Multi-array impedimetric biosensors for the detection of concussion and traumatic brain injuries

Patent metadata

Field	Value
Publication number	US-12105087-B2
Application number	US-201716467116-A
Country	US
Kind code	B2
Filing date	Dec 11, 2017
Priority date	Dec 9, 2016
Publication date	Oct 1, 2024
Grant date	Oct 1, 2024

Abstract

The invention includes an ex-situ biosensors that impedimetrically detect one or more target biomarkers of brain injury or disease in a bodily fluid sample derived from a patient. The biosensors include a multi-array of vertically aligned platinum wires having immobilized thereon antibody and/or aptamer that specifically and selectively bind to the one or more target biomarkers. An electrochemical impedance signal is generated, and a change in electrochemical impedance is indicative of the presence of the one or more target biomarker(s) in the bodily fluid sample. The biosensors are point-of-care, on-demand devices useful in a medical and domestic environment.

First claim

The invention claimed is:

1. A portable, ex-situ system to impedimetrically detect one or more target biomarkers related to brain injury or disease in a bodily fluid sample of a patient, comprising:
a conducting material interface, comprising:
an epoxy resin; and
a multi-array of vertically aligned conducting metal wires selected from the group consisting of platinum wires, gold wires, iridium wires, ruthenium wires, palladium wires, osmium wires, and alloys and combinations thereof, each of the plurality of conducting metal wires having a first end and an opposite second end with the first end cast in the epoxy resin;

a thiol-based compound applied to the second end of the plurality of conducting wires to form a thiol treated surface;
an immobilization agent applied to the thiol treated surface; and
at least one biotinylated antibody and/or aptamer applied to the immobilization agent, selected to bind with the immobilization agent and the one or more target biomarkers related to brain injury or disease;
a signaling agent comprising an electrochemical impedance signal generated by binding of the at least one biotinylated antibody and/or aptamer with the one or more target biomarkers related to brain injury or disease; and
the bodily fluid sample derived from the patient and in contact with the at least one biotinylated antibody and/or aptamer to detect the one or more target biomarkers related to brain injury or disease,
wherein the system is structured such that in its use a change in electrochemical impedance indicates a presence of the one or more target biomarkers related to brain injury or disease in the bodily fluid sample and binding of the at least one biotinylated antibody and/or aptamer with the one or more target biomarkers in the bodily fluid sample, and
wherein the system is structured such that in its use an absence of a change in electrochemical impedance indicates an absence of the one or more target biomarkers related to brain injury or disease in the bodily fluid sample.

2. The system of claim 1 , wherein the bodily fluid sample is a blood sample.

3. The system of claim 1 , wherein the one or more target biomarkers related to brain injury or disease are selected from the group consisting of Tau proteins, Glial Fibrilar Acidic Protein (GFAP) and Ubiquitin C-Terminal Hydrolase L1 (UCH-L1).

4. The system of claim 1 , wherein the immobilization agent is selected from the group consisting of avidin, streptavidin, neutravidin and mixtures thereof.

5. The system of claim 1 , wherein the antibody and/or aptamer sensor is effective to impedimetrically detect simultaneously a plurality of target biomarkers related to brain injury or disease in the bodily fluid sample.

6. The system of claim 1 , wherein the multi-array of vertically aligned conducting material wires are arranged in a concentric configuration.

Assignees

• Univ Of Pittsburgh—Of The Commonwealth System Of Higher Education
• Univ Pittsburgh Commonwealth Sys Higher Education

Inventors

• Kumta Prashant
• Patil Mitali
• Okonkwo David
• Kormos Robert

Classifications

• G01N2800/2871

  Cerebrovascular disorders, e.g. stroke, cerebral infarct, cerebral haemorrhage, transient ischemic event · CPC title

• G01N33/5438

  Electrodes · CPC title

• G01N27/00

  Investigating or analysing materials by the use of electric, electrochemical, or magnetic means (G01N3/00 – G01N25/00 take precedence; measurement or testing of electric or magnetic variables or of electric or magnetic properties of materials G01R) · CPC title

• G01N33/6893

  related to diseases not provided for elsewhere · CPC title

• C07K16/18 Primary

  against material from animals or humans · CPC title