Cell preservation or culturing arrangement

Patent metadata

Field	Value
Publication number	US-12065633-B2
Application number	US-201816756231-A
Country	US
Kind code	B2
Filing date	Oct 17, 2018
Priority date	Oct 19, 2017
Publication date	Aug 20, 2024
Grant date	Aug 20, 2024

Abstract

A biological cell preservation or culturing arrangement ( 20 ) comprises a chamber defining a fluid retaining space ( 30 ) for retaining in use a body of fluid ( 34 ) and a deformable membrane ( 36 ) in communication with the fluid retaining space, and being manipulable by an electroactive polymer actuator arrangement ( 38 ) to undergo a defined topology change to induce in the fluid a pattern of fluid flow by which fluid is exchanged between a sub-region ( 46 ) immediately proximal the deformable membrane and a sub-region ( 48 ) removed from the deformable membrane.

First claim

The invention claimed is:

1. A biological cell preservation arrangement comprising:
a chamber being a fluidically closed fluid retaining space configured to retain a volume of fluid and retain biological cells in the volume of fluid;
an impermeable deformable membrane within the chamber, and
an electroactive polymer actuator arrangement configured to deform the deformable membrane, and
a controller that controls the electroactive polymer actuator arrangement to change in a surface topology of the deformable membrane,
wherein the controller changes the surface topology in a sequence of topology changes to produce a flow of the fluid in a direction that corresponds to a direction of the sequence of topology changes and is parallel to a plane of the deformable membrane.

2. The biological cell preservation arrangement of claim 1 , wherein the controller is configured to effect regular or recurrent changes in the surface topology of the membrane on a continuous basis.

3. The biological cell preservation arrangement of claim 1 , wherein the deformable membrane at least partially bounds the fluid retaining space.

4. The biological cell preservation arrangement of claim 1 , wherein each surface topology change comprises surface deformations of the deformable membrane directed inward and outward of the fluid retaining space, the inward deformations being balanced volumetrically with the outward deformations such that there is a zero net change in the volume of the fluid retaining space before and after each topology change.

5. The biological cell preservation arrangement of claim 1 , wherein the electroactive polymer actuator arrangement comprises an array of actuator elements, each comprising an electroactive polymer that is actuable to undergo a bending action.

6. The biological cell preservation arrangement of claim 5 , wherein
the electroactive polymer actuator arrangement comprises an actuating layer, the actuating layer comprising the array of actuator elements, and
the electroactive polymer actuator arrangement comprises a layer of electroactive polymer and wherein each actuator element comprises a segment of the layer of electroactive polymer.

7. The biological cell preservation arrangement of claim 5 , wherein each actuator element is electrically drivable to move between a first stable actuation position and a second stable actuation position.

8. The biological cell preservation arrangement of claim 1 , wherein
the arrangement is a cell culturing arrangement, and
the pattern of fluid flow is for enabling nutrient replenishment of cells located at or proximal to the deformable membrane.

9. The biological cell preservation arrangement of claim 8 , wherein
the arrangement comprises a substrate for culturing biological cells, the substrate being arranged within the chamber, and
the substrate is adapted to retain the cells on the substrate, wherein the substrate forms at least part of a chip for organ-on-a-chip cell growth.

10. The biological cell preservation arrangement of claim 1 , wherein the arrangement is a blood storage arrangement and wherein the deformable membrane is for agitating blood stored within the fluid retaining space to prevent activation of platelets within the blood and/or to prevent coagulation of the blood, and wherein the blood storage arrangement comprises a blood storage bag, the bag forming the chamber.

11. The biological cell preservation arrangement of claim 10 , wherein
the deformable membrane forms at least a portion of an enclosing wall of the fluid retaining space, and
the electroactive polymer actuator arrangement comprises an actuation layer comprising an electroactive polymer, the layer being disposed in engaging fashion against the deformable membrane forming a wall of the fluid retaining space for deforming the membrane.

12. The biological cell preservation arrangement of claim 1 , wherein the electroactive polymer actuator arrangement comprises an electrostrictive electroactive polymer material.

13. The biological cell preservation arrangement of claim 5 , wherein the controller is adapted to recurringly control the actuator elements to actuate in a cascading fashion wherein the actuators are actuated in sequential consecutive groups, each actuated group being larger than the previously actuated group.

14. The biological cell preservation arrangement of claim 1 , wherein:
the electroactive polymer actuator arrangement comprises a first actuator arrangement and a second actuator arrangement,
the first actuator arrangement is arranged on a first surface of the chamber,
the second actuator arrangement is arranged on a second surface of the chamber, opposite the first surface,
the controller changes the topology of the first surface to induce the flow of the fluid in a first direction,
the controller changes the topology of the second surface to induce the flow of the fluid in a second direction,
wherein the second direction is opposite the first direction, thereby causing a circular flow of the fluid within the chamber.

15. A biological container comprising:
a fluidically closable chamber comprising a fluid retaining space configured to retain a volume of fluid comprising biological material;
wherein the chamber comprises one or more deformable membranes;
a first electroactive polymer actuator arrangement;
a second electroactive polymer actuator arrangement; and
a controller;
wherein the first and second electroactive polymer actuator arrangements are situated at opposing first and second surfaces of the chamber;
wherein the controller is configured to control the first and second electroactive polymer actuator arrangements to change a first topology of the first surface and a second topology of the second surface;
wherein the controller is configured to change the first topology and the second topology to induce a first flow of the fluid in a first direction parallel to the first surface, and a second flow of the fluid in a second direction parallel to the second surface;
wherein the first direction is opposite to the second direction, thereby inducing a circular flow of the fluid within the chamber.

16. The biological container of claim 15 , wherein each of the first and second electroactive polymer actuator arrangements comprises an array of actuator elements, wherein each actuator element comprises an electroactive polymer that is actuable to undergo a bending action.

17. The biological container of claim 16 , wherein the controller:
selectively controls the actuator elements of the first electroactive polymer actuator arrangement in a first ordered sequence that causes the fluid flow in the first direction to be in a direction corresponding to the first ordered sequence, and
selectively controls the actuator elements of the second electroactive polymer actuator arrangement in a second ordered sequence that causes the fluid flow in the second direction to be in a direction corresponding to the second ordered sequence.

18. The biological container of claim 17 , wherein the first ordered sequence and second ordered sequence provide changes to the first topology and the second topology that maintain a substantially constant volume of the chamber.

Assignees

• Koninklijke Philips Nv

Inventors

• Pelssers Eduard Gerard Marie
• Hendriks Cornelis Petrus
• Wimberger-Friedl Reinhold
• Johnson Mark Thomas
• Hilgers Achim

Classifications

• A01N1/146

  Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving · CPC title

• B01F2101/44

  Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation · CPC title

• B01F31/312

  the motion being a transversal movement to one part of the receptacle, e.g. by moving alternatively up and down the opposite edges of a closing lid to cause a pumping action · CPC title

• C12M41/42

  of agitation speed · CPC title

• C12M25/00

  Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings · CPC title