Systems and methods for multi-analysis

Patent metadata

Field	Value
Publication number	US-12085583-B2
Application number	US-202117319842-A
Country	US
Kind code	B2
Filing date	May 13, 2021
Priority date	Sep 25, 2011
Publication date	Sep 10, 2024
Grant date	Sep 10, 2024

Abstract

Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

First claim

What is claimed is:

1. A method of performing two or more assays for detecting a plurality of analytes in a biological sample from a subject, comprising:
receiving the biological sample in a biological sample processing system, wherein
the biological sample processing system comprises:
a sample handling system;
a plurality of assay units for performing said two or more assays to detect a plurality of analytes in the biological sample, and
at least two detection units;

receiving a cartridge in the biological sample processing system, the cartridge comprising a plurality of vessels;
executing, by a computer, a command to a controller to facilitate aliquoting of the two or more assays;
aliquoting at least a diluted portion of the sample into one of the plurality of assay units of the biological sample processing system while the cartridge remains inside the biological sample processing system;
decoupling one or more vessels from the cartridge while the cartridge remains inside the biological sample processing system to form one or more decoupled components;
using a centrifuge on one of said assay units;
performing in the biological sample processing system two or more assays using the plurality of assay units; and
detecting the plurality of analytes with at least one of a plurality of detection units in the biological sample processing system.

2. The method of claim 1 , wherein the biological sample is a blood sample.

3. The method of claim 2 , wherein the two or more assays are performed from a single blood sample obtained from the subject.

4. The method of claim 2 , wherein the blood sample is a plasma sample.

5. The method of claim 1 , wherein the biological sample processing system comprises at least three assay units and performs three or more assays at least partially in parallel, wherein the three or more assays comprise a nucleic acid amplification, an immunoassay, and a cytometric assay, and wherein the plurality of analytes comprise an antibody, an antigen, a nucleic acid, and a cell.

6. The method of claim 5 , wherein the biological sample processing system further comprises a cytometer.

7. The method of claim 1 , wherein the biological sample processing system further comprises a centrifuge.

8. The method of claim 1 , wherein the biological sample processing system further comprises a housing.

9. The method of claim 8 , wherein the cartridge comprises at least one thermal heating unit.

10. The method of claim 9 , wherein the cartridge further comprises at least one tip, and wherein a sample handling system of the biological sample processing system comprises at least one pipette nozzle configured to engage with the at least one tip.

11. The method of claim 1 , where the cartridge is provided in a refrigerated condition in a temperature in the range of about −20° ° C. to about 8° C. range for days to weeks.

12. The method of claim 11 , where the cartridge in the refrigerated condition has temperature in the range of about 2° ° C. to about 8° C. range for days to weeks.

13. The method of claim 11 , where the cartridge in the refrigerated condition has temperature in the range of about 0° ° C. to about 8° C. range for days to weeks.

14. The method of claim 1 , further comprising a heater mounted below a cartridge receiving location in the biological sample processing system.

15. The method of claim 14 , wherein the heater comprises thermoelectric device.

16. The method of claim 15 , wherein the heater further comprises a fan.

17. The method of claim 15 , wherein the heater further comprises a plurality of holes sized to engage portions of vessels in the cartridge.

18. The method of claim 15 , wherein the heater is configured to direct heat towards an underside of the cartridge when the cartridge is in a cartridge receiving location.

19. The method of claim 15 , wherein heater is configured to direct heat directly towards only a portion of an underside of the cartridge when the cartridge is in a cartridge receiving location.

Assignees

• Labrador Diagnostics Llc

Inventors

• Holmes Elizabeth A
• Chen Michael
• Ko Pey-Jiun

Classifications

• G01N33/92

  involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)} · CPC title

• G01N33/6827

  Total protein determination, e.g. albumin in urine · CPC title

• G01N33/62

  involving urea · CPC title

• C12Q1/52

  involving transaminase · CPC title

• C12Q1/42

  involving phosphatase · CPC title