information and the light intensity information in a computer, and transmitting the spectral information, the contrast information and the light intensity information to a fluorescence reproduction module by a computer communication port;
step S 2 : generating, by the fluorescence reproduction module, a fluorescence tissue mimicking phantom according to the spectral information, the contrast information and the light intensity information; and
step S 3 : imaging, by a meta fluorescence tissue-mimicking phantom imaging module, on the fluorescence tissue mimicking phantom.

Assignees

• Zhejiang Lab

Inventors

• Yang Qing
• Qi Ji
• Chen Xiaopeng

Classifications

• A61B1/00057

  provided with means for testing or calibration · CPC title

• A61B1/043

  for fluorescence imaging · CPC title

• G06T7/0012

  Biomedical image inspection · CPC title

• G06T2207/10064

  Fluorescence image · CPC title

• G06T2207/30024

  Cell structures in vitro; Tissue sections in vitro · CPC title