Interposer with first and second adhesive layers

Patent metadata

Field	Value
Publication number	US-12083514-B2
Application number	US-202217727622-A
Country	US
Kind code	B2
Filing date	Apr 22, 2022
Priority date	Jul 3, 2018
Publication date	Sep 10, 2024
Grant date	Sep 10, 2024

Abstract

An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

First claim

What is claimed is:

1. A flow cell comprising:
a first substrate having a surface in which a plurality of wells are defined, wherein a biological probe is disposed in each of the wells;
a second substrate; and
an interposer disposed in-between the first substrate and the second substrate, the interposer comprising:
a base layer having a first surface and a second surface opposite the first surface, the base layer comprising black polyethylene terephthalate (PET),
a first adhesive layer bonding the first surface of the base layer to a surface of the first substrate, the first adhesive layer comprising acrylic adhesive, and
a second adhesive layer bonding the second surface of the base layer to a surface of the second substrate, the second adhesive layer comprising acrylic adhesive, wherein:

a plurality of microfluidic channels are disposed within the interposer, the plurality of microfluidic channels extending through each of the base layer, the first adhesive layer, and the second adhesive layer, the plurality of microfluidic channels being configured to deliver a fluid to the plurality of wells.

2. The flow cell of claim 1 , wherein each of the first and second substrates comprises glass, and wherein a bond between each of the first and second adhesive layers and the respective surfaces of the first and second substrates is adapted to withstand a shear stress of greater than about 50 N/cm2 and a peel force of greater than about 1 N/cm.

3. The flow cell of claim 1 , wherein;
each of the first and second substrates comprises a resin layer that is less than about one micron thick,
the resin layer of the first substrate includes the surface that is bonded to the first adhesive,
the resin layer of the second substrate includes the surface that is bonded to the second adhesive layer, and
a bond between each of the resin layers and the respective first and second adhesive layers is adapted to withstand a shear stress of greater than about 50 N/cm2 and a peel force of greater than about 1 N/cm.

4. The flow cell of claim 1 , wherein a total thickness of the base layer, first adhesive layer, and second adhesive layer is in a range of about 1 to about 200 microns.

5. The flow cell of claim 1 , wherein the base layer has a thickness in a range of about 10 to about 100 microns, and each of the first adhesive layer and the second adhesive layer has a thickness in a range of about 5 to about 50 microns.

6. The flow cell of claim 1 , wherein the each of the first and second adhesive layers has an auto-fluorescence in response to a 532 nm excitation wavelength of less than about 0.25 a.u. relative to a 532 nm fluorescence standard.

7. The flow cell of claim 6 , wherein the each of the first and second adhesive layers has an auto-fluorescence in response to a 635 nm excitation wavelength of less than about 0.15 a.u. relative to a 635 nm fluorescence standard.

8. The flow cell of claim 1 , wherein the base layer comprises at least about 50% black PET.

9. The flow cell of claim 1 , wherein the base layer consists essentially of black PET.

10. The flow cell of claim 1 , wherein each of the first and second adhesive layers comprises at least about 5% acrylic adhesive.

11. The flow cell of claim 1 , wherein each of the first and second adhesive layers consists essentially of acrylic adhesive.

Assignees

• Illumina Inc

Inventors

• Zimmerley Maxwell
• Qiang Liangliang
• Bowen M Shane
• Modiano Steven H
• Yuan Dajun
• Smith Randall
• Pitera Arthur J
• Tran Hai Quang
• Kreindl Gerald

Classifications

• B01L3/502746

  characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves · CPC title

• B01L2200/0642

  Filling fluids into wells by specific techniques · CPC title

• B01L3/502715 Primary

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01L3/56

  Labware specially adapted for transferring fluids · CPC title

• B01L3/502707 Primary

  characterised by the manufacture of the container or its components · CPC title