Pesticidal genes and methods of use

Patent metadata

Field	Value
Publication number	US-12054730-B2
Application number	US-202117329940-A
Country	US
Kind code	B2
Filing date	May 25, 2021
Priority date	Apr 11, 2017
Publication date	Aug 6, 2024
Grant date	Aug 6, 2024

Abstract

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest, including microorganisms and plants. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

First claim

That which is claimed is:

1. A recombinant polypeptide having pesticidal activity, said polypeptide comprising an amino acid sequence having at least 95% sequence identity to amino acid sequence set forth in SEQ ID NO: 24 or 25, wherein said polypeptide has pesticidal activity, and
wherein said polypeptide further comprises a heterologous amino acid sequence chemically linked to said polypeptide.

2. A composition comprising the polypeptide of claim 1 .

3. A recombinant nucleic acid molecule encoding a polypeptide comprising:
an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID No: 24 or 25; wherein said polypeptide has pesticidal activity;
wherein said recombinant nucleic acid molecule is operably linked to a heterologous promoter.

4. The recombinant nucleic acid molecule of claim 3 , wherein said recombinant nucleic acid molecule is a synthetic sequence designed for expression in a plant.

5. The recombinant nucleic acid molecule of claim 3 , wherein said heterologous promoter is capable of directing expression in a plant cell.

6. The recombinant nucleic acid molecule of claim 3 , wherein said heterologous promoter is capable of directing expression in a bacterium.

7. A host cell comprising the recombinant nucleic acid molecule of claim 3 .

8. The host cell of claim 7 , wherein said host cell is a bacterial host cell.

9. A DNA construct comprising a promoter that drives expression in a plant cell operably linked to a recombinant nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 24 or 25, wherein said polypeptide has pesticidal activity.

10. The DNA construct of claim 9 , wherein said nucleotide sequence is a synthetic DNA sequence that has been designed for expression in a plant.

11. A vector comprising the DNA construct of claim 10 .

12. A host cell comprising the vector of claim 11 .

13. The host cell of claim 12 , wherein the host cell is a plant cell.

14. A transgenic plant comprising the host cell of claim 11 .

15. A composition comprising the host cell of claim 8 .

16. The composition of claim 15 , wherein said composition is selected from the group consisting of a powder, dust, pellet, granule, spray, emulsion, colloid, and solution.

17. A method for producing a polypeptide with pesticidal activity, comprising culturing the host cell of claim 7 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.

18. A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a polypeptide having pesticidal activity, wherein said polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID No: 24 or 25, wherein said polypeptide has pesticidal activity.

19. A transgenic seed of the plant of claim 18 .

20. A method for protecting a plant from an insect pest, said method comprising growing a plant or cell thereof that expresses a nucleotide sequence that encodes a pesticidal polypeptide, wherein said pesticidal polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID No: 24 or 25, wherein said pesticidal polypeptide has pesticidal activity.

21. The method of claim 20 , wherein said plant or cell thereof produces a pesticidal polypeptide having pesticidal activity against a lepidopteran, a hemipteran, and/or a coleopteran pest.

22. A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleotide sequence that encodes a pesticidal polypeptide, wherein said pesticidal polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 24 or 25,
wherein said pesticidal polypeptide has pesticidal activity, and
Wherein the field is infested with a pest against which said polypeptide has pesticidal activity.

23. The recombinant polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 24 or 25.

24. The recombinant nucleic acid molecule of claim 3 , wherein the recombinant nucleic acid molecule encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 24 or 25.

25. The DNA construct of claim 9 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 24 or 25.

26. The plant of claim 18 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 24 or 25.

27. The method of claim 20 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 24 or 25.

28. The method of claim 22 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 24 or 25.

Assignees

• Agbiome Inc

Inventors

• Parks Jessica
• Roberts Kira Bulazel
• Thayer Rebecca E

Classifications

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C07K14/325

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• C07K14/32

  from Bacillus (G) · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title