Insect inhibitory proteins

Patent metadata

Field	Value
Publication number	US-12104162-B2
Application number	US-202217854198-A
Country	US
Kind code	B2
Filing date	Jun 30, 2022
Priority date	Jul 8, 2021
Publication date	Oct 1, 2024
Grant date	Oct 1, 2024

Abstract

Pesticidal protein exhibiting toxic activity against Lepidopteran and Hemipteran pest species are disclosed, and include, but are not limited to, TIC2199. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the disclosed pesticidal protein. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Lepidopteran and Hemipteran species pests using TIC2199 pesticidal protein are also provided.

First claim

What is claimed is:

1. A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding a pesticidal protein or pesticidal fragment thereof, wherein:
a. said pesticidal protein comprises the amino acid sequence of SEQ ID NOs:2, 4, 7, 10, 13, 15, or 17; or
b. said pesticidal protein comprises an amino acid sequence having at least 99% amino acid sequence identity to SEQ ID NOs:2, 4, 7, 10, 13, 15, or 17;
wherein said pesticidal protein exhibits activity against a Lepidopteran or a Hemipteran insect.

2. The recombinant nucleic acid molecule of claim 1 , wherein: said recombinant nucleic acid molecule is in operable linkage with a vector, and said vector is selected from the group consisting of a plasmid, phagemid, bacmid, cosmid, bacterial artificial chromosome, and a yeast artificial chromosome.

3. A host cell comprising the recombinant nucleic acid molecule of claim 1 , wherein said host cell is selected from the group consisting of a bacterial cell and a plant cell.

4. The host cell of claim 3 , wherein said host cell is a bacterial host cell selected from the group consisting of: Agrobacterium, Rhizobium, Bacillus, Brevibacillus, Escherichia, Pseudomonas, Klebsiella, Pantoea , and Erwinia.

5. The bacterial host cell of claim 4 , wherein said Bacillus is Bacillus cereus or Bacillus thuringiensis , said Brevibacillus is a Brevibacillus laterosperous , and said Escherichia is an Escherichia coli.

6. The host cell of claim 3 , wherein said plant cell is selected from the group consisting of alfalfa, banana, barley, bean, broccoli, cabbage, brassica, canola, carrot, cassava, castor, cauliflower, celery, chickpea, Chinese cabbage, citrus, coconut, coffee, corn, clover, cotton, cucurbit, cucumber, Douglas fir, eggplant, eucalyptus, flax, garlic, grape, hops, leek, lettuce, Loblolly pine, millet, melon, nut, oat, olive, onion, ornamental plant, palm, pasture grass, pea, peanut, pepper, pigeonpea, pine, potato, poplar, pumpkin, Radiata pine, radish, rapeseed, rice, rootstock, rye, safflower, shrub, sorghum, Southern pine, soybean, spinach, squash, strawberry, sugar beet, sugarcane, sunflower, sweet gum, sweet potato, switchgrass, tea, tobacco, tomato, triticale, turf grass, watermelon, and wheat plant cell.

7. The recombinant nucleic acid molecule of claim 1 , wherein said Lepidopteran insect is selected from the group consisting of: Black cutworm, Corn earworm, Cabbage looper worm, European corn borer, Fall armyworm, Southern armyworm, Soybean looper, Southwestern corn borer, Sugarcane borer, Velvet bean caterpillar, Western bean cutworm, and Lesser cornstalk borer.

8. The recombinant nucleic acid molecule of claim 1 , wherein said Hemipteran insect is selected from the group consisting of: Tarnished plant bug and Neotropical brown stink bug.

9. A plant or part thereof comprising the recombinant nucleic acid molecule of claim 1 .

10. The plant of claim 9 , wherein the plant is selected from the group consisting of alfalfa, banana, barley, bean, broccoli, cabbage, brassica, canola, carrot, cassava, castor, cauliflower, celery, chickpea, Chinese cabbage, citrus, coconut, coffee, corn, clover, cotton, cucurbit, cucumber, Douglas fir, eggplant, eucalyptus, flax, garlic, grape, hops, leek, lettuce, Loblolly pine, millet, melon, nut, oat, olive, onion, ornamental plant, palm, pasture grass, pea, peanut, pepper, pigeonpea, pine, potato, poplar, pumpkin, Radiata pine, radish, rapeseed, rice, rootstock, rye, safflower, shrub, sorghum, Southern pine, soybean, spinach, squash, strawberry, sugar beet, sugarcane, sunflower, sweet gum, sweet potato, switchgrass, tea, tobacco, tomato, triticale, turf grass, watermelon, and wheat.

11. The plant of claim 9 , wherein the part of the plant thereof is a seed, and wherein said seed comprises said recombinant nucleic acid molecule.

