System and method for continuous microwave-assisted extraction of bioactive agents from biomass

Patent metadata

Field	Value
Publication number	US-12070052-B2
Application number	US-202217865120-A
Country	US
Kind code	B2
Filing date	Jul 14, 2022
Priority date	Dec 5, 2016
Publication date	Aug 27, 2024
Grant date	Aug 27, 2024

Abstract

The presently disclosed subject matter is directed to a method of extracting bioactive agents from biomass using continuous microwave-assisted extraction. The disclosed method includes contacting the biomass with a solvent and optionally homogenizing the biomass. Then blending the biomass and the solvent and continuously passing the biomass though one more microwave assisted extraction units, thereby exposing the biomass to microwave energy. The solvent is separated from the biomass, where the separated solvent is the bioactive agent.

First claim

What is claimed is:

1. A method of extracting one or more bioactive agents from biomass, the method comprising:
blending the biomass and the one or more bioactive agents;
continuously passing the biomass though one or more microwave assisted extraction units, thereby exposing the biomass to microwave energy; and
separating the one or more bioactive agents from the biomass using the one or more microwave assisted extraction units that use the polarity of water in the subcritical range in lieu of chemical solvents to extract the one or more bioactive agents from the biomass.

2. The method of claim 1 , wherein the one or more microwave assisted extraction units are constructed from rigid microwave transparent materials selected from ceramics, glass, TEFLON®, polyetherimide, polymethylpentene, polyetheretherketone, and combinations thereof.

3. The method of claim 1 , wherein each microwave assisted extraction unit comprises at least one cylindrical, traveling wave, or modular microwave flow treatment devices.

4. The method of claim 1 , wherein the one or more bioactive agents is water, acetic acid, innate liquid, ethanol, or combinations thereof.

5. The method of claim 1 , wherein the ratio of solvent the one or more bioactive agents to biomass is about 1:10, 1:5, 1:4, 1:3, 1:2, or 1:1.

6. The method of claim 1 , wherein the one or more bioactive agents are selected from one or more carotenoids, polyphenols, vitamins, flavonoids, isoflavones, curcuminoids, ceramides, proanthocyanidins, terpenoids, sterols, phytosterols, sterol esters, tocotrienols, squalenes, or retinoids.

7. The method of claim 1 , wherein the biomass comprises agricultural crop wastes, crop residues, or combinations thereof.

8. The method of claim 1 , wherein the separating comprises gravity filtration, vacuum filtration, syringe filtration, passing through a resin, or combinations thereof.

9. The method of claim 1 , further comprising concentrating the one or more bioactive agents.

10. The method of claim 1 , wherein one or more additives are added to the biomass before, after, or both before and after passing the biomass though a microwave assisted extraction module.

11. The method of claim 10 , wherein the one or more additives are selected from acids, bases, salts, buffering systems, chelating agents, antioxidants, antimicrobial agents, or combinations thereof.

12. The method of claim 1 , further comprising homogenizing the biomass.

13. A continuous microwave assisted extraction method comprising:
extracting one or more bioactive agents from biomass at one or more flow-through microwave processing units in lieu of chemical solvents, wherein at least one of the one or more flow-through microwave processing units is a subcritical water microwave assisted extraction unit that uses a polarity of water in a subcritical range, wherein the extraction system is configured to provide for continuous extraction.

14. The method of claim 13 , extracting the one or more bioactive agents from the biomass includes exposing the biomass material to a temperature of about 120-190° C. and a pressure of about 50-200 psi.

15. The method of claim 13 , wherein each flow-through microwave processing unit comprises at least one of: cylindrical, traveling wave, or modular microwave flow treatment devices.

16. The method of claim 13 , wherein the flow-through microwave processing units are constructed from rigid microwave transparent materials selected from ceramics, glass, TEFLON®, polyetherimide, polymethylpentene, polyetheretherketone, and combinations thereof.

17. The method of claim 13 , wherein the flow-through microwave processing units comprise at least one microwave energy generator.

18. The method of claim 13 , further comprising testing pH, concentration, temperature, or combinations thereof.

Assignees

• Sinnovatek Inc
• Univ North Carolina State

Inventors

• Druga Michael
• Vargochik Amanda
• Simunovic Josip

Classifications

• B01F23/804

  Drying the mixture · CPC title

• B01F23/81

  Grinding the mixture · CPC title

• H05B6/78

  Arrangements for continuous movement of material · CPC title

• H05B6/6402

  Aspects relating to the microwave cavity · CPC title

• H05B6/645

  using temperature sensors · CPC title