Growth-independent inhibition of aflatoxin biosynthesis with selected nanoparticles

Patent metadata

Field	Value
Publication number	US-12108764-B2
Application number	US-201815911327-A
Country	US
Kind code	B2
Filing date	Mar 5, 2018
Priority date	Mar 3, 2017
Publication date	Oct 8, 2024
Grant date	Oct 8, 2024

Abstract

Methods for inhibiting fungal secondary metabolisms are described. Secondary metabolisms inhibited by the methods can include those responsible for expression of mycotoxins such as aflatoxin. Methods can include inhibition of aflatoxin production without inhibiting fungal growth by contacting a fungus with a suspension including silver nanoparticles that can include a surface coating. The methods can be utilized in preventing fungal-caused destruction of plant species (e.g., crop species). Disclosed methods encompass utilization of silver nanoparticles at low concentrations of from about 10 picograms per liter to about 1 microgram per liter.

First claim

What is claimed is:

1. A method for inhibiting production of an aflatoxin in a fungus comprising:
forming a first suspension of silver nanoparticles, the silver nanoparticles comprising a surface coating, the coated silver nanoparticles having an average size of from 15 nanometers to 30 nanometers as determined by transmission electron microscopy;
separating the coated silver nanoparticles from the first suspension by ultrafiltration in a diafiltration mode;
redispersing the coated silver nanoparticles in a second suspension, the second suspension including the coated silver nanoparticles at a concentration of from 25 nanograms per milliliter to 60 nanograms per milliliter;
contacting the fungus with the second suspension, wherein during a period of time in which the fungus is in contact with the suspension, fungal aflatoxin production is inhibited with no significant decrease in fungal growth rate as expressed as dry weight accumulation per unit time of the contact.

2. The method of claim 1 , wherein the fungus comprises a mycotoxin-producing filamentous fungus.

3. The method of claim 1 , wherein the fungus comprises Aspergillus parasiticus.

4. The method of claim 1 , the coated silver nanoparticles having an average size of 20 nanometers.

5. The method of claim 1 , wherein the coating comprises a citrate.

6. The method of claim 1 , wherein the coating comprises a polymer.

7. The method of claim 1 , the method decreasing generation of reactive oxygen species by the fungus during the period of time of contact.

8. The method of claim 1 , the method inhibiting transcription of one or more genes associated with aflatoxin biosynthesis during the period of time of contact.

9. The method of claim 1 , the method inhibiting the global regulators of secondary metabolism laeA and veA during the period of time of contact.

10. The method of claim 1 , the second suspension including the coated silver nanoparticles at a concentration of 50 nanograms per milliliter.

11. The method of claim 6 , wherein the coating comprises polyvinyl pyrrolidone.

12. The method of claim 1 , wherein the second suspension includes the coated silver nanoparticles at a concentration of from 40 nanograms per milliliter to 60 nanograms per milliliter.

13. The method of claim 1 , wherein the second suspension includes the coated silver nanoparticles at a concentration of 25 nanograms per milliliter.

Assignees

• Univ South Carolina

Inventors

• Chanda Anindya
• Lead Jamie

Classifications

• A01N25/28

  Microcapsules {or nanocapsules} · CPC title

• A01N59/16 Primary

  Heavy metals; Compounds thereof · CPC title