Replaceable ground electrode for electrophysiology, electrode rejuvenating apparatus, and related methods and systems

Patent metadata

Field	Value
Publication number	US-12085559-B2
Application number	US-202117361860-A
Country	US
Kind code	B2
Filing date	Jun 29, 2021
Priority date	Jan 22, 2014
Publication date	Sep 10, 2024
Grant date	Sep 10, 2024

Abstract

A plenum assembly configured for electrophysiology assays, such as patch clamp techniques, includes one or more ground electrode assemblies. The ground electrode assemblies are individually removable from a plenum base of the plenum assembly in a non-destructive manner, and may be reinstalled in the plenum base in a manner that reestablishes electrical contact with ground circuitry without requiring soldering or other additional steps. A rejuvenating apparatus is provided for rejuvenating one or more ground electrode assemblies removed from the plenum base.

First claim

What is claimed is:

1. A method for rejuvenating a ground electrode assembly, the method comprising:
removing the ground electrode assembly from a mounted position in a plenum base, wherein the ground electrode assembly comprises a housing and a porous electrode positioned in the housing, and at the mounted position the ground electrode assembly is in a mounting receptacle of the plenum base and the electrode is in electrical contact with a ground circuit in the mounting receptacle, and wherein removing the ground electrode assembly comprises moving the electrode out of electrical contact with the ground circuit in a non-destructive manner;
threadably mating the ground electrode assembly with a vessel body defining an interior chamber, wherein the electrode is disposed proximate an outer surface of the vessel body and distal the interior chamber, and wherein the vessel body comprises a fluid port disposed opposite the electrode;
connecting the fluid port to a pressure-applying device;
immersing the ground electrode assembly in a rejuvenating fluid for a period of time; and
activating the pressure-applying device to perfuse the rejuvenating fluid through the electrode.

2. The method of claim 1 , comprising, after immersing, mounting the ground electrode assembly in the mounting receptacle such that the electrode makes electrical contact with the ground circuit.

3. The method of claim 1 , wherein the electrode comprises a top electrode surface, a bottom electrode surface, and an electrode thickness between the top electrode surface and the bottom electrode surface.

4. The method of claim 1 , wherein the electrode comprises a top electrode surface, a bottom electrode surface, and an electrode thickness between the top electrode surface and the bottom electrode surface, and wherein activating the pressure-applying device comprises applying a pressure differential across the electrode thickness to perfuse the rejuvenating fluid through the electrode thickness.

5. The method of claim 4 , wherein the electrode is positioned in the housing such that immersing the ground electrode assembly exposes at least a portion of the top electrode surface and at least a portion of the bottom electrode surface to the rejuvenating fluid.

Assignees

• Molecular Devices Llc

Inventors

• Smith Thomas L
• Verdonk Edward

Classifications

• G01N27/38

  Cleaning of electrodes · CPC title

• G01N33/48728 Primary

  Investigating individual cells, e.g. by patch clamp, voltage clamp (investigating individual particles in general G01N15/10) · CPC title