Enzyme with acyl transfer function and application thereof

Patent metadata

Field	Value
Publication number	US-12104180-B2
Application number	US-202017432099-A
Country	US
Kind code	B2
Filing date	Dec 7, 2020
Priority date	Feb 10, 2020
Publication date	Oct 1, 2024
Grant date	Oct 1, 2024

Abstract

An enzyme with an acyl transfer function has an amino acid sequence identical to a SEQ ID NO:1, which is capable of acylation modification for macrolactins, filipins macrolides, chloramphenicol, and glycosylated piericidin A. An application of an acyltransferase of the present invention is to bind an acyl group of an acyl donor to macrolide compounds, chloramphenicol and glycosylated piericidins. The enzyme with the acyl transfer function can improve pharmacological activity of the macrolide compounds through acylation reaction of macrolactins, thereby improving bioavailability, enhancing efficacy, and reducing toxic as well as side effects, which provides a new strategy for the drug development of macrolide compounds.

First claim

What is claimed is:

1. An acyltransferase, comprising:
a) a protease having a sequence identical to a SEQ ID NO:1;
b) a protease obtained by substituting R at position 166 or substituting E at position 128 of the protease in a), which has an acyltransferase activity of the protease in a).

2. The acyltransferase, as recited in claim 1 , wherein the protein substituting in b) is realized by substituting R at position 166 with A, G, K or Q.

3. The acyltransferase, as recited in claim 1 , wherein the protein substituting in b) is realized by substituting E at position 128 with A or Q.

Assignees

• Ocean Univ China

Inventors

• Li Wenli
• Xiao Fei
• Liu Yang
• Li Huayue

Classifications

• C12P17/08

  containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons · CPC title

• C12P19/58

  having an oxygen atom of the saccharide radical directly bound through only acyclic carbon atoms to a non-saccharide heterocyclic ring, e.g. bleomycin, phleomycin · CPC title

• C12P13/02

  Amides, e.g. chloramphenicol {or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes (peptides C12P21/00 or C07K)} · CPC title

• C12N15/70

  Vectors or expression systems specially adapted for E. coli · CPC title

• C12N9/1025 Primary

  Acyltransferases (2.3) · CPC title