Acoustic perfusion devices
US-2017137774-A1 · May 18, 2017 · US
Fluid system for producing extracellular vesicles and related method
US12098355B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12098355-B2 |
| Application number | US-201816627013-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 29, 2018 |
| Priority date | Jun 30, 2017 |
| Publication date | Sep 24, 2024 |
| Grant date | Sep 24, 2024 |
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- Title
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Abstract
Official abstract text for this publication.
A fluidic system for producing extracellular vesicles from producer cells, including at least one container, a liquid medium contained by the container and producer cells, characterised in that it also includes microcarriers suspended in the liquid medium, the majority of producer cells being adherent to the surface of the microcarriers, and a liquid medium agitator, the agitator and the dimensions of the container being adapted to control a turbulent flow of the liquid medium in the container.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for ex vivo production of extracellular vesicles from producer cells, comprising: controlling an agitator causing a turbulent flow of a liquid medium in a container, the liquid medium being agitated for more than thirty minutes, the container comprising an output, the liquid medium comprising producer cells adhering on the surface of the microcarriers, the microcarriers being suspended in the liquid medium; and collecting the liquid medium comprising extracellular vesicles at the output of the container, wherein the agitator is controlled to cause the turbulent flow of the liquid medium to form vortices so that the turbulent flow has a Kolmogorov length of more than or equal to 33 μm and less than or equal to 75 μm, and wherein said turbulent flow method allows the production of the extracellular vesicles with a higher yield as compared to a serum starvation method for producing extracellular vesicles. 2. The method according to claim 1 , wherein a separator depletes part of the liquid medium collected at the output of the container of extracellular vesicle, and wherein the part of the liquid medium is reintroduced into the container.
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Classifications
Filters; Permeable or porous membranes or plates, e.g. dialysis · CPC title
- C12M25/16Primary
Particles; Beads; Granular material; Encapsulation (chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00) · CPC title
by mechanical forces; Stirring; Trituration; Comminuting (crushing, pulverizing, disintegrating in general B02C) · CPC title
Stirrer or mobile mixing elements · CPC title
- C12M47/10Primary
Separation or concentration of fermentation products (bioreactors combined with means for distillation or extraction of liquid fuel C12M43/02) · CPC title
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- What does patent US12098355B2 cover?
- A fluidic system for producing extracellular vesicles from producer cells, including at least one container, a liquid medium contained by the container and producer cells, characterised in that it also includes microcarriers suspended in the liquid medium, the majority of producer cells being adherent to the surface of the microcarriers, and a liquid medium agitator, the agitator and the dimens…
- Who is the assignee on this patent?
- Univ Paris, Centre Nat Rech Scient, Genethon
- What technology area does this patent fall under?
- Primary CPC classification C12M25/16. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 24 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).