Apparatus and method for separating single cells

Patent metadata

Field	Value
Publication number	US-12085528-B2
Application number	US-201516311619-A
Country	US
Kind code	B2
Filing date	May 14, 2015
Priority date	Nov 13, 2014
Publication date	Sep 10, 2024
Grant date	Sep 10, 2024

Abstract

An apparatus and method for separating single cells. The apparatus includes: a fluid channel having an upper panel, a lower panel, and a flow path formed therebetween that is configured to convey a sample including a single cell; a single cell measuring unit including first and second electrodes provided on the fluid channel in a predetermined spaced relationship for applying electrical signals to the sample in the flow path, and a detection electrode provided between the first and second electrodes to detect the single cell in the sample in the flow path, such that the single cell measuring unit applies the electrical signals to the sample in the flow path, detects the electrical signals of the sample, and detects whether there is the single cell in the sample; and a single cell separation control device which outputs a single cell separation control signal when the single cell is detected by the detection electrode.

First claim

The invention claimed is:

1. A single cell separating apparatus comprising:
a fluid channel which has an upper panel and a lower panel and has a flow path which is formed between the upper panel and the lower panel and configured to convey a sample including a single cell;
a single cell measuring unit comprising an operative electrode pair comprising first and second electrodes provided along the fluid channel and spaced apart from each other at a predetermined interval in a direction of the flow path, said operative electrode pair being operative to work collectively to apply an electrical signal to the sample being conveyed through the flow path of the fluid channel, and a detection electrode which is provided between the first electrode and the second electrode in the direction of the flow path and detects the single cell in the sample being conveyed through the flow path, such that the single cell measuring unit applies a first electrical signal to the sample being conveyed through the flow path, detects a second electrical signal of the sample to which the first electrical signal is applied, and detects whether there is the single cell in the sample; and
a single cell separation control device which outputs a single cell separation control signal when the single cell is detected by the detection electrode.

2. The single cell separating apparatus of claim 1 , wherein the first electrode and the second electrode are provided on the upper panel, the lower panel, or the upper and lower panels of the fluid channel.

3. The single cell separating apparatus of claim 1 , wherein the single cell separation control device counts and provides the number of single cells passing through the flow path based on the second electrical signal inputted from the detection electrode.

4. The single cell separating apparatus of claim 1 , further comprising:
a buffer unit in fluid communication with the fluid channel, the buffer unit defining a flow path having a shape reducing a reverse flow caused by air pressure created when supplying the fluid to be coupled to the single cell.

5. The single cell separating apparatus of claim 4 , wherein the buffer unit has an elongated flow path between two points that is longer than a linear distance between said two points.

6. The single cell separating apparatus of claim 1 , further comprising:
a heterologous cell discharge channel which has one end portion connected to the fluid channel and discharges a heterologous cell from the single cell separating apparatus when the heterologous cell is detected in the fluid channel by the single cell measuring unit; and
a valve channel which has one end portion provided in the heterologous cell discharge channel and the other end portion connected to an air injecting unit for injecting air into the heterologous cell discharge channel.

7. The single cell separating apparatus of claim 6 , wherein the fluid channel and the heterologous cell discharge channel have different tube thicknesses.

8. The single cell separating apparatus of claim 6 , wherein a PDMS membrane is formed between the heterologous cell discharge channel and the valve channel.

9. The single cell separating apparatus of claim 1 , wherein the well plate has multiple spaces, a first space stores heterologous cells, and the remaining multiple spaces separate and store the single cells, one space for each single cell.

10. A single cell separating apparatus comprising:
a fluid channel having an upper panel and a lower panel cooperating to define a flow path configured to convey a sample including a single cell;
a single cell measuring unit comprising:
an electrode pair comprising first and second electrodes provided adjacent the fluid channel, said first and second electrodes being provided to be spaced apart from each other at a predetermined interval in a direction of the flow path, said first and second electrodes being operatively coupled and controlled to operate to work collectively to apply a particular electrical signal to the sample conveyed through the flow path of the fluid channel to create an electrical field; and
a detection electrode provided adjacent the fluid channel at a location between the first electrode and the second electrode in the direction of the flow path, said detection electrode being operable to detect changes in the electrical field as the single cell in as the sample is conveyed through the flow path, such that the single cell measuring unit applies the electrical field to the sample being conveyed through the flow path, detects another electrical signal corresponding to changes in the electrical field of the sample as it is conveyed though the flow path, and thereby detects whether there is the single cell in the sample in the flow path; and

a single cell separation control device operable to output a single cell separation control signal when the single cell is detected by the detection electrode.

11. A single cell separating apparatus comprising:
a fluid channel defining a flow path configured to convey a sample including a single cell;
a single cell measuring unit comprising:
an operative electrode pair having first and second electrodes provided adjacent the fluid channel, said first and second electrodes being provided to be spaced apart from each other at a predetermined interval in a direction of the flow path, said first and second electrodes of said operative electrode pair being operatively coupled and configured to work collectively as a single working electrode pair to collectively apply a first electrical signal as the sample is conveyed through the flow path of the fluid channel; and
a detection electrode provided adjacent the fluid channel at a location between the first electrode and the second electrode in the direction of the flow path, said detection electrode being operable to detect a second electrical signal associated with the sample in response to the sample passing said first electrode; and

a single cell separation control device operable to output a single cell separation control signal when the single cell is detected by the detection electrode.

Assignees

• Univ Inje Ind Acad Coop Found
• Inje Univ Industry—Academic Cooperation Foundation

Inventors

• Han Ki-Ho
• Kim Jin-Ho

Classifications

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• B01L3/502715 Primary

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• G01N2015/1029

  Particle size · CPC title

• G01N2015/1024

  Counting particles by non-optical means · CPC title