12. An insect inhibitory composition comprising the recombinant nucleic acid molecule of claim 1 and the pesticidal protein or pesticidal fragment thereof encoded by the nucleic acid molecule.

13. The insect inhibitory composition of claim 12 , further comprising a nucleotide sequence encoding at least one other pesticidal agent that is different from said pesticidal protein.

14. The insect inhibitory composition of claim 13 , wherein said at least one other pesticidal agent:
a. is selected from the group consisting of an insect inhibitory protein, an insect inhibitory dsRNA molecule, a chemical molecule and an ancillary protein, wherein said at least one other pesticidal agent is toxic to the same pest as the pesticidal protein or pesticidal fragment thereof;
b. exhibits activity against one or more pest species of the orders Lepidoptera, Coleoptera, or Hemiptera; or
c. is selected from the group consisting of Cry1A, Cry1Ab, Cry1Ac, Cry1A.105, Cry1Ae, Cry1B, Cry1C, Cry1C variants, Cry1D, Cry1D variants, Cry1E, Cry1F, Cry1A/F chimeras, Cry1G, Cry1H, Cry1I, Cry1J, Cry1K, Cry1L, Cry2A, Cry2Ab, Cry2Ae, Cry3, Cry3A variants, Cry3B, Cry4B, Cry6, Cry7, Cry8, Cry9, Cry15, Cry34, Cry35, Cry43A, Cry43B, Cry51Aa1, ET29, ET33, ET34, ET35, ET66, ET70, TIC400, TIC407, TIC417, TIC431, TIC800, TIC807, TIC834, TIC853, TIC900, TIC901, TIC1201, TIC1415, TIC2160, TIC3131, TIC836, TIC860, TIC867, TIC869, TIC1100, VIP3A, VIP3B, VIP3Ab, AXMI-88, AXMI-97, AXMI-102, AXMI-112, AXMI-117, AXMI-100, AXMI-115, AXMI-113, AXMI-005, AXMI134, AXMI-150, AXMI-171, AXMI-184, AXMI-196, AXMI-204, AXMI-207, AXMI-209, AXMI-205, AXMI-218, AXMI-220, AXMI-221z, AXMI-222z, AXMI-223z, AXMI-224z, AXMI-225z, AXMI-238, AXMI-270, AXMI-279, AXMI-345, AXMI-335, AXMI-R1, AXMI-R1 variants, IP3, IP3 variants, DIG-3, DIG-5, DIG-10, DIG-657, DIG-11 protein, IDP102Aa, IDP102Aa homologs, IDP110Aa, IDP110Aa homologs, TIC868, Cry1Dal_7, BCW003, TIC1100, TIC867, TIC867_23, TIC6757, TIC7641, IDP072Aa, TIC5290, TIC3668, TIC3669, TIC3670, IDP103, IDP103 homologs, PIP-50, PIP-65, PIP-65 homologs, PIP-83, PIP-83 homologs, and Cry1B.34.

15. The insect inhibitory composition of claim 12 , defined as comprising a plant cell that expresses the pesticidal protein from the recombinant nucleic acid molecule.

16. A commodity product produced from the plant, or part thereof, of claim 9 , wherein the commodity product comprises a detectable amount of said recombinant nucleic acid molecule and said pesticidal protein or pesticidal fragment thereof.

17. The commodity product of claim 16 , selected from the group consisting of commodity corn bagged by a grain handler, corn flakes, corn cakes, corn flour, corn meal, corn syrup, corn oil, corn silage, corn starch, corn cereal, soybean, rice, wheat, sorghum, pigeon pea, peanut, fruit, melon, juices, concentrates, jams, jellies, marmalades, whole cotton seed, processed cotton seed, cotton oil, lint, fiber, paper, biomasses, whole soybean seed, processed soybean seed, soybean oil, soybean protein, soybean meal, soybean flour, soybean flakes, soybean bran, soybean milk, soybean cheese, soybean wine, animal feed comprising soybean, paper comprising soybean, cream comprising soybean, soybean biomass, and fuel products.

18. A method of producing progeny seed comprising the recombinant nucleic acid molecule of claim 1 , the method comprising:
a. planting a first seed comprising the recombinant nucleic acid molecule;
b. growing a plant from the seed of step a; and
c. harvesting the progeny seed from the plants, wherein said harvested seed comprises said recombinant nucleic acid molecule.

19. A plant resistant to insect infestation, wherein the cells of sa

Assignees

• Monsanto Technology Llc

Inventors

• Bowen David J
• Chay Catherine A
• Howe Arlene R
• Wegener Kimberley M

Classifications

• A01N63/23

  B. thuringiensis · CPC title

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title

• A01P7/04

  Insecticides · CPC title

• C07K14/32 Primary

  from Bacillus (G) · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title