Method for decreasing colorectal cancer cells

Patent metadata

Field	Value
Publication number	US-12090169-B2
Application number	US-202418599582-A
Country	US
Kind code	B2
Filing date	Mar 8, 2024
Priority date	Jan 19, 2022
Publication date	Sep 17, 2024
Grant date	Sep 17, 2024

Abstract

A method of making Cu—Ag 3 PO 4 nanoparticles is provided. The method includes forming a mixture of at least one silver salt, at least one phosphate salt, and at least one copper (II) salt. The method further includes dissolving the mixture in water. The method further includes sonicating the mixture. The method further includes precipitating the Cu—Ag 3 PO 4 nanoparticles or “nanoparticles”. The copper is present in the nanoparticles in an amount of 2 to 23 weight percent (wt. %) based on the total weight of the Cu—Ag 3 PO 4 . The nanoparticles of the present disclosure find application in treating cervical cancer, and colorectal cancer. The nanoparticles may also be used in photodegrading environmental pollutants.

First claim

The invention claimed is:

1. A method of decreasing an amount of colorectal cancer cells in a cell culture, comprising:
contacting a composition with the colorectal cancer cells and incubating in a CO 2 incubator,
wherein the composition comprises Cu—Ag 3 PO 4 substantially spherical nanoparticles having a mean particle size of 100-1,000 nm,
wherein an amount of the Cu—Ag 3 PO 4 nanoparticles is 5.0 to 95 μg per mL of the cell culture,
wherein the Cu—Ag 3 PO 4 nanoparticles have an IC 50 of 36-46 μg per mL of the cell culture,
wherein the colorectal cancer cells are HCT-116 cells,
wherein copper is present in the Cu—Ag 3 PO 4 nanoparticles in an amount of 10-15 weight percent (wt. %) based on the total weight of the Cu—Ag 3 PO 4 nanoparticles.

2. The method of claim 1 , wherein the contacting is for 48 hours.

3. The method of claim 2 , wherein the colorectal cancer cells are incubated in the CO2 incubator for 4 hours.

4. The method of claim 1 , wherein the Cu—Ag 3 PO 4 nanoparticles have a BET mean surface area of 4.2-6.1 m 2 /g.

5. The method of claim 4 , wherein the Cu—Ag 3 PO 4 nanoparticles have a BET mean pore size of 19.5-29.6 nm.

6. The method of claim 1 , wherein during the contacting the Cu—Ag 3 PO 4 nanoparticles are aggregated.

7. The method of claim 1 , wherein the composition consists of the Cu—Ag 3 PO 4 nanoparticles.

8. The method of claim 1 , wherein the Cu—Ag 3 PO 4 nanoparticles have a mean particle size of 200-300 nm.

9. The method of claim 1 , wherein the contacting is in vitro.

Assignees

• Univ Imam Abdulrahman Bin Faisal

Inventors

• Nawaz Muhammad
• Khan Firdos Alam

Classifications

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• C01P2006/14

  Pore volume · CPC title

• C01P2002/84

  by UV- or VIS- data · CPC title

• C01P2002/85

  by XPS, EDX or EDAX data · CPC title

• C01P2004/03

  obtained by SEM · CPC title