Bioartificial ultrafiltration device and methods related thereto

Patent metadata

Field	Value
Publication number	US-12076470-B2
Application number	US-202318143005-A
Country	US
Kind code	B2
Filing date	May 3, 2023
Priority date	Mar 7, 2016
Publication date	Sep 3, 2024
Grant date	Sep 3, 2024

Abstract

Bioartificial ultrafiltration devices comprising a scaffold comprising a population of cells enclosed in a matrix and disposed adjacent a plurality of channels are provided. The population of cells provides molecules such as therapeutic molecules to a subject in need thereof and is supported by the nutrients filtered in an ultrafiltrate from the blood of the subject. The plurality of channels in the scaffold facilitate the transportation of the ultrafiltrate and exchange of molecules between the ultrafiltrate and the population of cells.

First claim

The invention claimed is:

1. A system comprising:
(A) an ultrafiltration device comprising:
a first planar scaffold and a second planar scaffold each comprising a matrix comprising a plurality of channels extending from a first surface to a second surface of each of the planar scaffolds;
a first semipermeable ultrafiltration membrane disposed on the first surface of the first and second planar scaffolds;
a first compartment adjacent to and sandwiched between the first surface of the first and second planar scaffolds and comprising an inlet and an outlet, wherein the first semipermeable ultrafiltration membrane allows transport of ultrafiltrate from the first compartment to the scaffolds;
a second compartment adjacent to the second surface of the first planar scaffold and comprising an inlet and an outlet; and
a third compartment adjacent to the second surface of the second planar scaffold and comprising an inlet and an outlet;
wherein the first semipermeable ultrafiltration membrane comprises a plurality of pores, and
wherein the ultrafiltrate traverses from the plurality of channels in the scaffolds into the second compartment and the third compartment; and
(B) a housing that contains the ultrafiltration device and comprises:
a blood inlet port operably connected to a first end of the first compartment;
a blood outlet port operably connected to second end of the first compartment;
an ultrafiltrate outlet operably connected to the second compartment and third compartment.

2. The system of claim 1 , wherein the first planar scaffold further comprises a population of cells adjacent to the plurality of channels.

3. The system of claim 2 , wherein the cells are insulin producing cells.

4. The system of claim 3 , wherein the insulin producing cells are derived from differentiation of stem cells.

5. The system of claim 3 , wherein the insulin producing cells are pancreatic cells isolated from pancreatic islets.

6. The system of claim 2 , wherein the system is located inside a subject and the cells are autologous to a subject.

7. The system of claim 1 , wherein the second planar scaffold further comprises a second population of cells adjacent to the plurality of channels.

8. The system of claim 1 , wherein the first semipermeable ultrafiltration membrane has pores with a width in the range of 5 nm to 5 μm.

9. The system of claim 8 , wherein the first semipermeable ultrafiltration membrane has pores with a width in the range of 0.1 μm to 2 μm.

10. The system of claim 1 , wherein the second semipermeable ultrafiltration membrane has pores with a width in the range of 5 nm to 5 μm.

11. The system of claim 10 , wherein the second semipermeable ultrafiltration membrane has pores with a width in the range of 0.1 μm to 2 μm.

12. The system of claim 1 , wherein the second semipermeable ultrafiltration membrane comprises a plurality of pores having a width larger than the width of the plurality of pores in the first semipermeable ultrafiltration membrane.

13. The system of claim 1 , wherein the thickness of the first semipermeable ultrafiltration membrane is in the range of 0.1 μm to 1000 μum.

14. The system of claim 13 , wherein the thickness of the first semipermeable ultrafiltration membrane is in the range of 200 μm to 1000 μm.

15. The system of claim 1 , wherein the thickness of the second semipermeable ultrafiltration membrane is in the range of 0.1 μm to 1000 μm.

16. The system of claim 14 , wherein the thickness of the second semipermeable ultrafiltration membrane is in the range of 200 μm to 1000 μm.

17. The system of claim 1 , wherein the surface area of the first semipermeable ultrafiltration membrane is in the range of 1 cm 2 to 100 cm 2 .

18. The system of claim 17 , wherein the surface area of the first semipermeable ultrafiltration membrane is in the range of 15 cm 2 to 30 cm 2 .

19. The system of claim 1 , wherein the surface area of the second semipermeable ultrafiltration membrane is in the range of 1 cm 2 to 100 cm 2 .

20. The system of claim 19 , wherein the surface area of the second semipermeable ultrafiltration membrane is in the range of 15 cm 2 to 30 cm 2 .

Assignees

• Univ California

Inventors

• Roy Shuvo
• Kant Rishi
• Posselt Andrew
• Song Shang
• Moses Willieford
• Blaha Charles

Classifications

• B01D61/145

  Ultrafiltration · CPC title

• A61M2202/09

  Body tissue · CPC title

• A61B5/150992

  Blood sampling from a fluid line external to a patient, such as a catheter line, combined with an infusion line; Blood sampling from indwelling needle sets, e.g. sealable ports, luer couplings or valves · CPC title

• B01D67/0037

  by deposition from the gaseous phase, e.g. CVD, PVD · CPC title

• B01D69/08

  Hollow fibre membranes (manufacture of hollow fibres D01D5/24, D01F1/08) · CPC title