Rapid antibiotic susceptibility testing system based on bacterial immobilization using gelling agent, antibiotic diffusion and tracking of single bacterial cells
US-9133498-B2 · Sep 15, 2015 · US
Method for isolation and morphological analysis of cells
US12059682B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12059682-B2 |
| Application number | US-201816166209-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 22, 2018 |
| Priority date | Oct 22, 2018 |
| Publication date | Aug 13, 2024 |
| Grant date | Aug 13, 2024 |
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- Title
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Abstract
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Provided is a method for cell isolation and morphological analysis. The method includes: providing a substrate with one or more wells; inserting cell isolation devices, each of which has one side patterned with one or more microwells, into the wells; introducing a liquid medium including cells into the wells; allowing the microwells of the cell isolation devices to come into contact with the inner surfaces of the wells such that some of the cells are isolated and trapped at the level of individual cells in the microwells; and observing the morphological changes of the isolated cells.
First claim
Opening claim text (preview).
What is claimed is: 1. A cell isolation device for cell analysis comprising: a body ( 120 ), wherein, the body ( 120 ) comprises a top portion ( 121 ) and a bottom portion ( 122 ), wherein the top portion ( 121 ) comprises a top surface ( 123 ) and an outer edge portion ( 125 ) and the bottom portion ( 122 ) has a bottom surface ( 124 ), the body ( 120 ) is made of a swellable material, bubble holes ( 126 ) are formed on a flank of the top portion ( 121 ), one or more microwells are patterned at least on the bottom surface ( 124 ), and the bottom portion and the top portion have a cylindrical shape, and the bottom portion ( 122 ) has a smaller diameter than the top portion ( 121 ). 2. The cell isolation device according to claim 1 , wherein the swellable material is capable of being swollen by an external stimulus, wherein the external stimulus is selected from the group consisting of electricity, light, sound, heat, magnetism, specific substances, and combinations thereof. 3. The cell isolation device according to claim 1 , wherein the swellable material is a hydrogel. 4. The cell isolation device according to claim 1 , wherein the top surface ( 123 ) is concave. 5. A kit for cell isolation and morphological analysis comprising a substrate having one or more wells and a plurality of the cell isolation devices and each of the plurality of the cell isolation devices is according to claim 1 and is inserted and arranged in each well of the substrate.
Assignees
Inventors
Classifications
Multi-well plates; Microtitration plates · CPC title
Bottom walls · CPC title
Absorbents; Gels to retain a fluid · CPC title
Surface properties and coatings · CPC title
by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title
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- What does patent US12059682B2 cover?
- Provided is a method for cell isolation and morphological analysis. The method includes: providing a substrate with one or more wells; inserting cell isolation devices, each of which has one side patterned with one or more microwells, into the wells; introducing a liquid medium including cells into the wells; allowing the microwells of the cell isolation devices to come into contact with the in…
- Who is the assignee on this patent?
- Ezdiatech Inc, Univ Industry Cooperation Group Kyung Hee Univ, Univ—Industry Cooperation Group Of Kyung Hee Univ
- What technology area does this patent fall under?
- Primary CPC classification B01L3/5085. Mapped technology areas include Operations & Transport.
- When was this patent published?
- Publication date Tue Aug 13 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).