Analytical cartridge for soil testing and related methods

Patent metadata

Field	Value
Publication number	US-12090475-B2
Application number	US-201917052419-A
Country	US
Kind code	B2
Filing date	Apr 11, 2019
Priority date	May 1, 2018
Publication date	Sep 17, 2024
Grant date	Sep 17, 2024

Abstract

A rotary analysis apparatus and related methods are disclosed. The apparatus generally includes a rotary machine operable to rotate or spin a removable disk-type analytical cartridge. The cartridge includes a plurality of fluidly isolated processing trains for processing multiple samples simultaneously. Each process train includes an extractant mixing chamber, slurry filtration chamber, supernatant collection chamber, and reagent mixing chamber in fluid communication. In one use, soil sample slurry is prepared and added to the extractant mixing chamber. The slurry is mixed with an extractant by rotating the cartridge to separate out an analyte from the mixture. A sediment filter in the filtration chamber deliquifies and traps soil particles to produce clear supernatant. A color changing reagent or fluorescent agent may be mixed with the collected supernatant for subsequent colorimetric, fluorescent, turbidimetric, or other type of analysis.

First claim

What is claimed is:

1. An analytical cartridge for fluid testing, the cartridge comprising:
a centerline axis;
a main body defining a plurality of sample processing trains arranged around the centerline axis, the main body having a mounting opening configured for mounting to a spindle of a rotary machine;
each processing train including an extractant mixing chamber having a slurry fill hole for introducing a slurry and an extractant, and a reagent mixing chamber fluidly coupled to the extractant mixing chamber;
each processing train including a sediment filter configured to deliquify the slurry for producing a supernatant collected in the reagent mixing chamber for analysis;
an annular filter ring separately attached to a bottom of the main body of the cartridge;
wherein the annular filter ring further comprises a plurality of upwardly elongated raised filter housings, each sediment filter being mounted on a respective one of the filter housings.

2. The cartridge according to claim 1 , wherein the sediment filters are fluidly interposed between the extractant and reagent mixing chambers.

3. The cartridge according to claim 2 , wherein each processing train further includes a set of an upper slurry filtration chamber adjoining and in fluid communication with the extractant mixing chamber and a lower supernatant collection chamber arranged below the upper slurry filtration chamber and fluidly coupled to the reagent mixing chamber.

4. The cartridge according to claim 3 , wherein the sediment filter is interposed vertically between the upper slurry filtration chamber and the lower supernatant collection chamber.

5. The cartridge according to claim 3 , wherein each upper slurry filtration chamber includes an integrally formed sludge trap arranged radially outwards from the sediment filter to collect sludge removed from the slurry when the cartridge is centrifugated.

6. The cartridge according to claim 3 , wherein the lower supernatant collection chamber of each processing train are formed inside the filter housings of the annular filter ring.

7. The cartridge according to claim 6 , wherein each sediment filter is detachably mounted on top of a respective one of the filter housings.

8. The cartridge according to claim 3 , wherein each extractant mixing chamber is radially elongated and comprises an obliquely inclined outer wall defining an upward sloping surface leading into the upper slurry filtration chamber which is shallower in depth than the extractant mixing chamber to facilitate outward flow of the slurry into the upper slurry filtration chamber when the cartridge is centrifugated.

9. The cartridge according to claim 3 , wherein the extractant mixing chamber and upper slurry filtration chamber are each defined by and share a common recess formed in the main body of the cartridge.

10. The cartridge according to claim 9 , wherein the common recess gradually diverges moving radially outwards from the centerline axis such that an outer wall of the upper slurry filtration chamber is wider than a circumferential inner wall of the extractant mixing chamber.

11. The cartridge according to claim 3 , wherein when the cartridge is rotated by the rotary machine, the slurry flows radially outwards from the extractant mixing chamber into the upper slurry filtration chamber, downwards through the sediment filter into the lower supernatant collection chamber thereby producing the supernatant, and the supernatant flows circumferentially to the reagent mixing chamber.

12. The cartridge according to claim 3 , wherein the extractant mixing chamber, upper slurry filtration chamber, and lower supernatant collection chamber of each of the processing trains are radially aligned along a respective radial reference axis of each of the processing trains.

13. The cartridge according to claim 12 , wherein each reagent mixing chamber is circumferentially interspersed between each upper slurry filtration collection chamber and each lower supernatant collection chamber, respectively, and laterally offset from the radial reference axes of the processing trains.

14. The cartridge according to claim 1 , wherein each extractant mixing chamber is radially elongated and comprises a circumferential inner wall, and opposing obliquely inclined circumferential outer wall, and a pair of radial walls extending between the circumferential inner wall and the circumferential outer wall.

15. The cartridge according to claim 14 , wherein the radial walls are non-parallel and gradually diverge relative to each other moving outwards from the centerline axis forming a wedge shaped extractant chamber.

16. The cartridge according to claim 1 , further comprising a depressible extractant plunger assembly disposed in the extractant mixing chamber and containing a dosage of extractant, the extractant plunger assembly operable to dispense the dosage of extractant into the extractant mixing chamber by actuating the extractant plunger assembly.

17. The cartridge according to claim 1 , further comprising a depressible reagent plunger assembly disposed in the reagent mixing chamber and containing a dosage of a color changing reagent, the reagent plunger assembly operable to dispense the dosage of reagent into the reagent mixing chamber by actuating the reagent plunger assembly.

18. The cartridge according to claim 1 , wherein the reagent mixing chamber includes an associated reagent fill hole for introducing reagent into the reagent mixing chamber.

19. The cartridge according to claim 18 , wherein each reagent fill hole is fluidly connected to its respective reagent mixing chamber by a reagent injection conduit which extends radially between the reagent mixing chamber and the reagent fill hole.

20. The cartridge according to claim 1 , further comprising a top cover enclosing the extractant mixing chamber and the reagent mixing chamber.

21. The cartridge according to claim 20 , wherein the top cover is a discrete component sealingly coupled to the main body of the cartridge.

22. The cartridge according to claim 1 , wherein each of the processing trains are arranged around the centerline axis in sectors.

23. The cartridge according to claim 1 , wherein the main body has a shape that is one of circular, a polygon, a lemniscate, or a rose curve.

Assignees

• Prec Planting Llc

Inventors

• Swanson Todd
• Koch Dale
• Vaccari Adam

Classifications

• G01N33/245

  for agricultural purposes · CPC title

• G01N33/24

  Earth materials (G01N33/42 takes precedence) · CPC title

• B01L2400/0409

  centrifugal forces · CPC title

• B01L2300/0864

  comprising only one inlet and multiple receiving wells, e.g. for separation, splitting · CPC title

• B01L2300/0858

  Side walls · CPC title