Culture method for differentiating primordial germ cells into functionally mature oocytes
US-2018251729-A1 · Sep 6, 2018 · US
Method of differentiating primordial germ cell into primordial follicle in vitro
US12054747B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-12054747-B2 |
| Application number | US-201917253518-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 29, 2019 |
| Priority date | Jun 21, 2018 |
| Publication date | Aug 6, 2024 |
| Grant date | Aug 6, 2024 |
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Abstract
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A method of differentiating a primordial germ cell into a primordial follicle in vitro includes culturing a primordial germ cell and a supporting cell adjacent to the primordial germ cell under a pressurized condition or a low oxygen concentration condition.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of differentiating a primordial germ cell into a primordial follicle in vitro, the method comprising: culturing a primordial germ cell and a supporting cell adjacent to the primordial germ cell under a pressurized condition of between 23 kPa and 40 kPa, or an atmospheric oxygen concentration of 7% or lower. 2. The method according to claim 1 , wherein the culturing is carried out under the pressurized condition and atmospheric oxygen concentrations. 3. The method according to claim 1 , wherein the culturing is carried out in the presence of a PI3 kinase inhibitor. 4. The method according to claim 3 , wherein the PI3 kinase inhibitor is LY294002. 5. The method according to claim 1 , wherein the culturing is carried out in the presence of an estrogen inhibitor. 6. The method according to claim 1 , wherein the low oxygen concentration condition is a condition in which an oxygen concentration in a culture atmosphere is between 3% and 7%. 7. The method according to claim 1 , wherein the pressurized condition is between 28 kPa and 38 kPa. 8. The method according to claim 1 , wherein the obtained primordial follicle is formed of flat granulosa cells and an oocyte surrounded by the flat granulosa cells, and a Foxo3a is localized in the nucleus of the oocyte. 9. The method according to claim 8 , wherein the obtained primordial follicle has a diameter of 20 μm or smaller.
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Culture process characterised by the use of hydrostatic pressure, flow or shear forces · CPC title
from germ cells · CPC title
Kinases (EC 2.7.) · CPC title
Regulators of development · CPC title
Atmosphere, e.g. low oxygen conditions · CPC title
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- What does patent US12054747B2 cover?
- A method of differentiating a primordial germ cell into a primordial follicle in vitro includes culturing a primordial germ cell and a supporting cell adjacent to the primordial germ cell under a pressurized condition or a low oxygen concentration condition.
- Who is the assignee on this patent?
- Univ Kyushu Nat Univ Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0682. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 06 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).