Chromatography column and method of assembling the same

Patent metadata

Field	Value
Publication number	US-12066413-B2
Application number	US-201917294710-A
Country	US
Kind code	B2
Filing date	Dec 4, 2019
Priority date	Dec 7, 2018
Publication date	Aug 20, 2024
Grant date	Aug 20, 2024

Abstract

Disclosed is a chromatographic separation column assembly ( 100 ) comprising a column cylinder ( 120 ) having a cylinder wall ( 126 ) including an inner wall surface ( 124 ) partially defining a column volume ( 50 ), and a column component ( 110/130 ) insertable into to the cylinder ( 120 ), wherein at least an edge region ( 113/133 ) of the column component is intended to be in contact or adjacent a part of the inner wall surface ( 124 ) in use, the edge region and the contacting or adjacent part of inner wall surface each being formed from a compatible heat fusible material or materials, and wherein at least the cylinder wall in the area of the contacting or adjacent part of the inner wall is formed from a material which allows transmission of the light energy needed to cause said fusing of the adapter plate edge region to the inner wall surface.

First claim

The invention claimed is:

1. A chromatographic separation column assembly comprising:
a column cylinder having a cylinder wall including an inner wall surface partially defining a column volume, and
a first column component comprising an adapter plate having a sliding seal disposed therearound and a second column component comprising a base, wherein both the first column component and the second column component are insertable into to the column cylinder,
wherein at least an adapter plate edge region and a base edge region of the are intended to be in contact or adjacent a part of the inner wall surface in use, the adapter plate and base edge regions and the contacting or adjacent part of the inner wall surface each being formed from a compatible heat fusible material or materials, and
wherein at least the cylinder wall in the area of the contacting or adjacent part of the inner wall surface is formed from a material which allows transmission of light energy needed to cause said fusing of the adapter plate edge region and the base edge region to the inner wall surface.

2. The column assembly as claimed in claim 1 , wherein light energy is supplied by a laser, of sufficient power to cause said heat fusion.

3. The column assembly as claimed in claim 1 , wherein the column cylinder wall is, at least at the area of the contacting or adjacent part of the inner wall surface, formed from an amorphous or otherwise generally light transmissive thermoplastic material which allows at least a portion of light energy to propagate through the column cylinder wall for causing said heat fusion.

4. The column assembly as claimed in claim 1 , wherein, a further component is formed, at least at an edge region, from a crystalline, semi crystalline, colored, filled, or coated thermoplastic, having properties which allow absorption of at least a substantial proportion of any light energy transmitted through the column cylinder wall.

5. The column assembly as claimed in claim 3 , wherein the amorphous or otherwise generally light transmissive thermoplastic material of the column cylinder is a PMMA.

6. The column assembly as claimed in claim 1 , wherein the column cylinder further includes an inner cylinder liner.

7. A method for fusing a component of a chromatography column assembly inside a chromatography column, the method comprising the steps of:
a) providing a chromatography column cylinder having a wall including an inner surface, the wall being formed at least partially from thermoplastics material which is at least partially light energy transmissive and heat fusible, and partially defines a column volume;
b) providing a first column component comprising an adapter plate having a sliding seal disposed therearound and a second column component comprising a base inside the column cylinder;
c) causing light energy to propagate through the light energy transmissive material of the wall toward the first column component and the second column component, the light energy being of sufficient magnitude to heat-fuse the heat fusible material of the first column component and the second column component to the heat fusible material of the wall at a melt area where the propagation path of the light meets respective edge regions of the first column component and the second column component;
d) moving the propagation path relative to the wall and the first column component and the second column component; and
e) continuing step c) during said movement resulting from step d), or repeating step c) at further locations of the melt area.

8. The method as claimed in claim 7 , wherein step e) is continued to provide a continuous seam of heat fused materials around the column cylinder, or is repeated to provide intermittently fused material.

9. The method as claimed in claim 7 , wherein the light energy is laser light energy which is supplied either continuously, as a pulse, or a repeating pulse.

10. The method as claimed in claim 7 , further including the step of at least partially filling the column volume with chromatography media, compressing the media with the component and preforming steps c), d) and e) whilst said the compressing is at least partially maintained.

11. The method as claimed in claim 10 , wherein the compressing is released once the fusing steps are completed.

12. The chromatography column assembly according to claim 1 , wherein the chromatography column assembly is pre-packed and disposable.

13. A chromatography column component fusing apparatus for manufacturing a chromatographic separation column assembly according to claim 1 , the fusing apparatus comprising a frame including a chromatography column cylinder support table, and a support for the first column component of the chromatography column insertable within the column cylinder, the apparatus further including a laser light generator for providing light energy directable to propagate through a wall of the column cylinder toward a first column component edge region and a second column component edge region, and of sufficient magnitude to cause heat fusing of the edge with an area of the inner wall surface of the column cylinder which is contacting or is adjacent to said edge region.

14. The assembly as claimed in claim 13 , wherein the apparatus further including an actuator for manipulating said adapter plate into the column cylinder, and for compressing chromatography media disposed within the volume of the column cylinder.

15. The apparatus as claimed in claim 13 , wherein said support includes a turntable, and wherein the turntable, the actuator and laser light source are controlled by a controller to provide continuous or intermittent fusing of the adapter plate to the inner surface of the column cylinder around an axis of rotation of the turntable.

Assignees

• Cytiva Sweden Ab

Inventors

• Salomonsson Daniel
• Lundkvist Mats

Classifications

• B01D15/22 Primary

  relating to the construction of the column · CPC title

• B01D15/206

  Packing or coating · CPC title

• G01N30/603

  retaining the stationary phase, e.g. Frits · CPC title

• G01N30/6091

  Cartridges · CPC title

• G01N30/6082 Primary

  transparent to radiation · CPC title