Protein signatures for distinguishing between bacterial and viral infections

Patent metadata

Field	Value
Publication number	US-12044681-B2
Application number	US-202117372575-A
Country	US
Kind code	B2
Filing date	Jul 12, 2021
Priority date	Jul 10, 2016
Publication date	Jul 23, 2024
Grant date	Jul 23, 2024

Abstract

Methods of diagnosing infections are disclosed. In one embodiment, the method comprises measuring the amount of each of the polypeptides TRAIL, CRP, IP10 and at least one additional polypeptide selected from the group consisting of IL-6 and PCT.

First claim

What is claimed is:

1. A kit for distinguishing between a viral and a bacterial infection using a blood sample of a subject comprising:
(i) an antibody which specifically detects TNF-related apoptosis-inducing ligand (TRAIL);
(ii) an antibody which specifically detects Interleukin-6 (IL-6):
(iii) an antibody which specifically detects C-reactive protein (CRP); and
(iv) an antibody which specifically detects Interferon gamma-induced protein 10 (IP10), wherein the kit comprises antibodies that specifically detect no more than 5 proteins, wherein at least one of said antibodies is attached to a detectable label or a solid support.

2. The kit of claim 1 , comprising antibodies which specifically detect said TRAIL, said IP10, said CRP, said IL-6 and Procalcitonin (PCT).

3. The kit of claim 1 , wherein each of said antibodies are attached to a detectable moiety.

4. The kit of claim 1 , wherein each of said antibodies are attached to a solid support.

5. The kit of claim 1 , wherein the antibodies of the kit consist of:
(i) said antibody which specifically detects TRAIL;
(ii) said antibody which specifically detects IL-6:
(iii) said antibody which specifically detects CRP; and
(iv) said antibody which specifically detects IP10.

6. The kit of claim 1 , wherein the antibodies of the kit consist of:
(i) said antibody which specifically detects TRAIL;
(ii) said antibody which specifically detects IL-6;
(iii) an antibody which specifically detects PCT;
(iii) said antibody which specifically detects CRP; and
(iv) said antibody which specifically detects IP10.

7. The kit of claim 1 , wherein said detectable label is selected from the group consisting of a radioactive label, a fluorescent label, a chemiluminescent label, a colorimetric label and an enzyme.

8. The kit of claim 7 , wherein said enzyme is horseradish peroxidase or alkaline phosphatase.

9. The kit of claim 1 , wherein at least one of said antibodies is a monoclonal antibody.

Assignees

• Memed Diagnostics Ltd

Inventors

• Eden Eran
• Oved Kfir
• Cohen-Dotan Assaf
• Navon Roy
• Boico Olga
• Kronenfeld Gali
• Paz Meital
• Bamberger Ellen

Classifications

• G01N2469/00

  Immunoassays for the detection of microorganisms · CPC title

• G01N2333/70578

  NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30 CD40 or CD95 · CPC title

• G01N2333/5753

  Calcitonin gene related peptide · CPC title

• G01N2333/5412

  IL-6 · CPC title

• G01N33/577

  involving monoclonal antibodies {binding reaction mechanisms characterised by the use of monoclonal antibodies (G01N33/5302 - G01N33/576 take precedence)} · CPC title