Sterile mutant and two line breeding system

Patent metadata

Field	Value
Publication number	US-12065658-B2
Application number	US-201916959928-A
Country	US
Kind code	B2
Filing date	Jan 3, 2019
Priority date	Jan 3, 2018
Publication date	Aug 20, 2024
Grant date	Aug 20, 2024

Abstract

Disclosed herein are nuclear male sterile sorghum mutants, three-component genetic constructs, and methods of using said mutants and genetic constructs, such as in a two-line male sterility system for hybrid breeding.

First claim

What is claimed is:

1. A Sorghum bicolor seed designated as MS8, wherein a sample of said seed has been deposited as ATCC Patent Deposit No. PTA-127606.

2. A plant, or a part thereof, produced by growing the seed of claim 1 .

3. A Sorghum bicolor plant having all the physiological and morphological characteristics of the plant of claim 2 .

4. A tissue culture of regenerable cells from the plant, or the part thereof, of claim 2 .

5. The tissue culture of regenerable cells of claim 4 , wherein the regenerable cells are selected from the group consisting of protoplasts and calli, and wherein the regenerable cells are from a leaf, pollen, ovule, cotyledon, hypocotyl, embryo, root, pod, flower, shoot or stalk.

6. A protoplast produced from the tissue culture of claim 4 .

7. A Sorghum bicolor plant regenerated from the tissue culture of claim 4 , wherein the plant has all of the physiological and morphological characteristics of a plant produced by growing a seed designated MS8 and deposited under ATCC Patent Deposit No. PTA-127606.

8. A tissue culture of regenerable cells from the plant, or the part thereof, of claim 7 .

9. The tissue culture of claim 8 , wherein said regenerable cells are selected from the group consisting of protoplasts and calli, and wherein the regenerable cells are from a leaf, pollen, ovule, cotyledon, hypocotyl, embryo, root, pod, flower, shoot or stalk.

10. A protoplast produced from the tissue culture of claim 9 .

11. A method for producing a Sorghum bicolor mutant MS8-derived Sorghum bicolor L. Moench plant, comprising: (a) crossing MS8 plants grown from MS8 seed, representative seed of which has been deposited under ATCC Patent Deposit No. PTA-127606, with a second Sorghum bicolor plant to yield progeny Sorghum bicolor seed; and (b) growing the progeny seed to yield a Sorghum bicolor mutant MS8-derived Sorghum bicolor plant.

12. The method of claim 11 , further comprising: (c) crossing the Sorghum bicolor mutant MS8-derived Sorghum bicolor plant of (b) with itself or a third Sorghum bicolor plant to yield a second Sorghum bicolor MS8-derived Sorghum bicolor progeny seed; and (d) growing the second Sorghum bicolor progeny seed of (c) to yield a second Sorghum bicolor mutant MS8-derived Sorghum bicolor plant.

13. The method of claim 12 , wherein (c) and (d) are repeated at least one time to generate an additional Sorghum bicolor mutant MS8-derived Sorghum bicolor plant.

14. A method of introducing a desired trait into Sorghum bicolor MS8 comprising:
(a) crossing MS8 plants grown from MS8 seed, representative seed of which has been deposited under ATCC Patent Deposit No. PTA-127606, with plants of a second Sorghum bicolor mutant that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, site-specific recombination, increased transformability, abiotic stress tolerance, herbicide resistance, insect resistance, disease resistance, altered phosphorus, altered antioxidants, altered fatty acids, altered essential amino acids and altered carbohydrates;
(b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants;
(c) crossing the selected progeny plants with the MS8 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of Sorghum bicolor mutant MS8 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all the physiological and morphological characteristics of Sorghum bicolor mutant MS8.

15. A plant produced by the method of claim 14 , wherein the plant has the desired trait and all of the physiological and all morphological characteristics of said Sorghum bicolor mutant MS8.

16. A method for producing a Sorghum bicolor mutant plant having an altered agronomic trait comprising introducing a polynucleotide into a MS8 plant grown from MS8 seed, representative seed of which has been deposited under ATCC Patent Deposit No. PTA-127606, wherein the polynucleotide confers an altered agronomic trait to the plant selected from the group consisting of insect resistance, disease resistance, herbicide resistance, abiotic stress resistance, fatty acid metabolism, starch metabolism, carbohydrate metabolism, amino acid metabolism, phosphorus metabolism, antioxidant metabolism, male sterility, site specific DNA integration, plant growth, forage yield and flowering, and wherein the polynucleotide is expressed in the plant.

17. A Sorghum bicolor mutant plant produced by the method of claim 16 , wherein the plant has the altered agronomic trait and all of the physiological and all morphological characteristics of said Sorghum bicolor mutant MS8.

Assignees

• Uwm Res Foundation Inc
• Us Secretary Of Agriculture

Inventors

• Zhao Dazhong
• Xin Zhanguo

Classifications

• A01H6/4666

  Sorghum, e.g. sudangrass · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8289 Primary

  Male sterility · CPC title