Adjusting separation method using sensor data and numerical analysis

Patent metadata

Field	Value
Publication number	US-12085545-B2
Application number	US-202117215872-A
Country	US
Kind code	B2
Filing date	Mar 29, 2021
Priority date	Mar 30, 2020
Publication date	Sep 10, 2024
Grant date	Sep 10, 2024

Abstract

A process of determining a modified separation method for a sample separation apparatus based on an initial separation method, including carrying out the initial separation method on a sample separation apparatus, detecting sensor data at the sample separation apparatus during carrying out the initial separation method, and carrying out a numerical analysis for determining the modified separation method by modifying at least one operation parameter of the initial separation method and by using the detected sensor data.

First claim

The invention claimed is:

1. A process of determining a modified separation method for a sample separation apparatus based on an initial separation method, the process comprising:
carrying out the initial separation method on a sample separation apparatus by flowing a mobile phase through the sample separation apparatus in a gradient mode wherein a composition of the mobile phase is varied over time, and injecting a fluidic sample into the mobile phase, wherein the sample separation apparatus separates different components of the fluidic sample while the fluidic sample flows with the mobile phase;
detecting sensor data at the sample separation apparatus during carrying out the initial separation method, wherein the sensor data is indicative of the composition of the mobile phase flowing through the sample separation apparatus during carrying out the initial separation method on the sample separation apparatus; and
carrying out a numerical analysis for determining the modified separation method by modifying at least one operation parameter of the initial separation method and by using the detected sensor data.

2. The process according to claim 1 , wherein the process comprises determining the initial separation method by:
receiving a target specification from a user being indicative of a target of a sample separation task;
searching for the initial separation method in a method database, which includes a plurality of reference separation methods; and
selecting the initial separation method from the reference separation methods based on the received target specification, or based on the received target specification in accordance with a best match with the received target specification.

3. The process according to claim 2 , wherein the process comprises:
searching in the method database for a plurality of candidate separation methods as candidates for the initial separation method;
selecting the candidate separation methods from the reference separation methods based on the received target specification;
carrying out each of the selected candidate separation methods on the sample separation apparatus; and
selecting one of the candidate separation methods as the initial separation method based on a match between a result of carrying out the candidate separation methods on the sample separation apparatus and the target specification.

4. The process according to claim 1 , wherein the process comprises one of:
carrying out the numerical analysis using a result of carrying out the initial separation method;
carrying out the numerical analysis using a result of carrying out the initial separation method, wherein the result comprises a chromatogram.

5. The process according to claim 1 , wherein the process comprises detecting sensor data indicative of at least one selected from the group consisting of:
a flow rate of the mobile phase flowing through the sample separation apparatus during carrying out the initial separation method on the sample separation apparatus;
a temperature of the mobile phase and/or the fluidic sample flowing through the sample separation apparatus during carrying out the initial separation method on the sample separation apparatus; and
a pressure of the mobile phase flowing through the sample separation apparatus during carrying out the initial separation method on the sample separation apparatus.

6. The process according to claim 1 , wherein the process comprises detecting the sensor data by at least one sensor located at a location according to at least one of:
upstream of a sample separation unit of the sample separation apparatus, wherein the sample separation unit is configured to separate the different components of the fluidic sample;
upstream of a sample separation unit of the sample separation apparatus, and between a fluid drive and an injector of the sample separation apparatus, wherein the sample separation unit is configured to separate the different components of the fluidic sample, the fluid drive is configured to drive the flowing of the mobile phase, and the injector is configured to inject the fluidic sample into the mobile phase;
between an injector and a sample separation unit of the sample separation apparatus, wherein the injector is configured to inject the fluidic sample into the mobile phase, and the sample separation unit is configured to separate the different components of the fluidic sample.

7. The process according to claim 1 , wherein the process comprises carrying out the numerical analysis using at least one selected from the group consisting of: a finite element method analysis; a finite difference method analysis; a boundary element method analysis; a control volume method analysis; and a random walk method analysis.

8. The process according to claim 1 , wherein the process comprises carrying out the initial separation method on the same sample separation apparatus on which the determined modified separation method is to be carried out subsequently.

9. The process according to claim 1 , wherein the process comprises carrying out once or multiple times the following sequence:
carrying out the modified separation method on the sample separation apparatus;
detecting further sensor data at the sample separation apparatus during carrying out the modified separation method; and
carrying out a further numerical analysis for determining a further modified separation method by modifying at least one operation parameter of the modified separation method and by using the detected further sensor data.

10. The process according to claim 1 , wherein the process comprises one of:
further modifying the modified separation method determined for the sample separation apparatus for transferring the modified separation method to another sample separation apparatus;
further modifying the modified separation method determined for the sample separation apparatus for transferring the modified separation method to another sample separation apparatus, under consideration of device-specific particularities of at least one of the sample separation apparatus and the other sample separation apparatus.

11. The process according to claim 1 , wherein the process comprises determining information indicative of a robustness of the modified separation method against variations of the at least one operation parameter and/or against variations of at least one other physical parameter.

12. The process according to claim 1 , wherein the process comprises modifying the initial separation method for enhancing robustness of the modified separation method against variations of the at least one operation parameter and/or against variations of at least one other physical parameter.

13. The process according to claim 1 , wherein the process comprises:
extracting at least one sample parameter being indicative of a property or behavior of the fluidic sample based on the initial separation method and the sensor data, or additionally based on a result of carrying out the initial separation method on the sample separation apparatus; and
determining the modified separation method under consideration of the extracted at least one sample parameter.

14. A non-transitory computer-readable medium with instructions stored thereon, that when executed by a processor, carry out or control the process according to claim 1 .

15. A program element for determining a modified separation method for a sample separation apparatus based on an initial separation method, wherein the program element, when being executed by one or a plurality of processors, is configured to carry out or control the process accordi

Assignees

• Agilent Technologies Inc

Inventors

• Schuhn Bettina
• Effelsberg Uwe

Classifications

• G01N30/16

  Injection (G01N30/24 takes precedence) · CPC title

• G01N2030/027

  Liquid chromatography · CPC title

• G01N30/34

  of fluid composition, e.g. gradient (G01N30/36 takes precedence) · CPC title

• G01N30/8658 Primary

  Optimising operation parameters · CPC title

• G01N30/86 Primary

  Signal analysis · CPC title