Insecticidal proteins from plants and methods for their use

Patent metadata

Field	Value
Publication number	US-12071630-B2
Application number	US-202117483973-A
Country	US
Kind code	B2
Filing date	Sep 24, 2021
Priority date	Jul 1, 2016
Publication date	Aug 27, 2024
Grant date	Aug 27, 2024

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

First claim

That which is claimed is:

1. A DNA construct comprising a polynucleotide sequence encoding an insecticidal polypeptide, wherein the polypeptide has at least 70% sequence identity to SEQ ID NO: 2, wherein the polynucleotide sequence is operably linked to a heterologous regulatory element.

2. The DNA construct of claim 1 , wherein the polypeptide sequence has at least 80% sequence identity to SEQ ID NO: 2.

3. A transgenic plant comprising the DNA construct of claim 1 .

4. A method for controlling pest infestation, the method comprising providing in the diet of the pest the transgenic plant of claim 3 or a part thereof.

5. A method for improving the yield of a crop, the method comprising growing the transgenic plant of claim 3 , wherein the yield of the crop is increased in the presence of an insect pest relative to the crop not comprising said transgenic plant.

6. The method of claim 4 or 5 , wherein the insect pest is resistant to at least one Cry insecticidal protein.

Assignees

• Pioneer Hi Bred Int

Inventors

• Barry Jennifer Kara
• Clark Catherine J
• Gerber Ryan Michael
• Lum Amy
• Mathis John P
• Ong Azalea S
• Peterson-Burch Brooke
• Wolfe Thomas Chad
• Xie Weiping
• Yalpani Nasser
• Zhong Xiaohong

Classifications

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C07K14/415

  from plants · CPC title

• A01N37/46

  N-acyl derivatives · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title