Image analysis and measurement of biological samples

Patent metadata

Field	Value
Publication number	US-12066440-B2
Application number	US-202017082979-A
Country	US
Kind code	B2
Filing date	Oct 28, 2020
Priority date	Jul 25, 2012
Publication date	Aug 20, 2024
Grant date	Aug 20, 2024

Abstract

Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples.

First claim

What is claimed is:

1. A system for imaging a sample, the system comprising:
a stage configured to receive a sample vessel holder, the stage having an optically transmissive surface;
a sample holder containing said sample, the sample holder comprising a cuvette, the cuvette comprising an optically transmissive surface of the cuvette and a reflective surface of the cuvette,
wherein the optically transmissive surface and the reflective surface are each configured such that light passing through the optically transmissive surface simultaneously provides both epi-illumination and trans-illumination to the sample in the sample chamber,
wherein the epi-illumination comprises a light traveling from said illumination source to the sample without reflection at a surface of the optically transmissive material, and
wherein the trans-illumination comprises the light traveling within the optically transmissive material and to the sample following at least one reflection from at least one surface of said optically transmissive material; and

a light source for illuminating formed components in the sample through the stage,
wherein the sample holder comprises an interface surface configured to engage the optically transparent surface of the sample holder receiver whereby the interface surface conforms to the optically transparent surface without significant distortion of the light passing through the interface surface.

2. The system of claim 1 , wherein the interface surface of the sample holder is formed from a polymer material.

3. The system of claim 1 , wherein the interface surface of the sample holder is formed of a material softer than a material used to form the optically transparent surface of the sample holder receiver.

4. The system of claim 1 , further comprising a compression unit for applying pressure to conform the interface surface to a shape configured to conform with the optically transparent surface of the sample holder receiver.

5. The system of claim 1 , further comprising a handling unit configured to be coupled to the sample holder to facilitate transport of sample holder on and off the stage, and increase mechanical rigidity of the sample holder.

6. The system of claim 1 , further comprising an opaque handling unit configured to be coupled to the sample holder.

7. The system of claim 1 , where all imaging of the sample is configured to occur without passing light in a substantially straight line through a first surface opposite a second surface, the first surface closer to the light source than the second source, to a detector.

8. The system of claim 1 , where the light source is not located on a first one side of the sample holder to deliver light to a detector on a second side opposite the first side of the sample holder, the first side closer to the light source than the second source.

9. The system of claim 1 , wherein the sample holder comprises at least one concave or convex structure to provide a pathway for internally reflected light within the sample holder.

10. The system of claim 1 , wherein the sample holder comprises at least one concave or convex structure that comprises a surface configured to reflect light within the cuvette.

Assignees

• Labrador Diagnostics Llc

Inventors

• Pangarkar Chinmay
• Mohan Karan
• Wasson James R

Classifications

• G02B21/365

  Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence) · CPC title

• G02B21/16

  adapted for ultraviolet illumination {; Fluorescence microscopes (G02B21/0076 takes precedence)} · CPC title

• G01N2333/70596

  Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705 · CPC title

• G01N2333/70589

  CD45 · CPC title

• G01N2021/1738

  Optionally different kinds of measurements; Method being valid for different kinds of measurement · CPC title